A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants

Autores
Dus Santos, María José; Wigdorovitz, Andrés; Trono, Karina Gabriela; Ríos, Raúl D.; Franzone, Pascual M.; Gil, Felix; Moreno, Javier Edgardo; Carrillo, Consuelo; Escribano, José M.; Borca, Manuel V.
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The expression of antigens in transgenic plants has been increasingly used as an alternative to the classical methodologies for antigen expression in the development of experimental vaccines. However, an important limitation in most cases is the low concentration of the recombinant antigens in the plant tissues, which reduces the possibilities of practical applications. Because the site of insertion of the transferred DNA into the cellular chromosomal DNA is at random, different levels of foreign protein expression in independent transformants is expected. Strategies to allow the evaluation of a high number of the transgenic individuals, usually an expensive and very time consuming process, would permit the selection of those plants presenting the highest levels of recombinant protein expression. Here, we present the development of an experimental immunogen based in the expression of a highly immunogenic epitope from foot and mouth disease virus (FMDV) fused to the glucuronidase (gus A) reporter gene, which allows selection of the transgenic plants by the ß-glucuronidase (ßGUS) enzymatic activity. We produced transgenic plants of alfalfa expressing the immunogenic site between amino acid residues 135–160 of structural protein VP1 (VP135–160), fused to the ßGUS protein. Plants expressing the highest levels of the immunogenic epitope VP135–160, analyzed by Western blot, were efficiently selected based on their levels of ßGUS enzymatic activity. The FMDV epitope expressed in plants was highly immunogenic in mice which developed, after immunization, a strong anti-FMDV antibody response against a synthetic peptide representing the region VP135–160, to native virus VP1, and to purified FMDV particles. Additionally, these mice were completely protected against experimental challenge with the virulent virus. To our knowledge, this constitutes the first report of a peptide-based vaccine produced in transgenic plants that induces a protective immune response when used in experimental hosts. Also, these results demonstrated the possibility of using a novel and simple methodology for obtaining transgenic plants expressing high levels of foreign immunogenic epitopes, which could be directly applied in the development of plant-based vaccines.
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ríos, Raúl D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Franzone, Pascual M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Gil, Felix. Centro de Investigación en Sanidad Animal; España
Fil: Moreno, Javier Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrillo, Consuelo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Escribano, José M.. Centro de Investigación en Sanidad Animal; España
Fil: Borca, Manuel V.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Materia
ANTIGEN EXPRESSION
FMDV
RECOMBINANT PLANTS
VACCINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94344
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94344
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plantsDus Santos, María JoséWigdorovitz, AndrésTrono, Karina GabrielaRíos, Raúl D.Franzone, Pascual M.Gil, FelixMoreno, Javier EdgardoCarrillo, ConsueloEscribano, José M.Borca, Manuel V.ANTIGEN EXPRESSIONFMDVRECOMBINANT PLANTSVACCINATIONhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4The expression of antigens in transgenic plants has been increasingly used as an alternative to the classical methodologies for antigen expression in the development of experimental vaccines. However, an important limitation in most cases is the low concentration of the recombinant antigens in the plant tissues, which reduces the possibilities of practical applications. Because the site of insertion of the transferred DNA into the cellular chromosomal DNA is at random, different levels of foreign protein expression in independent transformants is expected. Strategies to allow the evaluation of a high number of the transgenic individuals, usually an expensive and very time consuming process, would permit the selection of those plants presenting the highest levels of recombinant protein expression. Here, we present the development of an experimental immunogen based in the expression of a highly immunogenic epitope from foot and mouth disease virus (FMDV) fused to the glucuronidase (gus A) reporter gene, which allows selection of the transgenic plants by the ß-glucuronidase (ßGUS) enzymatic activity. We produced transgenic plants of alfalfa expressing the immunogenic site between amino acid residues 135–160 of structural protein VP1 (VP135–160), fused to the ßGUS protein. Plants expressing the highest levels of the immunogenic epitope VP135–160, analyzed by Western blot, were efficiently selected based on their levels of ßGUS enzymatic activity. The FMDV epitope expressed in plants was highly immunogenic in mice which developed, after immunization, a strong anti-FMDV antibody response against a synthetic peptide representing the region VP135–160, to native virus VP1, and to purified FMDV particles. Additionally, these mice were completely protected against experimental challenge with the virulent virus. To our knowledge, this constitutes the first report of a peptide-based vaccine produced in transgenic plants that induces a protective immune response when used in experimental hosts. Also, these results demonstrated the possibility of using a novel and simple methodology for obtaining transgenic plants expressing high levels of foreign immunogenic epitopes, which could be directly applied in the development of plant-based vaccines.Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ríos, Raúl D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Franzone, Pascual M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Gil, Felix. Centro de Investigación en Sanidad Animal; EspañaFil: Moreno, Javier Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carrillo, Consuelo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Escribano, José M.. Centro de Investigación en Sanidad Animal; EspañaFil: Borca, Manuel V.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaElsevier2002-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94344Dus Santos, María José; Wigdorovitz, Andrés; Trono, Karina Gabriela; Ríos, Raúl D.; Franzone, Pascual M.; et al.; A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants; Elsevier; Vaccine; 20; 7-8; 1-2002; 1141-11470264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X01004340info:eu-repo/semantics/altIdentifier/doi/10.1016/S0264-410X(01)00434-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:46Zoai:ri.conicet.gov.ar:11336/94344instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:46.799CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
title A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
spellingShingle A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
Dus Santos, María José
ANTIGEN EXPRESSION
FMDV
RECOMBINANT PLANTS
VACCINATION
title_short A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
title_full A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
title_fullStr A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
title_full_unstemmed A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
title_sort A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
dc.creator.none.fl_str_mv Dus Santos, María José
Wigdorovitz, Andrés
Trono, Karina Gabriela
Ríos, Raúl D.
