Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol

Autores
Cabezas, Dario Marcelino; Diehl, Bernd W. K.; Tomás, Mabel Cristina
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lecithins are widely used in the food industry because of their multifunctional characteristics. Fractionation of the original mixture of phospholipids in lecithin is desirable for certain applications. The influence of ethanol/water mixtures (90 : 10 to 96 : 4) and other operative conditions (temperature 35-65 °C, incubation time 30-90 min, solvent/lecithin ratio 2 : 1, 3 : 1) on the extraction of phosphatidylcholine (PC)-enriched fractions of sunflower lecithin (a non-GMO product) was investigated. Yield % and phospholipid composition of the enriched PC fractions as well as the residue were determined. The percent extraction coefficient of each phospholipid (EPC, EPE and EPI) in the enriched PC fraction was calculated. Values of EPC varied from 6.5 (35 °C, 30 min, 2 : 1, 90 : 10) to 52.6 (65 °C, 90 min, 3 : 1, 96 : 4). High temperature and long incubation time produced a significant increase of this coefficient (p <0.05) while a high water content in the ethanolic mixture resulted in a considerable decrease in PC extraction. EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC-enriched fractions at laboratory scale.
Fil: Cabezas, Dario Marcelino. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Diehl, Bernd W. K.. Laboratorium Für Auftragsanalytik; Alemania
Fil: Tomás, Mabel Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Materia
31P NMR
AQUEOUS ETHANOL FRACTIONATION
PHOSPHATIDYLCHOLINE
PHOSPHOLIPIDS
SUNFLOWER LECITHIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160311
Acceder
id CONICETDig_2450505f0b4c335fbd4406304d549f5d
oai_identifier_str oai:ri.conicet.gov.ar:11336/160311
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanolCabezas, Dario MarcelinoDiehl, Bernd W. K.Tomás, Mabel Cristina31P NMRAQUEOUS ETHANOL FRACTIONATIONPHOSPHATIDYLCHOLINEPHOSPHOLIPIDSSUNFLOWER LECITHINhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Lecithins are widely used in the food industry because of their multifunctional characteristics. Fractionation of the original mixture of phospholipids in lecithin is desirable for certain applications. The influence of ethanol/water mixtures (90 : 10 to 96 : 4) and other operative conditions (temperature 35-65 °C, incubation time 30-90 min, solvent/lecithin ratio 2 : 1, 3 : 1) on the extraction of phosphatidylcholine (PC)-enriched fractions of sunflower lecithin (a non-GMO product) was investigated. Yield % and phospholipid composition of the enriched PC fractions as well as the residue were determined. The percent extraction coefficient of each phospholipid (EPC, EPE and EPI) in the enriched PC fraction was calculated. Values of EPC varied from 6.5 (35 °C, 30 min, 2 : 1, 90 : 10) to 52.6 (65 °C, 90 min, 3 : 1, 96 : 4). High temperature and long incubation time produced a significant increase of this coefficient (p <0.05) while a high water content in the ethanolic mixture resulted in a considerable decrease in PC extraction. EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC-enriched fractions at laboratory scale.Fil: Cabezas, Dario Marcelino. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Diehl, Bernd W. K.. Laboratorium Für Auftragsanalytik; AlemaniaFil: Tomás, Mabel Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaWiley VCH Verlag2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160311Cabezas, Dario Marcelino; Diehl, Bernd W. K.; Tomás, Mabel Cristina; Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol; Wiley VCH Verlag; European Journal of Lipid Science and Technology; 111; 10; 10-2009; 993-10021438-76971438-9312CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ejlt.200900001info:eu-repo/semantics/altIdentifier/doi/10.1002/ejlt.200900001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:33Zoai:ri.conicet.gov.ar:11336/160311instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:33.429CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
title Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
spellingShingle Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
Cabezas, Dario Marcelino
31P NMR
AQUEOUS ETHANOL FRACTIONATION
PHOSPHATIDYLCHOLINE
PHOSPHOLIPIDS
SUNFLOWER LECITHIN
title_short Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
title_full Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
title_fullStr Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
title_full_unstemmed Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
title_sort Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol
dc.creator.none.fl_str_mv Cabezas, Dario Marcelino
Diehl, Bernd W. K.
