Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.

Autores
Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Avagnina Iribarren, Maria Alejandra; Elsner, Boris; Monserrat, Alberto Juan
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Vasospasm could be due to an increase in tromboxane A2-mediated vasoconstriction or to a decrease in prostacyclin-induced vasodilatation. Enzymes involved in the synthesis of both compounds are tromboxane A2- and prostacyclin-synthase respectively. The aim of this study was to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the occurrence of VNTR in those choline-deficient rats which die because of acute renal failure and those which do not. We verified the presence of the VNTR in the prostacyclin synthase rat gene, but we did not find any difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in some rats is not related with differences in VNTR in the promoter region of the prostacyclin synthase gene. Weanling Sprague-Dawley rats fed a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Its pathogenesis is controversial. The ischemic mechanism has been proposed. Derivatives from arachidonic acid are involved in the regulation of vascular tone. Vasospasm could be due to an increase in vasoconstriction mediated by tromboxane A2 or a decrease in vasodilatation by prostacyclin. Enzymes involved in the synthesis of them are tromboxane A2 and prostacyclin synthase respectively. The aim of this study is to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the VNTR and those rats which dye as a consequence of acute renal failure due to choline deficiency and those which do not die. The VNTR presence was detected by molecular methods. We verified the presence of the VNTR in the prostacyclin synthase rat gene. We did not find difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in Sprague-Dawley rats is not related with differences in prostacyclin synthase VNTR, in the promoter region of this gene.
Fil: Denninghoff, Valeria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ossani, Georgina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Uceda, Ana Margarita. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Avagnina Iribarren, Maria Alejandra. Centro de Educaciones Médicas e Investigación Clínica "Norberto Quirno"; Argentina
Fil: Elsner, Boris. Centro de Educaciones Médicas e Investigación Clínica "Norberto Quirno"; Argentina
Fil: Monserrat, Alberto Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Materia
VNTR
PROSTACYCLIN SYNTHASE GENE
KIDNEY
CHOLINE DEFICIENCY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112991
Acceder
id CONICETDig_23eae728b69a7c4226918856891d23b9
oai_identifier_str oai:ri.conicet.gov.ar:11336/112991
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.Denninghoff, Valeria CeciliaOssani, Georgina PaulaUceda, Ana MargaritaAvagnina Iribarren, Maria AlejandraElsner, BorisMonserrat, Alberto JuanVNTRPROSTACYCLIN SYNTHASE GENEKIDNEYCHOLINE DEFICIENCYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Vasospasm could be due to an increase in tromboxane A2-mediated vasoconstriction or to a decrease in prostacyclin-induced vasodilatation. Enzymes involved in the synthesis of both compounds are tromboxane A2- and prostacyclin-synthase respectively. The aim of this study was to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the occurrence of VNTR in those choline-deficient rats which die because of acute renal failure and those which do not. We verified the presence of the VNTR in the prostacyclin synthase rat gene, but we did not find any difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in some rats is not related with differences in VNTR in the promoter region of the prostacyclin synthase gene. Weanling Sprague-Dawley rats fed a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Its pathogenesis is controversial. The ischemic mechanism has been proposed. Derivatives from arachidonic acid are involved in the regulation of vascular tone. Vasospasm could be due to an increase in vasoconstriction mediated by tromboxane A2 or a decrease in vasodilatation by prostacyclin. Enzymes involved in the synthesis of them are tromboxane A2 and prostacyclin synthase respectively. The aim of this study is to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the VNTR and those rats which dye as a consequence of acute renal failure due to choline deficiency and those which do not die. The VNTR presence was detected by molecular methods. We verified the presence of the VNTR in the prostacyclin synthase rat gene. We did not find difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in Sprague-Dawley rats is not related with differences in prostacyclin synthase VNTR, in the promoter region of this gene.Fil: Denninghoff, Valeria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ossani, Georgina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; ArgentinaFil: Uceda, Ana Margarita. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; ArgentinaFil: Avagnina Iribarren, Maria Alejandra. Centro de Educaciones Médicas e Investigación Clínica "Norberto Quirno"; ArgentinaFil: Elsner, Boris. Centro de Educaciones Médicas e Investigación Clínica "Norberto Quirno"; ArgentinaFil: Monserrat, Alberto Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; ArgentinaInstituto de Histología y Embriología2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112991Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Avagnina Iribarren, Maria Alejandra; Elsner, Boris; et al.; Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.; Instituto de Histología y Embriología; Biocell; 34; 2; 7-2010; 65-700327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:10Zoai:ri.conicet.gov.ar:11336/112991instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:10.962CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
title Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
spellingShingle Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
Denninghoff, Valeria Cecilia
VNTR
PROSTACYCLIN SYNTHASE GENE
KIDNEY
CHOLINE DEFICIENCY
title_short Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
title_full Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
title_fullStr Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
title_full_unstemmed Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
title_sort Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.
