Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems

Autores
Masullo, Luciano Andrés; Bodén, Andreas; Pennacchietti, Francesca; Coceano, Giovanna; Ratz, Michael; Testa, Ilaria
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45–65 nm within the entire cell volume at low light intensities (W-kW cm−2). Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON–OFF switching cycles and image contrast. By maximizing the detected photon flux, MoNaLISA enables prolonged (40–50 frames) and large (50 × 50 µm2) recordings at 0.3–1.3 Hz with enhanced optical sectioning ability. We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues.
Fil: Masullo, Luciano Andrés. Royal Institute of Technology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Bodén, Andreas. Royal Institute of Technology; Suecia
Fil: Pennacchietti, Francesca. Royal Institute of Technology; Suecia
Fil: Coceano, Giovanna. Royal Institute of Technology; Suecia
Fil: Ratz, Michael. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Testa, Ilaria. Royal Institute of Technology; Suecia
Materia
NANOSCOPY
LIVING
CELLS
TIMELAPSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90375
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systemsMasullo, Luciano AndrésBodén, AndreasPennacchietti, FrancescaCoceano, GiovannaRatz, MichaelTesta, IlariaNANOSCOPYLIVINGCELLSTIMELAPSEhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45–65 nm within the entire cell volume at low light intensities (W-kW cm−2). Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON–OFF switching cycles and image contrast. By maximizing the detected photon flux, MoNaLISA enables prolonged (40–50 frames) and large (50 × 50 µm2) recordings at 0.3–1.3 Hz with enhanced optical sectioning ability. We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues.Fil: Masullo, Luciano Andrés. Royal Institute of Technology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Bodén, Andreas. Royal Institute of Technology; SueciaFil: Pennacchietti, Francesca. Royal Institute of Technology; SueciaFil: Coceano, Giovanna. Royal Institute of Technology; SueciaFil: Ratz, Michael. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Testa, Ilaria. Royal Institute of Technology; SueciaNature Publishing Group2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90375Masullo, Luciano Andrés; Bodén, Andreas; Pennacchietti, Francesca; Coceano, Giovanna; Ratz, Michael; et al.; Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-92041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-05799-winfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-018-05799-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:56Zoai:ri.conicet.gov.ar:11336/90375instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:57.082CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
spellingShingle Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
Masullo, Luciano Andrés
NANOSCOPY
LIVING
CELLS
TIMELAPSE
title_short Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_full Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_fullStr Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_full_unstemmed Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_sort Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
dc.creator.none.fl_str_mv Masullo, Luciano Andrés
Bodén, Andreas
Pennacchietti, Francesca
Coceano, Giovanna
Ratz, Michael
Testa, Ilaria
author Masullo, Luciano Andrés
author_facet Masullo, Luciano Andrés
Bodén, Andreas
Pennacchietti, Francesca
Coceano, Giovanna
Ratz, Michael
Testa, Ilaria
author_role author
author2 Bodén, Andreas
Pennacchietti, Francesca
Coceano, Giovanna
Ratz, Michael
Testa, Ilaria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv NANOSCOPY
LIVING
CELLS
TIMELAPSE
topic NANOSCOPY
LIVING
CELLS
TIMELAPSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45–65 nm within the entire cell volume at low light intensities (W-kW cm−2). Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON–OFF switching cycles and image contrast. By maximizing the detected photon flux, MoNaLISA enables prolonged (40–50 frames) and large (50 × 50 µm2) recordings at 0.3–1.3 Hz with enhanced optical sectioning ability. We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues.
Fil: Masullo, Luciano Andrés. Royal Institute of Technology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Bodén, Andreas. Royal Institute of Technology; Suecia
Fil: Pennacchietti, Francesca. Royal Institute of Technology; Suecia
Fil: Coceano, Giovanna. Royal Institute of Technology; Suecia
Fil: Ratz, Michael. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Testa, Ilaria. Royal Institute of Technology; Suecia
description The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45–65 nm within the entire cell volume at low light intensities (W-kW cm−2). Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON–OFF switching cycles and image contrast. By maximizing the detected photon flux, MoNaLISA enables prolonged (40–50 frames) and large (50 × 50 µm2) recordings at 0.3–1.3 Hz with enhanced optical sectioning ability. We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90375
Masullo, Luciano Andrés; Bodén, Andreas; Pennacchietti, Francesca; Coceano, Giovanna; Ratz, Michael; et al.; Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-9
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90375
identifier_str_mv Masullo, Luciano Andrés; Bodén, Andreas; Pennacchietti, Francesca; Coceano, Giovanna; Ratz, Michael; et al.; Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-9
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-05799-w
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-018-05799-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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