Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect

Autores
Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Rugnone, Matias Leandro; Fernandez, Elmer Andres; Fresno Rodríguez, Cristóbal; González, German; Díaz, Maria Luisa; Avagnina, Alejandra; Elsner, Boris; Monserrat, Alberto Juan
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model. Methods Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed. Results All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats. Conclusions This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.
Fil: Denninghoff, Valeria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ossani, Georgina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Uceda, Ana Margarita. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Rugnone, Matias Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Fernandez, Elmer Andres. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Fresno Rodríguez, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina
Fil: González, German. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina
Fil: Díaz, Maria Luisa. Hospital Británico de Buenos Aires; Argentina
Fil: Avagnina, Alejandra. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Elsner, Boris. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Monserrat, Alberto Juan. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Acute Kidney Injury
Choline-Deficient
Gene Expression
Menhaden Oil
Protective Effect
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81785
Acceder
id CONICETDig_0b4094dcc687d590578e00a05cde8d7b
oai_identifier_str oai:ri.conicet.gov.ar:11336/81785
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effectDenninghoff, Valeria CeciliaOssani, Georgina PaulaUceda, Ana MargaritaRugnone, Matias LeandroFernandez, Elmer AndresFresno Rodríguez, CristóbalGonzález, GermanDíaz, Maria LuisaAvagnina, AlejandraElsner, BorisMonserrat, Alberto JuanAcute Kidney InjuryCholine-DeficientGene ExpressionMenhaden OilProtective Effecthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model. Methods Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed. Results All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats. Conclusions This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.Fil: Denninghoff, Valeria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ossani, Georgina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; ArgentinaFil: Uceda, Ana Margarita. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; ArgentinaFil: Rugnone, Matias Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Fernandez, Elmer Andres. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Fresno Rodríguez, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; ArgentinaFil: González, German. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; ArgentinaFil: Díaz, Maria Luisa. Hospital Británico de Buenos Aires; ArgentinaFil: Avagnina, Alejandra. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; ArgentinaFil: Elsner, Boris. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; ArgentinaFil: Monserrat, Alberto Juan. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaDr Dietrich Steinkopff Verlag2013-10-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81785Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Rugnone, Matias Leandro; Fernandez, Elmer Andres; et al.; Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect; Dr Dietrich Steinkopff Verlag; European Journal of Nutrition; 53; 3; 16-10-2013; 897-9061436-62071436-6215CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00394-013-0593-8info:eu-repo/semantics/altIdentifier/doi/10.1007/s00394-013-0593-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:57Zoai:ri.conicet.gov.ar:11336/81785instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:57.953CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
title Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
spellingShingle Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
Denninghoff, Valeria Cecilia
Acute Kidney Injury
Choline-Deficient
Gene Expression
Menhaden Oil
Protective Effect
title_short Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
title_full Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
title_fullStr Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
title_full_unstemmed Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
title_sort Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
dc.creator.none.fl_str_mv Denninghoff, Valeria Cecilia
Ossani, Georgina Paula
Uceda, Ana Margarita
Rugnone, Matias Leandro
Fernandez, Elmer Andres
Fresno Rodríguez, Cristóbal
González, German
Díaz, Maria Luisa
Avagnina, Alejandra
Elsner, Boris
Monserrat, Alberto Juan
author Denninghoff, Valeria Cecilia
author_facet Denninghoff, Valeria Cecilia
Ossani, Georgina Paula
Uceda, Ana Margarita
Rugnone, Matias Leandro
Fernandez, Elmer Andres
Fresno Rodríguez, Cristóbal
González, German
Díaz, Maria Luisa
Avagnina, Alejandra
Elsner, Boris
Monserrat, Alberto Juan
author_role author
author2 Ossani, Georgina Paula
Uceda, Ana Margarita
Rugnone, Matias Leandro
Fernandez, Elmer Andres
Fresno Rodríguez, Cristóbal
González, German
Díaz, Maria Luisa
Avagnina, Alejandra
Elsner, Boris
Monserrat, Alberto Juan
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Acute Kidney Injury
Choline-Deficient
Gene Expression
Menhaden Oil
Protective Effect
topic Acute Kidney Injury
Choline-Deficient
Gene Expression
Menhaden Oil
Protective Effect
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model. Methods Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed. Results All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats. Conclusions This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.
Fil: Denninghoff, Valeria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ossani, Georgina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Uceda, Ana Margarita. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Rugnone, Matias Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Fernandez, Elmer Andres. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Fresno Rodríguez, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina
Fil: González, German. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina
Fil: Díaz, Maria Luisa. Hospital Británico de Buenos Aires; Argentina
Fil: Avagnina, Alejandra. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Elsner, Boris. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Monserrat, Alberto Juan. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model. Methods Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed. Results All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats. Conclusions This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81785
Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Rugnone, Matias Leandro; Fernandez, Elmer Andres; et al.; Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect; Dr Dietrich Steinkopff Verlag; European Journal of Nutrition; 53; 3; 16-10-2013; 897-906
1436-6207
1436-6215
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81785
identifier_str_mv Denninghoff, Valeria Cecilia; Ossani, Georgina Paula; Uceda, Ana Margarita; Rugnone, Matias Leandro; Fernandez, Elmer Andres; et al.; Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect; Dr Dietrich Steinkopff Verlag; European Journal of Nutrition; 53; 3; 16-10-2013; 897-906
1436-6207
1436-6215
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00394-013-0593-8
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00394-013-0593-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Dr Dietrich Steinkopff Verlag
publisher.none.fl_str_mv Dr Dietrich Steinkopff Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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