In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)

Autores
Magnoni, Leonardo Julián; Roher, Nerea; Crespo, Diego; Krasnov, Aleksei; Planas, Josep V.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout.
Fil: Magnoni, Leonardo Julián. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
Fil: Crespo, Diego. Universidad de Barcelona; España
Fil: Krasnov, Aleksei. Nofima; Noruega
Fil: Planas, Josep V.. Universidad de Barcelona; España
Materia
FISH
LIPOPOLYSACCHARIDE
MUSCLE
PROTEOME
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38177
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38177
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)Magnoni, Leonardo JuliánRoher, NereaCrespo, DiegoKrasnov, AlekseiPlanas, Josep V.FISHLIPOPOLYSACCHARIDEMUSCLEPROTEOMETRANSCRIPTOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout.Fil: Magnoni, Leonardo Julián. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roher, Nerea. Universitat Autònoma de Barcelona; EspañaFil: Crespo, Diego. Universidad de Barcelona; EspañaFil: Krasnov, Aleksei. Nofima; NoruegaFil: Planas, Josep V.. Universidad de Barcelona; EspañaMDPI AG2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38177Magnoni, Leonardo Julián; Roher, Nerea; Crespo, Diego; Krasnov, Aleksei; Planas, Josep V.; In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss); MDPI AG; Biology; 4; 1; 2-2015; 67-872079-7737CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/biology4010067info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2079-7737/4/1/67info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:44Zoai:ri.conicet.gov.ar:11336/38177instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:45.17CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
title In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
spellingShingle In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
Magnoni, Leonardo Julián
FISH
LIPOPOLYSACCHARIDE
MUSCLE
PROTEOME
TRANSCRIPTOME
title_short In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
title_full In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
title_fullStr In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
title_full_unstemmed In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
title_sort In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
dc.creator.none.fl_str_mv Magnoni, Leonardo Julián
Roher, Nerea
Crespo, Diego
Krasnov, Aleksei
Planas, Josep V.
author Magnoni, Leonardo Julián
author_facet Magnoni, Leonardo Julián
Roher, Nerea
Crespo, Diego
Krasnov, Aleksei
Planas, Josep V.
author_role author
author2 Roher, Nerea
Crespo, Diego
Krasnov, Aleksei
Planas, Josep V.
author2_role author
author
author
author
dc.subject.none.fl_str_mv FISH
LIPOPOLYSACCHARIDE
MUSCLE
PROTEOME
TRANSCRIPTOME
topic FISH
LIPOPOLYSACCHARIDE
MUSCLE
PROTEOME
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout.
Fil: Magnoni, Leonardo Julián. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
Fil: Crespo, Diego. Universidad de Barcelona; España
Fil: Krasnov, Aleksei. Nofima; Noruega
Fil: Planas, Josep V.. Universidad de Barcelona; España
description The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38177
Magnoni, Leonardo Julián; Roher, Nerea; Crespo, Diego; Krasnov, Aleksei; Planas, Josep V.; In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss); MDPI AG; Biology; 4; 1; 2-2015; 67-87
2079-7737
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38177
identifier_str_mv Magnoni, Leonardo Julián; Roher, Nerea; Crespo, Diego; Krasnov, Aleksei; Planas, Josep V.; In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss); MDPI AG; Biology; 4; 1; 2-2015; 67-87
2079-7737
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/biology4010067
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2079-7737/4/1/67
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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