Perinatal derivatives: How to best validate their immunomodulatory functions

Autores
Papait, Andrea; Silini, Antonietta Rosa; Gazouli, Maria; Malvicini, Ricardo; Muraca, Maurizio; O' Driscoll, Lorraine; Pacienza, Natalia Alejandra; Toh, Wei Seong; Yannarelli, Gustavo Gabriel; Ponsaerts, Peter; Parolini, Ornella; Eissner, Günther; Pozzobon, Michela; Lim, Sai Kiang; Giebel, Bernd
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Perinatal tissues, mainly the placenta and umbilical cord, contain a variety of different somatic stem and progenitor cell types, including those of the hematopoietic system, multipotent mesenchymal stromal cells (MSCs), epithelial cells and amnion epithelial cells. Several of these perinatal derivatives (PnDs), as well as their secreted products, have been reported to exert immunomodulatory therapeutic and regenerative functions in a variety of pre-clinical disease models. Following experience with MSCs and their extracellular vesicle (EV) products, successful clinical translation of PnDs will require robust functional assays that are predictive for the relevant therapeutic potency. Using the examples of T cell and monocyte/macrophage assays, we here discuss several assay relevant parameters for assessing the immunomodulatory activities of PnDs. Furthermore, we highlight the need to correlate the in vitro assay results with preclinical or clinical outcomes in order to ensure valid predictions about the in vivo potency of therapeutic PnD cells/products in individual disease settings.
Fil: Papait, Andrea. Università Cattolica del Sacro Cuore; Italia
Fil: Silini, Antonietta Rosa. Fondazione Poliambulanza Istituto Ospedaliero; Italia
Fil: Gazouli, Maria. National and Kapodistrian University of Athens; Grecia
Fil: Malvicini, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Università di Padova; Italia
Fil: Muraca, Maurizio. Università di Padova; Italia
Fil: O' Driscoll, Lorraine. Universidad de Dublin; Irlanda
Fil: Pacienza, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Toh, Wei Seong. No especifíca;
Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Ponsaerts, Peter. Universiteit Antwerp; Bélgica
Fil: Parolini, Ornella. Università Cattolica del Sacro Cuore; Italia
Fil: Eissner, Günther. No especifíca;
Fil: Pozzobon, Michela. Università di Padova; Italia
Fil: Lim, Sai Kiang. No especifíca;
Fil: Giebel, Bernd. No especifíca;
Materia
EXOSOMES
EXTRACELLULAR VESICLES
FUNCTIONAL ASSAYS
IMMUNOMODULATION
MECHANISMS OF ACTION
MESENCHYMAL STROMAL CELLS
MICROVESICLES
PERINATAL DERIVATIVES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214461
Acceder
id CONICETDig_22da15ca75cd7ee4878659d327a427aa
oai_identifier_str oai:ri.conicet.gov.ar:11336/214461
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Perinatal derivatives: How to best validate their immunomodulatory functionsPapait, AndreaSilini, Antonietta RosaGazouli, MariaMalvicini, RicardoMuraca, MaurizioO' Driscoll, LorrainePacienza, Natalia AlejandraToh, Wei SeongYannarelli, Gustavo GabrielPonsaerts, PeterParolini, OrnellaEissner, GüntherPozzobon, MichelaLim, Sai KiangGiebel, BerndEXOSOMESEXTRACELLULAR VESICLESFUNCTIONAL ASSAYSIMMUNOMODULATIONMECHANISMS OF ACTIONMESENCHYMAL STROMAL CELLSMICROVESICLESPERINATAL DERIVATIVEShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Perinatal tissues, mainly the placenta and umbilical cord, contain a variety of different somatic stem and progenitor cell types, including those of the hematopoietic system, multipotent mesenchymal stromal cells (MSCs), epithelial cells and amnion epithelial cells. Several of these perinatal derivatives (PnDs), as well as their secreted products, have been reported to exert immunomodulatory therapeutic and regenerative functions in a variety of pre-clinical disease models. Following experience with MSCs and their extracellular vesicle (EV) products, successful clinical translation of PnDs will require robust functional assays that are predictive for the relevant therapeutic potency. Using the