Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice

Autores
Batista, A.; Quattrocchi, Valeria; Olivera, V.; Langellotti, Cecilia Ana; Pappalardo, Juan Sebastian; Di Giacomo, S.; Mongini, Claudia; Portuondo, D.; Zamorano, Patricia Ines
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot and Mouth Disease (FMD) is an acute disease caused by Foot and Mouth DiseaseVirus (FMDV) which causes important economy losses, this is why it is necessaryto obtain a vaccine that stimulates a rapid and long-lasting protective immuneresponse. Cliptox is a mineral microparticle that in earlier studies has shownadjuvant activity against different antigens. In this study we have examined the effects of Cliptox on the magnitude and type of immunity elicited in response to inactivated FMDV (iFMDV) vaccine. It was demonstrated that iFMDV-Cliptoxstimulates a specific antibody response detected in mucosal and in sera. Thedifferent isotype profiles elicited by inoculation with this vaccine indicate aTh1/Th2 response. Also, an increase in dendritic cells and macrophages in thespleen in comparison with the iFMDV vaccine iFMDV-Cliptox was detected. TheCliptox-iFMDV formulation was non toxic by using egg embryos and yieldedincreased protection against challenge with FMDV in the murine model. Our resultsshow that the incorporation of Cliptox into FMDVi vaccine induces an increase of the specific protective immune response in mice and clearly indicate that CliptoxTM exert an (important) up-regulation on DC and MPhi. Additionally, Cliptox TMadjuvant can be used in vaccines for induction of mucosal immune response.
Fil: Batista, A.. Toxicology and Biomedicine Center; Cuba
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivera, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pappalardo, Juan Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Di Giacomo, S.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Portuondo, D.. Toxicology and Biomedicine Center; Cuba
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina
Materia
CLIPTOX
FMDV
VACCINE
MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243751
Acceder
id CONICETDig_7ba05671c0335791a6602c4aeb741914
oai_identifier_str oai:ri.conicet.gov.ar:11336/243751
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in miceBatista, A.Quattrocchi, ValeriaOlivera, V.Langellotti, Cecilia AnaPappalardo, Juan SebastianDi Giacomo, S.Mongini, ClaudiaPortuondo, D.Zamorano, Patricia InesCLIPTOXFMDVVACCINEMICEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Foot and Mouth Disease (FMD) is an acute disease caused by Foot and Mouth DiseaseVirus (FMDV) which causes important economy losses, this is why it is necessaryto obtain a vaccine that stimulates a rapid and long-lasting protective immuneresponse. Cliptox is a mineral microparticle that in earlier studies has shownadjuvant activity against different antigens. In this study we have examined the effects of Cliptox on the magnitude and type of immunity elicited in response to inactivated FMDV (iFMDV) vaccine. It was demonstrated that iFMDV-Cliptoxstimulates a specific antibody response detected in mucosal and in sera. Thedifferent isotype profiles elicited by inoculation with this vaccine indicate aTh1/Th2 response. Also, an increase in dendritic cells and macrophages in thespleen in comparison with the iFMDV vaccine iFMDV-Cliptox was detected. TheCliptox-iFMDV formulation was non toxic by using egg embryos and yieldedincreased protection against challenge with FMDV in the murine model. Our resultsshow that the incorporation of Cliptox into FMDVi vaccine induces an increase of the specific protective immune response in mice and clearly indicate that CliptoxTM exert an (important) up-regulation on DC and MPhi. Additionally, Cliptox TMadjuvant can be used in vaccines for induction of mucosal immune response.Fil: Batista, A.. Toxicology and Biomedicine Center; CubaFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olivera, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pappalardo, Juan Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Di Giacomo, S.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Portuondo, D.. Toxicology and Biomedicine Center; CubaFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; ArgentinaElsevier2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243751Batista, A.; Quattrocchi, Valeria; Olivera, V.; Langellotti, Cecilia Ana; Pappalardo, Juan Sebastian; et al.; Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice; Elsevier; Vaccine; 28; 38; 8-2010; 6361-63660264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X10009394info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2010.06.098info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:32:27Zoai:ri.conicet.gov.ar:11336/243751instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:32:27.868CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
title Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
spellingShingle Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
Batista, A.
CLIPTOX
FMDV
VACCINE
MICE
title_short Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
title_full Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
title_fullStr Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
title_full_unstemmed Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
title_sort Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice
dc.creator.none.fl_str_mv Batista, A.