Tomás, Mabel Cristina
author Cabezas, Dario Marcelino
author_facet Cabezas, Dario Marcelino
Diehl, Bernd W. K.
Tomás, Mabel Cristina
author_role author
author2 Diehl, Bernd W. K.
Tomás, Mabel Cristina
author2_role author
author
dc.subject.none.fl_str_mv 31P NMR
AQUEOUS ETHANOL FRACTIONATION
PHOSPHATIDYLCHOLINE
PHOSPHOLIPIDS
SUNFLOWER LECITHIN
topic 31P NMR
AQUEOUS ETHANOL FRACTIONATION
PHOSPHATIDYLCHOLINE
PHOSPHOLIPIDS
SUNFLOWER LECITHIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lecithins are widely used in the food industry because of their multifunctional characteristics. Fractionation of the original mixture of phospholipids in lecithin is desirable for certain applications. The influence of ethanol/water mixtures (90 : 10 to 96 : 4) and other operative conditions (temperature 35-65 °C, incubation time 30-90 min, solvent/lecithin ratio 2 : 1, 3 : 1) on the extraction of phosphatidylcholine (PC)-enriched fractions of sunflower lecithin (a non-GMO product) was investigated. Yield % and phospholipid composition of the enriched PC fractions as well as the residue were determined. The percent extraction coefficient of each phospholipid (EPC, EPE and EPI) in the enriched PC fraction was calculated. Values of EPC varied from 6.5 (35 °C, 30 min, 2 : 1, 90 : 10) to 52.6 (65 °C, 90 min, 3 : 1, 96 : 4). High temperature and long incubation time produced a significant increase of this coefficient (p <0.05) while a high water content in the ethanolic mixture resulted in a considerable decrease in PC extraction. EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC-enriched fractions at laboratory scale.
Fil: Cabezas, Dario Marcelino. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Diehl, Bernd W. K.. Laboratorium Für Auftragsanalytik; Alemania
Fil: Tomás, Mabel Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
description Lecithins are widely used in the food industry because of their multifunctional characteristics. Fractionation of the original mixture of phospholipids in lecithin is desirable for certain applications. The influence of ethanol/water mixtures (90 : 10 to 96 : 4) and other operative conditions (temperature 35-65 °C, incubation time 30-90 min, solvent/lecithin ratio 2 : 1, 3 : 1) on the extraction of phosphatidylcholine (PC)-enriched fractions of sunflower lecithin (a non-GMO product) was investigated. Yield % and phospholipid composition of the enriched PC fractions as well as the residue were determined. The percent extraction coefficient of each phospholipid (EPC, EPE and EPI) in the enriched PC fraction was calculated. Values of EPC varied from 6.5 (35 °C, 30 min, 2 : 1, 90 : 10) to 52.6 (65 °C, 90 min, 3 : 1, 96 : 4). High temperature and long incubation time produced a significant increase of this coefficient (p <0.05) while a high water content in the ethanolic mixture resulted in a considerable decrease in PC extraction. EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC-enriched fractions at laboratory scale.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160311
Cabezas, Dario Marcelino; Diehl, Bernd W. K.; Tomás, Mabel Cristina; Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol; Wiley VCH Verlag; European Journal of Lipid Science and Technology; 111; 10; 10-2009; 993-1002
1438-7697
1438-9312
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160311
identifier_str_mv Cabezas, Dario Marcelino; Diehl, Bernd W. K.; Tomás, Mabel Cristina; Effect of processing parameters on sunflower phosphatidylcholine-enriched fractions extracted with aqueous ethanol; Wiley VCH Verlag; European Journal of Lipid Science and Technology; 111; 10; 10-2009; 993-1002
1438-7697
1438-9312
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ejlt.200900001
info:eu-repo/semantics/altIdentifier/doi/10.1002/ejlt.200900001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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