examples of T cell and monocyte/macrophage assays, we here discuss several assay relevant parameters for assessing the immunomodulatory activities of PnDs. Furthermore, we highlight the need to correlate the in vitro assay results with preclinical or clinical outcomes in order to ensure valid predictions about the in vivo potency of therapeutic PnD cells/products in individual disease settings.Fil: Papait, Andrea. Università Cattolica del Sacro Cuore; ItaliaFil: Silini, Antonietta Rosa. Fondazione Poliambulanza Istituto Ospedaliero; ItaliaFil: Gazouli, Maria. National and Kapodistrian University of Athens; GreciaFil: Malvicini, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Università di Padova; ItaliaFil: Muraca, Maurizio. Università di Padova; ItaliaFil: O' Driscoll, Lorraine. Universidad de Dublin; IrlandaFil: Pacienza, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Toh, Wei Seong. No especifíca;Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Ponsaerts, Peter. Universiteit Antwerp; BélgicaFil: Parolini, Ornella. Università Cattolica del Sacro Cuore; ItaliaFil: Eissner, Günther. No especifíca;Fil: Pozzobon, Michela. Università di Padova; ItaliaFil: Lim, Sai Kiang. No especifíca;Fil: Giebel, Bernd. No especifíca;Frontiers Media2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214461Papait, Andrea; Silini, Antonietta Rosa; Gazouli, Maria; Malvicini, Ricardo; Muraca, Maurizio; et al.; Perinatal derivatives: How to best validate their immunomodulatory functions; Frontiers Media; Frontiers in Bioengineering and Biotechnology; 10; 9-2022; 1-152296-4185CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fbioe.2022.981061info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:34Zoai:ri.conicet.gov.ar:11336/214461instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:34.721CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Perinatal derivatives: How to best validate their immunomodulatory functions
title Perinatal derivatives: How to best validate their immunomodulatory functions
spellingShingle Perinatal derivatives: How to best validate their immunomodulatory functions
Papait, Andrea
EXOSOMES
EXTRACELLULAR VESICLES
FUNCTIONAL ASSAYS
IMMUNOMODULATION
MECHANISMS OF ACTION
MESENCHYMAL STROMAL CELLS
MICROVESICLES
PERINATAL DERIVATIVES
title_short Perinatal derivatives: How to best validate their immunomodulatory functions
title_full Perinatal derivatives: How to best validate their immunomodulatory functions
title_fullStr Perinatal derivatives: How to best validate their immunomodulatory functions
title_full_unstemmed Perinatal derivatives: How to best validate their immunomodulatory functions
title_sort Perinatal derivatives: How to best validate their immunomodulatory functions
dc.creator.none.fl_str_mv Papait, Andrea
Silini, Antonietta Rosa
Gazouli, Maria
Malvicini, Ricardo
Muraca, Maurizio
O' Driscoll, Lorraine
Pacienza, Natalia Alejandra
Toh, Wei Seong
Yannarelli, Gustavo Gabriel
Ponsaerts, Peter
Parolini, Ornella
Eissner, Günther
Pozzobon, Michela
Lim, Sai Kiang
Giebel, Bernd
author Papait, Andrea
author_facet Papait, Andrea
Silini, Antonietta Rosa
Gazouli, Maria
Malvicini, Ricardo
Muraca, Maurizio
O' Driscoll, Lorraine
Pacienza, Natalia Alejandra
Toh, Wei Seong
Yannarelli, Gustavo Gabriel
Ponsaerts, Peter
Parolini, Ornella
Eissner, Günther
Pozzobon, Michela
Lim, Sai Kiang
Giebel, Bernd
author_role author
author2 Silini, Antonietta Rosa
Gazouli, Maria
Malvicini, Ricardo
Muraca, Maurizio
O' Driscoll, Lorraine
Pacienza, Natalia Alejandra
Toh, Wei Seong
Yannarelli, Gustavo Gabriel
Ponsaerts, Peter
Parolini, Ornella
Eissner, Günther
Pozzobon, Michela
Lim, Sai Kiang
Giebel, Bernd
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EXOSOMES
EXTRACELLULAR VESICLES
FUNCTIONAL ASSAYS
IMMUNOMODULATION
MECHANISMS OF ACTION
MESENCHYMAL STROMAL CELLS
MICROVESICLES
PERINATAL DERIVATIVES
topic EXOSOMES
EXTRACELLULAR VESICLES
FUNCTIONAL ASSAYS
IMMUNOMODULATION
MECHANISMS OF ACTION
MESENCHYMAL STROMAL CELLS
MICROVESICLES
