Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity

Autores
Sesto Cabral, María Eugenia; Ramos Vernieri, Alberto Nicolás; Macedo, Alexandre Jose; Trentin, Danielle; Treter, Yaninne; Manzo, Ruben Hilario; Valdez, Juan Carlos
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chronic wounds are those that remain in a chronic inflammatory state and fail to follow normal healing process. Infection is one of the most important causes of chronicity. A frequent pathogen isolated from chronic infections is Pseudomonas aeruginosa; refractory to therapy and host immune attack in its biofilm phenotype. Lactobacillus plantarum cultures supernatants (LAPS) interfere with its pathogenic capacity. In addition, LAPS showed bacteriostatic and bactericide properties and is neither cytotoxic nor an inductor of necrosis-apoptosis. LAPSs chemical composition was determined; allowing us to propose a correlation between its constituents and their biological activity. This article shows a pharmaceutical dosage form designed by using LAPS as an API with pro-healing activity and its quality control. Pharmacotechnical and anti-microbial assays were adapted to demonstrate that the vehicle used does not modify LAPS activities. Selected formulation (F100) showed fair mechanical and technological properties. From the in vitro release assays was found an adequate release from the carrier matrix and maintains its anti-pathogenic activity for 6 months. We propose F100 for chronic wounds treatment. The use of harmless bacteria by-products, such as LAPS, to antagonize infectious pathogens that have ability to form biofilm is an efficient and economic approach to treat infected chronic wounds.
Fil: Sesto Cabral, María Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ramos Vernieri, Alberto Nicolás. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Macedo, Alexandre Jose . Universidade Federal Do Rio Grande Do Sul; Brasil
Fil: Trentin, Danielle. Universidade Federal Do Rio Grande Do Sul; Brasil
Fil: Treter, Yaninne. Universidade Federal Do Rio Grande Do Sul; Brasil
Fil: Manzo, Ruben Hilario. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valdez, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Chronic Wounds
Bacteria by Products
Formulation
Quality Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12351
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12351
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activitySesto Cabral, María EugeniaRamos Vernieri, Alberto NicolásMacedo, Alexandre Jose Trentin, DanielleTreter, YaninneManzo, Ruben HilarioValdez, Juan CarlosChronic WoundsBacteria by ProductsFormulationQuality Controlhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Chronic wounds are those that remain in a chronic inflammatory state and fail to follow normal healing process. Infection is one of the most important causes of chronicity. A frequent pathogen isolated from chronic infections is Pseudomonas aeruginosa; refractory to therapy and host immune attack in its biofilm phenotype. Lactobacillus plantarum cultures supernatants (LAPS) interfere with its pathogenic capacity. In addition, LAPS showed bacteriostatic and bactericide properties and is neither cytotoxic nor an inductor of necrosis-apoptosis. LAPSs chemical composition was determined; allowing us to propose a correlation between its constituents and their biological activity. This article shows a pharmaceutical dosage form designed by using LAPS as an API with pro-healing activity and its quality control. Pharmacotechnical and anti-microbial assays were adapted to demonstrate that the vehicle used does not modify LAPS activities. Selected formulation (F100) showed fair mechanical and technological properties. From the in vitro release assays was found an adequate release from the carrier matrix and maintains its anti-pathogenic activity for 6 months. We propose F100 for chronic wounds treatment. The use of harmless bacteria by-products, such as LAPS, to antagonize infectious pathogens that have ability to form biofilm is an efficient and economic approach to treat infected chronic wounds.Fil: Sesto Cabral, María Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ramos Vernieri, Alberto Nicolás. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Macedo, Alexandre Jose . Universidade Federal Do Rio Grande Do Sul; BrasilFil: Trentin, Danielle. Universidade Federal Do Rio Grande Do Sul; BrasilFil: Treter, Yaninne. Universidade Federal Do Rio Grande Do Sul; BrasilFil: Manzo, Ruben Hilario. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valdez, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12351Sesto Cabral, María Eugenia; Ramos Vernieri, Alberto Nicolás; Macedo, Alexandre Jose ; Trentin, Danielle; Treter, Yaninne; et al.; Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity; Taylor & Francis; Pharmaceutical Development And Technology; 20; 8; -1-2015; 911-9181083-74501097-9867enginfo:eu-repo/semantics/altIdentifier/doi/10.3109/10837450.2014.938858info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10837450.2014.938858info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:53Zoai:ri.conicet.gov.ar:11336/12351instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:53.579CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
title Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
spellingShingle Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
Sesto Cabral, María Eugenia
Chronic Wounds
Bacteria by Products
