Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay

Autores
Pallarola, Diego Andres; Battaglini, Fernando
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. The method presents several advantages: it is carried out in a mild environment, good conjugation ratios are obtained, it is suitable for any label bearing amino, hydrazine, or hydrazide groups, and the LPS endotoxic and HRP enzymatic activities are preserved. The HRP conjugate is applied in an amperometric competitive assay for the detection of lipopolysaccharides in an electrode array combined with a multipotentiostat able to carry out simultaneous determinations. The system is able to detect samples in concentrations as low as 100 pg mL-1 of LPS.
Fil: Pallarola, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Battaglini, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
Lipopolysaccharide Conjugates
Lipopolysaccharide Detection
1-Cyano-4-Dimethylaminopyridinium Tetrafluoroborate
Horseradish Peroxidase
Dansyl Hydrazine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80450
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/80450
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assayPallarola, Diego AndresBattaglini, FernandoLipopolysaccharide ConjugatesLipopolysaccharide Detection1-Cyano-4-Dimethylaminopyridinium TetrafluoroborateHorseradish PeroxidaseDansyl Hydrazinehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. The method presents several advantages: it is carried out in a mild environment, good conjugation ratios are obtained, it is suitable for any label bearing amino, hydrazine, or hydrazide groups, and the LPS endotoxic and HRP enzymatic activities are preserved. The HRP conjugate is applied in an amperometric competitive assay for the detection of lipopolysaccharides in an electrode array combined with a multipotentiostat able to carry out simultaneous determinations. The system is able to detect samples in concentrations as low as 100 pg mL-1 of LPS.Fil: Pallarola, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Battaglini, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaAmerican Chemical Society2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80450Pallarola, Diego Andres; Battaglini, Fernando; Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay; American Chemical Society; Analytical Chemistry; 81; 10; 5-2009; 3824-38290003-2700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac9001639info:eu-repo/semantics/altIdentifier/doi/10.1021/ac9001639info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:22Zoai:ri.conicet.gov.ar:11336/80450instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:23.027CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
title Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
spellingShingle Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
Pallarola, Diego Andres
Lipopolysaccharide Conjugates
Lipopolysaccharide Detection
1-Cyano-4-Dimethylaminopyridinium Tetrafluoroborate
Horseradish Peroxidase
Dansyl Hydrazine
title_short Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
title_full Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
title_fullStr Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
title_full_unstemmed Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
title_sort Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
dc.creator.none.fl_str_mv Pallarola, Diego Andres
Battaglini, Fernando
author Pallarola, Diego Andres
author_facet Pallarola, Diego Andres
Battaglini, Fernando
author_role author
author2 Battaglini, Fernando
author2_role author
dc.subject.none.fl_str_mv Lipopolysaccharide Conjugates
Lipopolysaccharide Detection
1-Cyano-4-Dimethylaminopyridinium Tetrafluoroborate
Horseradish Peroxidase
Dansyl Hydrazine
topic Lipopolysaccharide Conjugates
Lipopolysaccharide Detection
1-Cyano-4-Dimethylaminopyridinium Tetrafluoroborate
Horseradish Peroxidase
Dansyl Hydrazine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. The method presents several advantages: it is carried out in a mild environment, good conjugation ratios are obtained, it is suitable for any label bearing amino, hydrazine, or hydrazide groups, and the LPS endotoxic and HRP enzymatic activities are preserved. The HRP conjugate is applied in an amperometric competitive assay for the detection of lipopolysaccharides in an electrode array combined with a multipotentiostat able to carry out simultaneous determinations. The system is able to detect samples in concentrations as low as 100 pg mL-1 of LPS.
Fil: Pallarola, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Battaglini, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. The method presents several advantages: it is carried out in a mild environment, good conjugation ratios are obtained, it is suitable for any label bearing amino, hydrazine, or hydrazide groups, and the LPS endotoxic and HRP enzymatic activities are preserved. The HRP conjugate is applied in an amperometric competitive assay for the detection of lipopolysaccharides in an electrode array combined with a multipotentiostat able to carry out simultaneous determinations. The system is able to detect samples in concentrations as low as 100 pg mL-1 of LPS.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80450
Pallarola, Diego Andres; Battaglini, Fernando; Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay; American Chemical Society; Analytical Chemistry; 81; 10; 5-2009; 3824-3829
0003-2700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80450
identifier_str_mv Pallarola, Diego Andres; Battaglini, Fernando; Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay; American Chemical Society; Analytical Chemistry; 81; 10; 5-2009; 3824-3829
0003-2700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac9001639
info:eu-repo/semantics/altIdentifier/doi/10.1021/ac9001639
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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