Quattrocchi, Valeria
Olivera, V.
Langellotti, Cecilia Ana
Pappalardo, Juan Sebastian
Di Giacomo, S.
Mongini, Claudia
Portuondo, D.
Zamorano, Patricia Ines
author Batista, A.
author_facet Batista, A.
Quattrocchi, Valeria
Olivera, V.
Langellotti, Cecilia Ana
Pappalardo, Juan Sebastian
Di Giacomo, S.
Mongini, Claudia
Portuondo, D.
Zamorano, Patricia Ines
author_role author
author2 Quattrocchi, Valeria
Olivera, V.
Langellotti, Cecilia Ana
Pappalardo, Juan Sebastian
Di Giacomo, S.
Mongini, Claudia
Portuondo, D.
Zamorano, Patricia Ines
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CLIPTOX
FMDV
VACCINE
MICE
topic CLIPTOX
FMDV
VACCINE
MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Foot and Mouth Disease (FMD) is an acute disease caused by Foot and Mouth DiseaseVirus (FMDV) which causes important economy losses, this is why it is necessaryto obtain a vaccine that stimulates a rapid and long-lasting protective immuneresponse. Cliptox is a mineral microparticle that in earlier studies has shownadjuvant activity against different antigens. In this study we have examined the effects of Cliptox on the magnitude and type of immunity elicited in response to inactivated FMDV (iFMDV) vaccine. It was demonstrated that iFMDV-Cliptoxstimulates a specific antibody response detected in mucosal and in sera. Thedifferent isotype profiles elicited by inoculation with this vaccine indicate aTh1/Th2 response. Also, an increase in dendritic cells and macrophages in thespleen in comparison with the iFMDV vaccine iFMDV-Cliptox was detected. TheCliptox-iFMDV formulation was non toxic by using egg embryos and yieldedincreased protection against challenge with FMDV in the murine model. Our resultsshow that the incorporation of Cliptox into FMDVi vaccine induces an increase of the specific protective immune response in mice and clearly indicate that CliptoxTM exert an (important) up-regulation on DC and MPhi. Additionally, Cliptox TMadjuvant can be used in vaccines for induction of mucosal immune response.
Fil: Batista, A.. Toxicology and Biomedicine Center; Cuba
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivera, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pappalardo, Juan Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Di Giacomo, S.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Portuondo, D.. Toxicology and Biomedicine Center; Cuba
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina
description Foot and Mouth Disease (FMD) is an acute disease caused by Foot and Mouth DiseaseVirus (FMDV) which causes important economy losses, this is why it is necessaryto obtain a vaccine that stimulates a rapid and long-lasting protective immuneresponse. Cliptox is a mineral microparticle that in earlier studies has shownadjuvant activity against different antigens. In this study we have examined the effects of Cliptox on the magnitude and type of immunity elicited in response to inactivated FMDV (iFMDV) vaccine. It was demonstrated that iFMDV-Cliptoxstimulates a specific antibody response detected in mucosal and in sera. Thedifferent isotype profiles elicited by inoculation with this vaccine indicate aTh1/Th2 response. Also, an increase in dendritic cells and macrophages in thespleen in comparison with the iFMDV vaccine iFMDV-Cliptox was detected. TheCliptox-iFMDV formulation was non toxic by using egg embryos and yieldedincreased protection against challenge with FMDV in the murine model. Our resultsshow that the incorporation of Cliptox into FMDVi vaccine induces an increase of the specific protective immune response in mice and clearly indicate that CliptoxTM exert an (important) up-regulation on DC and MPhi. Additionally, Cliptox TMadjuvant can be used in vaccines for induction of mucosal immune response.
publishDate 2010
dc.date.none.fl_str_mv 2010-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243751
Batista, A.; Quattrocchi, Valeria; Olivera, V.; Langellotti, Cecilia Ana; Pappalardo, Juan Sebastian; et al.; Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice; Elsevier; Vaccine; 28; 38; 8-2010; 6361-6366
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243751
identifier_str_mv Batista, A.; Quattrocchi, Valeria; Olivera, V.; Langellotti, Cecilia Ana; Pappalardo, Juan Sebastian; et al.; Adjuvant effect of Cliptox™ on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice; Elsevier; Vaccine; 28; 38; 8-2010; 6361-6366
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X10009394
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2010.06.098
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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