dc.creator.none.fl_str_mv Denninghoff, Valeria Cecilia
Ossani, Georgina Paula
Uceda, Ana Margarita
Avagnina Iribarren, Maria Alejandra
Elsner, Boris
Monserrat, Alberto Juan
author Denninghoff, Valeria Cecilia
author_facet Denninghoff, Valeria Cecilia
Ossani, Georgina Paula
Uceda, Ana Margarita
Avagnina Iribarren, Maria Alejandra
Elsner, Boris
Monserrat, Alberto Juan
author_role author
author2 Ossani, Georgina Paula
Uceda, Ana Margarita
Avagnina Iribarren, Maria Alejandra
Elsner, Boris
Monserrat, Alberto Juan
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv VNTR
PROSTACYCLIN SYNTHASE GENE
KIDNEY
CHOLINE DEFICIENCY
topic VNTR
PROSTACYCLIN SYNTHASE GENE
KIDNEY
CHOLINE DEFICIENCY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Vasospasm could be due to an increase in tromboxane A2-mediated vasoconstriction or to a decrease in prostacyclin-induced vasodilatation. Enzymes involved in the synthesis of both compounds are tromboxane A2- and prostacyclin-synthase respectively. The aim of this study was to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the occurrence of VNTR in those choline-deficient rats which die because of acute renal failure and those which do not. We verified the presence of the VNTR in the prostacyclin synthase rat gene, but we did not find any difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in some rats is not related with differences in VNTR in the promoter region of the prostacyclin synthase gene. Weanling Sprague-Dawley rats fed a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Its pathogenesis is controversial. The ischemic mechanism has been proposed. Derivatives from arachidonic acid are involved in the regulation of vascular tone. Vasospasm could be due to an increase in vasoconstriction mediated by tromboxane A2 or a decrease in vasodilatation by prostacyclin. Enzymes involved in the synthesis of them are tromboxane A2 and prostacyclin synthase respectively. The aim of this study is to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the VNTR and those rats which dye as a consequence of acute renal failure due to choline deficiency and those which do not die. The VNTR presence was detected by molecular methods. We verified the presence of the VNTR in the prostacyclin synthase rat gene. We did not find difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in Sprague-Dawley rats is not related with differences in prostacyclin synthase VNTR, in the promoter region of this gene.
Fil: Denninghoff, Valeria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ossani, Georgina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Uceda, Ana Margarita. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Avagnina Iribarren, Maria Alejandra. Centro de Educaciones Médicas e Investigación Clínica "Norberto Quirno"; Argentina
Fil: Elsner, Boris. Centro de Educaciones Médicas e Investigación Clínica "Norberto Quirno"; Argentina
Fil: Monserrat, Alberto Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
description Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Vasospasm could be due to an increase in tromboxane A2-mediated vasoconstriction or to a decrease in prostacyclin-induced vasodilatation. Enzymes involved in the synthesis of both compounds are tromboxane A2- and prostacyclin-synthase respectively. The aim of this study was to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the occurrence of VNTR in those choline-deficient rats which die because of acute renal failure and those which do not. We verified the presence of the VNTR in the prostacyclin synthase rat gene, but we did not find any difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in some rats is not related with differences in VNTR in the promoter region of the prostacyclin synthase gene. Weanling Sprague-Dawley rats fed a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Its pathogenesis is controversial. The ischemic mechanism has been proposed. Derivatives from arachidonic acid are involved in the regulation of vascular tone. Vasospasm could be due to an increase in vasoconstriction mediated by tromboxane A2 or a decrease in vasodilatation by prostacyclin. Enzymes involved in the synthesis of them are tromboxane A2 and prostacyclin synthase respectively. The aim of this study is to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the VNTR and those rats which dye as a consequence of acute renal failure due to choline deficiency and those which do not die. The VNTR presence was detected by molecular methods. We verified the presence of the VNTR in the prostacyclin synthase rat gene. We did not find difference in the molecular weight of the alleles between experimental and control rats. Renal reparation of the acute kidney injury due to choline deficiency in Sprague-Dawley rats is not related with differences in prostacyclin synthase VNTR, in the promoter region of this gene.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112991
Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Avagnina Iribarren, Maria Alejandra; Elsner, Boris; et al.; Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.; Instituto de Histología y Embriología; Biocell; 34; 2; 7-2010; 65-70
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112991
identifier_str_mv Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Avagnina Iribarren, Maria Alejandra; Elsner, Boris; et al.; Variable Number Tandem Repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.; Instituto de Histología y Embriología; Biocell; 34; 2; 7-2010; 65-70
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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