The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14

Autores
Murofushi, Yo; Villena, Julio Cesar; Morie, Kyoko; Kanmani, Paulraj; Tohno, Masanori; Shimazu, Tomoyuki; Aso, Hisashi; Suda, Yoshihito; Hashiguchi, Kenji; Saito, Tadao; Kitazawa, Haruki
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The radioprotective 105 (RP105)/MD1 complex is a member of the Toll-like receptor (TLR) family. It was reported that RP105/MD1 cooperates with the lipopolysaccharide (LPS) receptor TLR4/MD2 complex and plays a crucial role in the response of immune cells to LPS. This work evaluated whether RP105, TLR4 or TLR2 were involved in the immunoregulatory capacities of Lactobacillus plantarum N14 (LP14) or its exopolysaccharides (EPS). EPS from LP14 were fractionated into neutral (NPS) and acidic (APS) EPS by anion exchange chromatography. Experiments with transfectant HEKRP105/MD1 and HEKTLR2 cells demonstrated that LP14 strongly activated NF-κB via RP105 and TLR2. When we studied the capacity of APS to activate NF-κB pathway in HEKRP105/MD1 and HEKTLR4 cells; we observed that APS strongly stimulated both transfectant cells. Our results also showed that LP14 and APS were able to decrease the production of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) in porcine intestinal epithelial (PIE) cells in response to enterotoxigenic Escherichia coli (ETEC) challenge. In order to confirm the role of TLR2, TLR4 and RP105 in the immunoregulatory effect of APS from LP14, we used small interfering RNA (siRNA) to knockdown these receptors in PIE cells. The capacity of LP14 and APS to modulate pro-inflammatory cytokine expression was significantly reduced in PIERP105-/- cells. It was also shown that LP14 and APS were capable of upregulating negative regulators of the TLR signaling in PIE cells. This work describes for the first time that a Lactobacillus strain and its EPS reduce inflammation in intestinal epithelial cells in a RP105/MD1-dependend manner.
Fil: Murofushi, Yo. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Morie, Kyoko. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Tohno, Masanori. National Agricultural Research Organization. National Institute of Livestock and Grassland Science; Japón
Fil: Shimazu, Tomoyuki. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Hashiguchi, Kenji. Momoya. Research Center; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
EXOPOLYSACCHARIDES
LACTOBACILLUS PLANTARUM N14
PIE CELLS
RP105/MD1
TLR2
TLR4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2773
Acceder
id CONICETDig_237d888773cb0441dc4f0fe5428245b8
oai_identifier_str oai:ri.conicet.gov.ar:11336/2773
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14Murofushi, YoVillena, Julio CesarMorie, KyokoKanmani, PaulrajTohno, MasanoriShimazu, TomoyukiAso, HisashiSuda, YoshihitoHashiguchi, KenjiSaito, TadaoKitazawa, HarukiEXOPOLYSACCHARIDESLACTOBACILLUS PLANTARUM N14PIE CELLSRP105/MD1TLR2TLR4https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The radioprotective 105 (RP105)/MD1 complex is a member of the Toll-like receptor (TLR) family. It was reported that RP105/MD1 cooperates with the lipopolysaccharide (LPS) receptor TLR4/MD2 complex and plays a crucial role in the response of immune cells to LPS. This work evaluated whether RP105, TLR4 or TLR2 were involved in the immunoregulatory capacities of Lactobacillus plantarum N14 (LP14) or its exopolysaccharides (EPS). EPS from LP14 were fractionated into neutral (NPS) and acidic (APS) EPS by anion exchange chromatography. Experiments with transfectant HEKRP105/MD1 and HEKTLR2 cells demonstrated that LP14 strongly activated NF-κB via RP105 and TLR2. When we studied the capacity of APS to activate NF-κB pathway in HEKRP105/MD1 and HEKTLR4 cells; we observed that APS strongly stimulated both transfectant cells. Our results also showed that LP14 and APS were able to decrease the production of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) in porcine intestinal epithelial (PIE) cells in response to enterotoxigenic Escherichia coli (ETEC) challenge. In order to confirm the role of TLR2, TLR4 and RP105 in the immunoregulatory effect of APS from LP14, we used small interfering RNA (siRNA) to knockdown these receptors in PIE cells. The capacity of LP14 and APS to modulate pro-inflammatory cytokine expression was significantly reduced in PIERP105-/- cells. It was also shown that LP14 and APS were capable of upregulating negative regulators of the TLR signaling in PIE cells. This work describes for the first time that a Lactobacillus strain and its EPS reduce inflammation in intestinal epithelial cells in a RP105/MD1-dependend manner.Fil: Murofushi, Yo. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Morie, Kyoko. Tohoku University; JapónFil: Kanmani, Paulraj. Tohoku University; JapónFil: Tohno, Masanori. National Agricultural Research Organization. National Institute of Livestock and Grassland Science; JapónFil: Shimazu, Tomoyuki. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Hashiguchi, Kenji. Momoya. Research Center; JapónFil: Saito, Tadao. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónPergamon-Elsevier Science Ltd2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2773Murofushi, Yo; Villena, Julio Cesar; Morie, Kyoko; Kanmani, Paulraj; Tohno, Masanori; et al.; The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14; Pergamon-Elsevier Science Ltd; Molecular Immunology; 64; 1; 3-2015; 63-750161-58901872-9142enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2014.10.027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:30:29Zoai:ri.conicet.gov.ar:11336/2773instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:30:30.218CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
title The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
spellingShingle The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