PERINATAL DERIVATIVES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Perinatal tissues, mainly the placenta and umbilical cord, contain a variety of different somatic stem and progenitor cell types, including those of the hematopoietic system, multipotent mesenchymal stromal cells (MSCs), epithelial cells and amnion epithelial cells. Several of these perinatal derivatives (PnDs), as well as their secreted products, have been reported to exert immunomodulatory therapeutic and regenerative functions in a variety of pre-clinical disease models. Following experience with MSCs and their extracellular vesicle (EV) products, successful clinical translation of PnDs will require robust functional assays that are predictive for the relevant therapeutic potency. Using the examples of T cell and monocyte/macrophage assays, we here discuss several assay relevant parameters for assessing the immunomodulatory activities of PnDs. Furthermore, we highlight the need to correlate the in vitro assay results with preclinical or clinical outcomes in order to ensure valid predictions about the in vivo potency of therapeutic PnD cells/products in individual disease settings.
Fil: Papait, Andrea. Università Cattolica del Sacro Cuore; Italia
Fil: Silini, Antonietta Rosa. Fondazione Poliambulanza Istituto Ospedaliero; Italia
Fil: Gazouli, Maria. National and Kapodistrian University of Athens; Grecia
Fil: Malvicini, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Università di Padova; Italia
Fil: Muraca, Maurizio. Università di Padova; Italia
Fil: O' Driscoll, Lorraine. Universidad de Dublin; Irlanda
Fil: Pacienza, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Toh, Wei Seong. No especifíca;
Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Ponsaerts, Peter. Universiteit Antwerp; Bélgica
Fil: Parolini, Ornella. Università Cattolica del Sacro Cuore; Italia
Fil: Eissner, Günther. No especifíca;
Fil: Pozzobon, Michela. Università di Padova; Italia
Fil: Lim, Sai Kiang. No especifíca;
Fil: Giebel, Bernd. No especifíca;
description Perinatal tissues, mainly the placenta and umbilical cord, contain a variety of different somatic stem and progenitor cell types, including those of the hematopoietic system, multipotent mesenchymal stromal cells (MSCs), epithelial cells and amnion epithelial cells. Several of these perinatal derivatives (PnDs), as well as their secreted products, have been reported to exert immunomodulatory therapeutic and regenerative functions in a variety of pre-clinical disease models. Following experience with MSCs and their extracellular vesicle (EV) products, successful clinical translation of PnDs will require robust functional assays that are predictive for the relevant therapeutic potency. Using the examples of T cell and monocyte/macrophage assays, we here discuss several assay relevant parameters for assessing the immunomodulatory activities of PnDs. Furthermore, we highlight the need to correlate the in vitro assay results with preclinical or clinical outcomes in order to ensure valid predictions about the in vivo potency of therapeutic PnD cells/products in individual disease settings.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214461
Papait, Andrea; Silini, Antonietta Rosa; Gazouli, Maria; Malvicini, Ricardo; Muraca, Maurizio; et al.; Perinatal derivatives: How to best validate their immunomodulatory functions; Frontiers Media; Frontiers in Bioengineering and Biotechnology; 10; 9-2022; 1-15
2296-4185
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214461
identifier_str_mv Papait, Andrea; Silini, Antonietta Rosa; Gazouli, Maria; Malvicini, Ricardo; Muraca, Maurizio; et al.; Perinatal derivatives: How to best validate their immunomodulatory functions; Frontiers Media; Frontiers in Bioengineering and Biotechnology; 10; 9-2022; 1-15
2296-4185
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fbioe.2022.981061
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269103130148864
score 13.13397

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