Formulation
Quality Control
title_short Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
title_full Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
title_fullStr Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
title_full_unstemmed Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
title_sort Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
dc.creator.none.fl_str_mv Sesto Cabral, María Eugenia
Ramos Vernieri, Alberto Nicolás
Macedo, Alexandre Jose
Trentin, Danielle
Treter, Yaninne
Manzo, Ruben Hilario
Valdez, Juan Carlos
author Sesto Cabral, María Eugenia
author_facet Sesto Cabral, María Eugenia
Ramos Vernieri, Alberto Nicolás
Macedo, Alexandre Jose
Trentin, Danielle
Treter, Yaninne
Manzo, Ruben Hilario
Valdez, Juan Carlos
author_role author
author2 Ramos Vernieri, Alberto Nicolás
Macedo, Alexandre Jose
Trentin, Danielle
Treter, Yaninne
Manzo, Ruben Hilario
Valdez, Juan Carlos
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chronic Wounds
Bacteria by Products
Formulation
Quality Control
topic Chronic Wounds
Bacteria by Products
Formulation
Quality Control
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chronic wounds are those that remain in a chronic inflammatory state and fail to follow normal healing process. Infection is one of the most important causes of chronicity. A frequent pathogen isolated from chronic infections is Pseudomonas aeruginosa; refractory to therapy and host immune attack in its biofilm phenotype. Lactobacillus plantarum cultures supernatants (LAPS) interfere with its pathogenic capacity. In addition, LAPS showed bacteriostatic and bactericide properties and is neither cytotoxic nor an inductor of necrosis-apoptosis. LAPSs chemical composition was determined; allowing us to propose a correlation between its constituents and their biological activity. This article shows a pharmaceutical dosage form designed by using LAPS as an API with pro-healing activity and its quality control. Pharmacotechnical and anti-microbial assays were adapted to demonstrate that the vehicle used does not modify LAPS activities. Selected formulation (F100) showed fair mechanical and technological properties. From the in vitro release assays was found an adequate release from the carrier matrix and maintains its anti-pathogenic activity for 6 months. We propose F100 for chronic wounds treatment. The use of harmless bacteria by-products, such as LAPS, to antagonize infectious pathogens that have ability to form biofilm is an efficient and economic approach to treat infected chronic wounds.
Fil: Sesto Cabral, María Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ramos Vernieri, Alberto Nicolás. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Macedo, Alexandre Jose . Universidade Federal Do Rio Grande Do Sul; Brasil
Fil: Trentin, Danielle. Universidade Federal Do Rio Grande Do Sul; Brasil
Fil: Treter, Yaninne. Universidade Federal Do Rio Grande Do Sul; Brasil
Fil: Manzo, Ruben Hilario. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valdez, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Chronic wounds are those that remain in a chronic inflammatory state and fail to follow normal healing process. Infection is one of the most important causes of chronicity. A frequent pathogen isolated from chronic infections is Pseudomonas aeruginosa; refractory to therapy and host immune attack in its biofilm phenotype. Lactobacillus plantarum cultures supernatants (LAPS) interfere with its pathogenic capacity. In addition, LAPS showed bacteriostatic and bactericide properties and is neither cytotoxic nor an inductor of necrosis-apoptosis. LAPSs chemical composition was determined; allowing us to propose a correlation between its constituents and their biological activity. This article shows a pharmaceutical dosage form designed by using LAPS as an API with pro-healing activity and its quality control. Pharmacotechnical and anti-microbial assays were adapted to demonstrate that the vehicle used does not modify LAPS activities. Selected formulation (F100) showed fair mechanical and technological properties. From the in vitro release assays was found an adequate release from the carrier matrix and maintains its anti-pathogenic activity for 6 months. We propose F100 for chronic wounds treatment. The use of harmless bacteria by-products, such as LAPS, to antagonize infectious pathogens that have ability to form biofilm is an efficient and economic approach to treat infected chronic wounds.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12351
Sesto Cabral, María Eugenia; Ramos Vernieri, Alberto Nicolás; Macedo, Alexandre Jose ; Trentin, Danielle; Treter, Yaninne; et al.; Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity; Taylor & Francis; Pharmaceutical Development And Technology; 20; 8; -1-2015; 911-918
1083-7450
1097-9867
url http://hdl.handle.net/11336/12351
identifier_str_mv Sesto Cabral, María Eugenia; Ramos Vernieri, Alberto Nicolás; Macedo, Alexandre Jose ; Trentin, Danielle; Treter, Yaninne; et al.; Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity; Taylor & Francis; Pharmaceutical Development And Technology; 20; 8; -1-2015; 911-918
1083-7450
1097-9867
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/10837450.2014.938858
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10837450.2014.938858
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083267059515392
score 13.22299

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