Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.

Autores
Murcia, Germán; Retamales, Martín; Pontin, Mariela Ana; Fontana, Ariel Ramón; Bottini, Rubén; Piccoli, Patricia Noemí
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
ABA and gibberellins are terpenic phytohormones that modulate several physiological responses. In many species the resistance to stress conditions is related with increases of secondary metabolites, including polyphenols and terpenes (particularly ABA), and the amino acid proline. This study investigated polyphenol, terpene and amino acid profiles in grape plants submitted to ABA and GA3 application. By GC–EIMS analysis of berry, leaf and roots 19 terpenes, including monoterpenes, sesquiterpenes, diterpenes and triterpenes were characterized. Also, 14 amino acids were identified, with L-Glutamic acid and L-Aspartic acid, both related with antioxidant defense, as the most abundant in leaf tissues. By HPLC-MWD the anthocyanin polyphenol profile was assessed. Applications of the two phytohormones decreased the free amino acid levels, presumably used as precursors of secondary metabolites; especially triterpenes involved in acclimation to stress that were augmented. However, L-Glutamic acid and L-Aspartic acid were specifically enhanced after hormonal treatment. ABA applications improved synthesis of polyphenols with antioxidant properties suggesting mechanisms of defense against oxidative damage. Also, L-Proline accumulated whereas L-Phenylalanine was decreased, in concordance with literature showing an osmotic adjustment in water deficit-stressed plants.
Fil: Murcia, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Retamales, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
XV Congreso Latinoamericano; XXX Reunión Argentina de Fisiología Vegetal
Mar del Plata
Argentina
Asociación Sociedad Argentina de Fisiología Vegetal
Materia
no contiene
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197912

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network_name_str CONICET Digital (CONICET)
spelling Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.Murcia, GermánRetamales, MartínPontin, Mariela AnaFontana, Ariel RamónBottini, RubénPiccoli, Patricia Noemíno contienehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1ABA and gibberellins are terpenic phytohormones that modulate several physiological responses. In many species the resistance to stress conditions is related with increases of secondary metabolites, including polyphenols and terpenes (particularly ABA), and the amino acid proline. This study investigated polyphenol, terpene and amino acid profiles in grape plants submitted to ABA and GA3 application. By GC–EIMS analysis of berry, leaf and roots 19 terpenes, including monoterpenes, sesquiterpenes, diterpenes and triterpenes were characterized. Also, 14 amino acids were identified, with L-Glutamic acid and L-Aspartic acid, both related with antioxidant defense, as the most abundant in leaf tissues. By HPLC-MWD the anthocyanin polyphenol profile was assessed. Applications of the two phytohormones decreased the free amino acid levels, presumably used as precursors of secondary metabolites; especially triterpenes involved in acclimation to stress that were augmented. However, L-Glutamic acid and L-Aspartic acid were specifically enhanced after hormonal treatment. ABA applications improved synthesis of polyphenols with antioxidant properties suggesting mechanisms of defense against oxidative damage. Also, L-Proline accumulated whereas L-Phenylalanine was decreased, in concordance with literature showing an osmotic adjustment in water deficit-stressed plants.Fil: Murcia, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Retamales, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Bottini, Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaXV Congreso Latinoamericano; XXX Reunión Argentina de Fisiología VegetalMar del PlataArgentinaAsociación Sociedad Argentina de Fisiología VegetalUniversidad Nacional de Mar del PlataPereyra Irujo, Gustavo AdrianPaz, Cosme Daniel2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197912Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.; XV Congreso Latinoamericano; XXX Reunión Argentina de Fisiología Vegetal; Mar del Plata; Argentina; 2014; 63-63978-987-544-592-5CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://fisiologiavegetal2014.files.wordpress.com/2018/08/libro.pdfhttps://www.youtube.com/watch?v=mp6DxuPEurEInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:16Zoai:ri.conicet.gov.ar:11336/197912instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:16.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
title Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
spellingShingle Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
Murcia, Germán
no contiene
title_short Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
title_full Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
title_fullStr Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
title_full_unstemmed Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
title_sort Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.