Franzone, Pascual M.
Gil, Felix
Moreno, Javier Edgardo
Carrillo, Consuelo
Escribano, José M.
Borca, Manuel V.
author Dus Santos, María José
author_facet Dus Santos, María José
Wigdorovitz, Andrés
Trono, Karina Gabriela
Ríos, Raúl D.
Franzone, Pascual M.
Gil, Felix
Moreno, Javier Edgardo
Carrillo, Consuelo
Escribano, José M.
Borca, Manuel V.
author_role author
author2 Wigdorovitz, Andrés
Trono, Karina Gabriela
Ríos, Raúl D.
Franzone, Pascual M.
Gil, Felix
Moreno, Javier Edgardo
Carrillo, Consuelo
Escribano, José M.
Borca, Manuel V.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIGEN EXPRESSION
FMDV
RECOMBINANT PLANTS
VACCINATION
topic ANTIGEN EXPRESSION
FMDV
RECOMBINANT PLANTS
VACCINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The expression of antigens in transgenic plants has been increasingly used as an alternative to the classical methodologies for antigen expression in the development of experimental vaccines. However, an important limitation in most cases is the low concentration of the recombinant antigens in the plant tissues, which reduces the possibilities of practical applications. Because the site of insertion of the transferred DNA into the cellular chromosomal DNA is at random, different levels of foreign protein expression in independent transformants is expected. Strategies to allow the evaluation of a high number of the transgenic individuals, usually an expensive and very time consuming process, would permit the selection of those plants presenting the highest levels of recombinant protein expression. Here, we present the development of an experimental immunogen based in the expression of a highly immunogenic epitope from foot and mouth disease virus (FMDV) fused to the glucuronidase (gus A) reporter gene, which allows selection of the transgenic plants by the ß-glucuronidase (ßGUS) enzymatic activity. We produced transgenic plants of alfalfa expressing the immunogenic site between amino acid residues 135–160 of structural protein VP1 (VP135–160), fused to the ßGUS protein. Plants expressing the highest levels of the immunogenic epitope VP135–160, analyzed by Western blot, were efficiently selected based on their levels of ßGUS enzymatic activity. The FMDV epitope expressed in plants was highly immunogenic in mice which developed, after immunization, a strong anti-FMDV antibody response against a synthetic peptide representing the region VP135–160, to native virus VP1, and to purified FMDV particles. Additionally, these mice were completely protected against experimental challenge with the virulent virus. To our knowledge, this constitutes the first report of a peptide-based vaccine produced in transgenic plants that induces a protective immune response when used in experimental hosts. Also, these results demonstrated the possibility of using a novel and simple methodology for obtaining transgenic plants expressing high levels of foreign immunogenic epitopes, which could be directly applied in the development of plant-based vaccines.
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ríos, Raúl D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Franzone, Pascual M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Gil, Felix. Centro de Investigación en Sanidad Animal; España
Fil: Moreno, Javier Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrillo, Consuelo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Escribano, José M.. Centro de Investigación en Sanidad Animal; España
Fil: Borca, Manuel V.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
description The expression of antigens in transgenic plants has been increasingly used as an alternative to the classical methodologies for antigen expression in the development of experimental vaccines. However, an important limitation in most cases is the low concentration of the recombinant antigens in the plant tissues, which reduces the possibilities of practical applications. Because the site of insertion of the transferred DNA into the cellular chromosomal DNA is at random, different levels of foreign protein expression in independent transformants is expected. Strategies to allow the evaluation of a high number of the transgenic individuals, usually an expensive and very time consuming process, would permit the selection of those plants presenting the highest levels of recombinant protein expression. Here, we present the development of an experimental immunogen based in the expression of a highly immunogenic epitope from foot and mouth disease virus (FMDV) fused to the glucuronidase (gus A) reporter gene, which allows selection of the transgenic plants by the ß-glucuronidase (ßGUS) enzymatic activity. We produced transgenic plants of alfalfa expressing the immunogenic site between amino acid residues 135–160 of structural protein VP1 (VP135–160), fused to the ßGUS protein. Plants expressing the highest levels of the immunogenic epitope VP135–160, analyzed by Western blot, were efficiently selected based on their levels of ßGUS enzymatic activity. The FMDV epitope expressed in plants was highly immunogenic in mice which developed, after immunization, a strong anti-FMDV antibody response against a synthetic peptide representing the region VP135–160, to native virus VP1, and to purified FMDV particles. Additionally, these mice were completely protected against experimental challenge with the virulent virus. To our knowledge, this constitutes the first report of a peptide-based vaccine produced in transgenic plants that induces a protective immune response when used in experimental hosts. Also, these results demonstrated the possibility of using a novel and simple methodology for obtaining transgenic plants expressing high levels of foreign immunogenic epitopes, which could be directly applied in the development of plant-based vaccines.
publishDate 2002
dc.date.none.fl_str_mv 2002-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94344
Dus Santos, María José; Wigdorovitz, Andrés; Trono, Karina Gabriela; Ríos, Raúl D.; Franzone, Pascual M.; et al.; A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants; Elsevier; Vaccine; 20; 7-8; 1-2002; 1141-1147
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94344
identifier_str_mv Dus Santos, María José; Wigdorovitz, Andrés; Trono, Karina Gabriela; Ríos, Raúl D.; Franzone, Pascual M.; et al.; A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants; Elsevier; Vaccine; 20; 7-8; 1-2002; 1141-1147
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X01004340
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0264-410X(01)00434-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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