Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine

Autores
Mignaqui, Ana Clara; Ferella, Alejandra; Cass, Brian; Mukankurayija, Larissa; L'Abbé, Denis; Bisson, Louis; Sánchez, Cintia Mariana; Scian, Romina; Cardillo, Sabrina Beatriz; Durocher, Yves; Wigdorovitz, Andrés
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Inactivated Foot-and-Mouth Disease (FMD) vaccine has proven to be effective in the control of the disease. However, its production has some disadvantages, including the costly biosafety facilities required for the production of huge amounts of growing live virus, the need of an exhaustive purification process to eliminate non-structural proteins of the virus in the final formulations in order to differentiate infected from vaccinated animals and variable local regulatory restrictions to produce and commercialize the vaccine. Thus, a novel vaccine against FMD that overcome these restrictions is desirable. Although many developments have been made in this regard, most of them failed in terms of efficacy or when considering their transferability to the industry. We have previously reported the use of transient gene expression in mammalian cells to produce FMD virus-like particles (VLPs) as a novel vaccine for FMD and demonstrated the immunogenicity of the recombinant structures in animal models. Here, we report the optimization of the production system by assaying different DNA:polyethylenimine concentrations, cell densities, and direct and indirect protocols of transfection. Also, we evaluated the reproducibility and scalability of the technology to produce high yields of recombinant VLPs in a cost-effective and scalable system compatible with industrial tech-transfer of an effective and safe vaccine.
Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ferella, Alejandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Cass, Brian. Human Health Therapeutics Research Center; Canadá
Fil: Mukankurayija, Larissa. Human Health Therapeutics Research Center; Canadá
Fil: L'Abbé, Denis. Human Health Therapeutics Research Center; Canadá
Fil: Bisson, Louis. Human Health Therapeutics Research Center; Canadá
Fil: Sánchez, Cintia Mariana. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scian, Romina. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardillo, Sabrina Beatriz. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durocher, Yves. Human Health Therapeutics Research Center; Canadá
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Materia
EMERGENCY VACCINE
FMDV
MAMMALIAN CELLS
TRANSIENT GENE EXPRESSION
VLPS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170623
Acceder
id CONICETDig_24e75f5fb8b815bb52a15422fe3a06c2
oai_identifier_str oai:ri.conicet.gov.ar:11336/170623
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation VaccineMignaqui, Ana ClaraFerella, AlejandraCass, BrianMukankurayija, LarissaL'Abbé, DenisBisson, LouisSánchez, Cintia MarianaScian, RominaCardillo, Sabrina BeatrizDurocher, YvesWigdorovitz, AndrésEMERGENCY VACCINEFMDVMAMMALIAN CELLSTRANSIENT GENE EXPRESSIONVLPShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Inactivated Foot-and-Mouth Disease (FMD) vaccine has proven to be effective in the control of the disease. However, its production has some disadvantages, including the costly biosafety facilities required for the production of huge amounts of growing live virus, the need of an exhaustive purification process to eliminate non-structural proteins of the virus in the final formulations in order to differentiate infected from vaccinated animals and variable local regulatory restrictions to produce and commercialize the vaccine. Thus, a novel vaccine against FMD that overcome these restrictions is desirable. Although many developments have been made in this regard, most of them failed in terms of efficacy or when considering their transferability to the industry. We have previously reported the use of transient gene expression in mammalian cells to produce FMD virus-like particles (VLPs) as a novel vaccine for FMD and demonstrated the immunogenicity of the recombinant structures in animal models. Here, we report the optimization of the production system by assaying different DNA:polyethylenimine concentrations, cell densities, and direct and indirect protocols of transfection. Also, we evaluated the reproducibility and scalability of the technology to produce high yields of recombinant VLPs in a cost-effective and scalable system compatible with industrial tech-transfer of an effective and safe vaccine.Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Ferella, Alejandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; ArgentinaFil: Cass, Brian. Human Health Therapeutics Research Center; CanadáFil: Mukankurayija, Larissa. Human Health Therapeutics Research Center; CanadáFil: L'Abbé, Denis. Human Health Therapeutics Research Center; CanadáFil: Bisson, Louis. Human Health Therapeutics Research Center; CanadáFil: Sánchez, Cintia Mariana. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scian, Romina. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardillo, Sabrina Beatriz. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Durocher, Yves. Human Health Therapeutics Research Center; CanadáFil: Wigdorovitz, Andrés. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; ArgentinaFrontiers Media2020-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170623Mignaqui, Ana Clara; Ferella, Alejandra; Cass, Brian; Mukankurayija, Larissa; L'Abbé, Denis; et al.; Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine; Frontiers Media; Frontiers in Veterinary Science; 7; 601; 23-9-2020; 1-92297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fvets.2020.00601/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.00601info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:52Zoai:ri.conicet.gov.ar:11336/170623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:52.728CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
title Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
spellingShingle Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
Mignaqui, Ana Clara
EMERGENCY VACCINE
FMDV
MAMMALIAN CELLS
TRANSIENT GENE EXPRESSION
VLPS
title_short Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
title_full Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
title_fullStr Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
title_full_unstemmed Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
title_sort Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
dc.creator.none.fl_str_mv Mignaqui, Ana Clara
Ferella, Alejandra
Cass, Brian
Mukankurayija, Larissa
L'Abbé, Denis
Bisson, Louis
Sánchez, Cintia Mariana
Scian, Romina
Cardillo, Sabrina Beatriz
Durocher, Yves
Wigdorovitz, Andrés
author Mignaqui, Ana Clara
author_facet Mignaqui, Ana Clara
Ferella, Alejandra
Cass, Brian
Mukankurayija, Larissa
L'Abbé, Denis
Bisson, Louis
Sánchez, Cintia Mariana
Scian, Romina
Cardillo, Sabrina Beatriz
Durocher, Yves
Wigdorovitz, Andrés
author_role author
author2 Ferella, Alejandra
Cass, Brian
Mukankurayija, Larissa
L'Abbé, Denis
Bisson, Louis
Sánchez, Cintia Mariana
Scian, Romina
Cardillo, Sabrina Beatriz
Durocher, Yves
Wigdorovitz, Andrés
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EMERGENCY VACCINE
FMDV
MAMMALIAN CELLS
TRANSIENT GENE EXPRESSION
VLPS
topic EMERGENCY VACCINE
FMDV
MAMMALIAN CELLS
TRANSIENT GENE EXPRESSION
VLPS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Inactivated Foot-and-Mouth Disease (FMD) vaccine has proven to be effective in the control of the disease. However, its production has some disadvantages, including the costly biosafety facilities required for the production of huge amounts of growing live virus, the need of an exhaustive purification process to eliminate non-structural proteins of the virus in the final formulations in order to differentiate infected from vaccinated animals and variable local regulatory restrictions to produce and commercialize the vaccine. Thus, a novel vaccine against FMD that overcome these restrictions is desirable. Although many developments have been made in this regard, most of them failed in terms of efficacy or when considering their transferability to the industry. We have previously reported the use of transient gene expression in mammalian cells to produce FMD virus-like particles (VLPs) as a novel vaccine for FMD and demonstrated the immunogenicity of the recombinant structures in animal models. Here, we report the optimization of the production system by assaying different DNA:polyethylenimine concentrations, cell densities, and direct and indirect protocols of transfection. Also, we evaluated the reproducibility and scalability of the technology to produce high yields of recombinant VLPs in a cost-effective and scalable system compatible with industrial tech-transfer of an effective and safe vaccine.
Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ferella, Alejandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Cass, Brian. Human Health Therapeutics Research Center; Canadá
Fil: Mukankurayija, Larissa. Human Health Therapeutics Research Center; Canadá
Fil: L'Abbé, Denis. Human Health Therapeutics Research Center; Canadá
Fil: Bisson, Louis. Human Health Therapeutics Research Center; Canadá
Fil: Sánchez, Cintia Mariana. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scian, Romina. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardillo, Sabrina Beatriz. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durocher, Yves. Human Health Therapeutics Research Center; Canadá
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
description Inactivated Foot-and-Mouth Disease (FMD) vaccine has proven to be effective in the control of the disease. However, its production has some disadvantages, including the costly biosafety facilities required for the production of huge amounts of growing live virus, the need of an exhaustive purification process to eliminate non-structural proteins of the virus in the final formulations in order to differentiate infected from vaccinated animals and variable local regulatory restrictions to produce and commercialize the vaccine. Thus, a novel vaccine against FMD that overcome these restrictions is desirable. Although many developments have been made in this regard, most of them failed in terms of efficacy or when considering their transferability to the industry. We have previously reported the use of transient gene expression in mammalian cells to produce FMD virus-like particles (VLPs) as a novel vaccine for FMD and demonstrated the immunogenicity of the recombinant structures in animal models. Here, we report the optimization of the production system by assaying different DNA:polyethylenimine concentrations, cell densities, and direct and indirect protocols of transfection. Also, we evaluated the reproducibility and scalability of the technology to produce high yields of recombinant VLPs in a cost-effective and scalable system compatible with industrial tech-transfer of an effective and safe vaccine.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170623
Mignaqui, Ana Clara; Ferella, Alejandra; Cass, Brian; Mukankurayija, Larissa; L'Abbé, Denis; et al.; Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine; Frontiers Media; Frontiers in Veterinary Science; 7; 601; 23-9-2020; 1-9
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170623
identifier_str_mv Mignaqui, Ana Clara; Ferella, Alejandra; Cass, Brian; Mukankurayija, Larissa; L'Abbé, Denis; et al.; Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine; Frontiers Media; Frontiers in Veterinary Science; 7; 601; 23-9-2020; 1-9
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fvets.2020.00601/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.00601
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613878212722688
score 13.070432

Publicaciones similares