dc.creator.none.fl_str_mv Murcia, Germán
Retamales, Martín
Pontin, Mariela Ana
Fontana, Ariel Ramón
Bottini, Rubén
Piccoli, Patricia Noemí
author Murcia, Germán
author_facet Murcia, Germán
Retamales, Martín
Pontin, Mariela Ana
Fontana, Ariel Ramón
Bottini, Rubén
Piccoli, Patricia Noemí
author_role author
author2 Retamales, Martín
Pontin, Mariela Ana
Fontana, Ariel Ramón
Bottini, Rubén
Piccoli, Patricia Noemí
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Pereyra Irujo, Gustavo Adrian
Paz, Cosme Daniel
dc.subject.none.fl_str_mv no contiene
topic no contiene
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv ABA and gibberellins are terpenic phytohormones that modulate several physiological responses. In many species the resistance to stress conditions is related with increases of secondary metabolites, including polyphenols and terpenes (particularly ABA), and the amino acid proline. This study investigated polyphenol, terpene and amino acid profiles in grape plants submitted to ABA and GA3 application. By GC–EIMS analysis of berry, leaf and roots 19 terpenes, including monoterpenes, sesquiterpenes, diterpenes and triterpenes were characterized. Also, 14 amino acids were identified, with L-Glutamic acid and L-Aspartic acid, both related with antioxidant defense, as the most abundant in leaf tissues. By HPLC-MWD the anthocyanin polyphenol profile was assessed. Applications of the two phytohormones decreased the free amino acid levels, presumably used as precursors of secondary metabolites; especially triterpenes involved in acclimation to stress that were augmented. However, L-Glutamic acid and L-Aspartic acid were specifically enhanced after hormonal treatment. ABA applications improved synthesis of polyphenols with antioxidant properties suggesting mechanisms of defense against oxidative damage. Also, L-Proline accumulated whereas L-Phenylalanine was decreased, in concordance with literature showing an osmotic adjustment in water deficit-stressed plants.
Fil: Murcia, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Retamales, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
XV Congreso Latinoamericano; XXX Reunión Argentina de Fisiología Vegetal
Mar del Plata
Argentina
Asociación Sociedad Argentina de Fisiología Vegetal
description ABA and gibberellins are terpenic phytohormones that modulate several physiological responses. In many species the resistance to stress conditions is related with increases of secondary metabolites, including polyphenols and terpenes (particularly ABA), and the amino acid proline. This study investigated polyphenol, terpene and amino acid profiles in grape plants submitted to ABA and GA3 application. By GC–EIMS analysis of berry, leaf and roots 19 terpenes, including monoterpenes, sesquiterpenes, diterpenes and triterpenes were characterized. Also, 14 amino acids were identified, with L-Glutamic acid and L-Aspartic acid, both related with antioxidant defense, as the most abundant in leaf tissues. By HPLC-MWD the anthocyanin polyphenol profile was assessed. Applications of the two phytohormones decreased the free amino acid levels, presumably used as precursors of secondary metabolites; especially triterpenes involved in acclimation to stress that were augmented. However, L-Glutamic acid and L-Aspartic acid were specifically enhanced after hormonal treatment. ABA applications improved synthesis of polyphenols with antioxidant properties suggesting mechanisms of defense against oxidative damage. Also, L-Proline accumulated whereas L-Phenylalanine was decreased, in concordance with literature showing an osmotic adjustment in water deficit-stressed plants.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197912
Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.; XV Congreso Latinoamericano; XXX Reunión Argentina de Fisiología Vegetal; Mar del Plata; Argentina; 2014; 63-63
978-987-544-592-5
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197912
identifier_str_mv Application of ABA and GA3 modulate synthesis of polyphenols, terpenes and free amino acids in Vitis Vinifera L.; XV Congreso Latinoamericano; XXX Reunión Argentina de Fisiología Vegetal; Mar del Plata; Argentina; 2014; 63-63
978-987-544-592-5
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://fisiologiavegetal2014.files.wordpress.com/2018/08/libro.pdf
https://www.youtube.com/watch?v=mp6DxuPEurE
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad Nacional de Mar del Plata
publisher.none.fl_str_mv Universidad Nacional de Mar del Plata
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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