Murofushi, Yo
EXOPOLYSACCHARIDES
LACTOBACILLUS PLANTARUM N14
PIE CELLS
RP105/MD1
TLR2
TLR4
title_short The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
title_full The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
title_fullStr The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
title_full_unstemmed The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
title_sort The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
dc.creator.none.fl_str_mv Murofushi, Yo
Villena, Julio Cesar
Morie, Kyoko
Kanmani, Paulraj
Tohno, Masanori
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Hashiguchi, Kenji
Saito, Tadao
Kitazawa, Haruki
author Murofushi, Yo
author_facet Murofushi, Yo
Villena, Julio Cesar
Morie, Kyoko
Kanmani, Paulraj
Tohno, Masanori
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Hashiguchi, Kenji
Saito, Tadao
Kitazawa, Haruki
author_role author
author2 Villena, Julio Cesar
Morie, Kyoko
Kanmani, Paulraj
Tohno, Masanori
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Hashiguchi, Kenji
Saito, Tadao
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EXOPOLYSACCHARIDES
LACTOBACILLUS PLANTARUM N14
PIE CELLS
RP105/MD1
TLR2
TLR4
topic EXOPOLYSACCHARIDES
LACTOBACILLUS PLANTARUM N14
PIE CELLS
RP105/MD1
TLR2
TLR4
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The radioprotective 105 (RP105)/MD1 complex is a member of the Toll-like receptor (TLR) family. It was reported that RP105/MD1 cooperates with the lipopolysaccharide (LPS) receptor TLR4/MD2 complex and plays a crucial role in the response of immune cells to LPS. This work evaluated whether RP105, TLR4 or TLR2 were involved in the immunoregulatory capacities of Lactobacillus plantarum N14 (LP14) or its exopolysaccharides (EPS). EPS from LP14 were fractionated into neutral (NPS) and acidic (APS) EPS by anion exchange chromatography. Experiments with transfectant HEKRP105/MD1 and HEKTLR2 cells demonstrated that LP14 strongly activated NF-κB via RP105 and TLR2. When we studied the capacity of APS to activate NF-κB pathway in HEKRP105/MD1 and HEKTLR4 cells; we observed that APS strongly stimulated both transfectant cells. Our results also showed that LP14 and APS were able to decrease the production of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) in porcine intestinal epithelial (PIE) cells in response to enterotoxigenic Escherichia coli (ETEC) challenge. In order to confirm the role of TLR2, TLR4 and RP105 in the immunoregulatory effect of APS from LP14, we used small interfering RNA (siRNA) to knockdown these receptors in PIE cells. The capacity of LP14 and APS to modulate pro-inflammatory cytokine expression was significantly reduced in PIERP105-/- cells. It was also shown that LP14 and APS were capable of upregulating negative regulators of the TLR signaling in PIE cells. This work describes for the first time that a Lactobacillus strain and its EPS reduce inflammation in intestinal epithelial cells in a RP105/MD1-dependend manner.
Fil: Murofushi, Yo. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Morie, Kyoko. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Tohno, Masanori. National Agricultural Research Organization. National Institute of Livestock and Grassland Science; Japón
Fil: Shimazu, Tomoyuki. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Hashiguchi, Kenji. Momoya. Research Center; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description The radioprotective 105 (RP105)/MD1 complex is a member of the Toll-like receptor (TLR) family. It was reported that RP105/MD1 cooperates with the lipopolysaccharide (LPS) receptor TLR4/MD2 complex and plays a crucial role in the response of immune cells to LPS. This work evaluated whether RP105, TLR4 or TLR2 were involved in the immunoregulatory capacities of Lactobacillus plantarum N14 (LP14) or its exopolysaccharides (EPS). EPS from LP14 were fractionated into neutral (NPS) and acidic (APS) EPS by anion exchange chromatography. Experiments with transfectant HEKRP105/MD1 and HEKTLR2 cells demonstrated that LP14 strongly activated NF-κB via RP105 and TLR2. When we studied the capacity of APS to activate NF-κB pathway in HEKRP105/MD1 and HEKTLR4 cells; we observed that APS strongly stimulated both transfectant cells. Our results also showed that LP14 and APS were able to decrease the production of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) in porcine intestinal epithelial (PIE) cells in response to enterotoxigenic Escherichia coli (ETEC) challenge. In order to confirm the role of TLR2, TLR4 and RP105 in the immunoregulatory effect of APS from LP14, we used small interfering RNA (siRNA) to knockdown these receptors in PIE cells. The capacity of LP14 and APS to modulate pro-inflammatory cytokine expression was significantly reduced in PIERP105-/- cells. It was also shown that LP14 and APS were capable of upregulating negative regulators of the TLR signaling in PIE cells. This work describes for the first time that a Lactobacillus strain and its EPS reduce inflammation in intestinal epithelial cells in a RP105/MD1-dependend manner.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2773
Murofushi, Yo; Villena, Julio Cesar; Morie, Kyoko; Kanmani, Paulraj; Tohno, Masanori; et al.; The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14; Pergamon-Elsevier Science Ltd; Molecular Immunology; 64; 1; 3-2015; 63-75
0161-5890
1872-9142
url http://hdl.handle.net/11336/2773
identifier_str_mv Murofushi, Yo; Villena, Julio Cesar; Morie, Kyoko; Kanmani, Paulraj; Tohno, Masanori; et al.; The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14; Pergamon-Elsevier Science Ltd; Molecular Immunology; 64; 1; 3-2015; 63-75
0161-5890
1872-9142
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2014.10.027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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