Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)

Autores
Escalante, Jenny; Hamza, Mase; Nishimura, Brent; Melecio, Meghan; Davies Sala, Carol Giselle; Tuttobene, Marisel Romina; Subils, Tomás; Traglia, German Matias; Pham, Chloe; Sieira, Rodrigo; Actis, Luis A.; Bonomo, Robert A.; Tolmasky, Marcelo E.; Ramirez, María Soledad
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major human pathogen and a researchpriority for developing new antimicrobial agents. CRAB is a causative agent of a variety of infections in different body sites. One of the manifestations is catheter-associated urinary tract infection, which exposes the bacteria to the host’s urine, creating a particular environment. Exposure of two CRAB clinical isolates, AB5075 and AMA40, to human urine (HU) resulted in the differential expression levels of 264 and 455 genes, respectively, of which 112 were common to both strains. Genes within this group play roles in metabolic pathways such as phenylacetic acid (PAA) catabolism, the Hut system, the tricarboxylic acid (TCA) cycle, and other processes like quorum sensing and biofilm formation. These results indicate that the presence of HU induces numerous adaptive changes in gene expression of the infecting bacteria. These changes presumably help bacteria establish and thrive in the hostile conditions in the urinary tract. These analyses advance our understanding of CRAB’s metabolic adaptations to human fluids, as well as expand knowledge on bacterial responses to distinct human fluids containing different concentrations of human serum albumin (HSA).
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Hamza, Mase. California State University; Estados Unidos
Fil: Nishimura, Brent. California State University; Estados Unidos
Fil: Melecio, Meghan. California State University; Estados Unidos
Fil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina. Universidad de la República; Uruguay
Fil: Pham, Chloe. California State University; Estados Unidos
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Actis, Luis A.. University of Miami; Estados Unidos
Fil: Bonomo, Robert A.. Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados Unidos
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Ramirez, María Soledad. California State University; Estados Unidos
Materia
ACINETOBACTER BAUMANNII
HUMAN URINE
METABOLIC ADAPTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266467
Acceder
id CONICETDig_22a7206d8935297d07d3f4daa7ae733e
oai_identifier_str oai:ri.conicet.gov.ar:11336/266467
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)Escalante, JennyHamza, MaseNishimura, BrentMelecio, MeghanDavies Sala, Carol GiselleTuttobene, Marisel RominaSubils, TomásTraglia, German MatiasPham, ChloeSieira, RodrigoActis, Luis A.Bonomo, Robert A.Tolmasky, Marcelo E.Ramirez, María SoledadACINETOBACTER BAUMANNIIHUMAN URINEMETABOLIC ADAPTATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major human pathogen and a researchpriority for developing new antimicrobial agents. CRAB is a causative agent of a variety of infections in different body sites. One of the manifestations is catheter-associated urinary tract infection, which exposes the bacteria to the host’s urine, creating a particular environment. Exposure of two CRAB clinical isolates, AB5075 and AMA40, to human urine (HU) resulted in the differential expression levels of 264 and 455 genes, respectively, of which 112 were common to both strains. Genes within this group play roles in metabolic pathways such as phenylacetic acid (PAA) catabolism, the Hut system, the tricarboxylic acid (TCA) cycle, and other processes like quorum sensing and biofilm formation. These results indicate that the presence of HU induces numerous adaptive changes in gene expression of the infecting bacteria. These changes presumably help bacteria establish and thrive in the hostile conditions in the urinary tract. These analyses advance our understanding of CRAB’s metabolic adaptations to human fluids, as well as expand knowledge on bacterial responses to distinct human fluids containing different concentrations of human serum albumin (HSA).Fil: Escalante, Jenny. California State University; Estados UnidosFil: Hamza, Mase. California State University; Estados UnidosFil: Nishimura, Brent. California State University; Estados UnidosFil: Melecio, Meghan. California State University; Estados UnidosFil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina. Universidad de la República; UruguayFil: Pham, Chloe. California State University; Estados UnidosFil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Actis, Luis A.. University of Miami; Estados UnidosFil: Bonomo, Robert A.. Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados UnidosFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Ramirez, María Soledad. California State University; Estados UnidosNature2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266467Escalante, Jenny; Hamza, Mase; Nishimura, Brent; Melecio, Meghan; Davies Sala, Carol Giselle; et al.; Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU); Nature; Scientific Reports; 14; 1; 8-2024; 1-102045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-70216-winfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-70216-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:46Zoai:ri.conicet.gov.ar:11336/266467instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:47.354CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
title Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
spellingShingle Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
Escalante, Jenny
ACINETOBACTER BAUMANNII
HUMAN URINE
METABOLIC ADAPTATION
title_short Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
title_full Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
title_fullStr Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
title_full_unstemmed Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
title_sort Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
dc.creator.none.fl_str_mv Escalante, Jenny
Hamza, Mase
Nishimura, Brent
Melecio, Meghan
Davies Sala, Carol Giselle
Tuttobene, Marisel Romina
Subils, Tomás
Traglia, German Matias
Pham, Chloe
Sieira, Rodrigo
Actis, Luis A.
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, María Soledad
author Escalante, Jenny
author_facet Escalante, Jenny
Hamza, Mase
Nishimura, Brent
Melecio, Meghan
Davies Sala, Carol Giselle
Tuttobene, Marisel Romina
Subils, Tomás
Traglia, German Matias
Pham, Chloe
Sieira, Rodrigo
Actis, Luis A.
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, María Soledad
author_role author
author2 Hamza, Mase
Nishimura, Brent
Melecio, Meghan
Davies Sala, Carol Giselle
Tuttobene, Marisel Romina
Subils, Tomás
Traglia, German Matias
Pham, Chloe
Sieira, Rodrigo
Actis, Luis A.
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, María Soledad
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
HUMAN URINE
METABOLIC ADAPTATION
topic ACINETOBACTER BAUMANNII
HUMAN URINE
METABOLIC ADAPTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major human pathogen and a researchpriority for developing new antimicrobial agents. CRAB is a causative agent of a variety of infections in different body sites. One of the manifestations is catheter-associated urinary tract infection, which exposes the bacteria to the host’s urine, creating a particular environment. Exposure of two CRAB clinical isolates, AB5075 and AMA40, to human urine (HU) resulted in the differential expression levels of 264 and 455 genes, respectively, of which 112 were common to both strains. Genes within this group play roles in metabolic pathways such as phenylacetic acid (PAA) catabolism, the Hut system, the tricarboxylic acid (TCA) cycle, and other processes like quorum sensing and biofilm formation. These results indicate that the presence of HU induces numerous adaptive changes in gene expression of the infecting bacteria. These changes presumably help bacteria establish and thrive in the hostile conditions in the urinary tract. These analyses advance our understanding of CRAB’s metabolic adaptations to human fluids, as well as expand knowledge on bacterial responses to distinct human fluids containing different concentrations of human serum albumin (HSA).
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Hamza, Mase. California State University; Estados Unidos
Fil: Nishimura, Brent. California State University; Estados Unidos
Fil: Melecio, Meghan. California State University; Estados Unidos
Fil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina. Universidad de la República; Uruguay
Fil: Pham, Chloe. California State University; Estados Unidos
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Actis, Luis A.. University of Miami; Estados Unidos
Fil: Bonomo, Robert A.. Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados Unidos
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Ramirez, María Soledad. California State University; Estados Unidos
description Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major human pathogen and a researchpriority for developing new antimicrobial agents. CRAB is a causative agent of a variety of infections in different body sites. One of the manifestations is catheter-associated urinary tract infection, which exposes the bacteria to the host’s urine, creating a particular environment. Exposure of two CRAB clinical isolates, AB5075 and AMA40, to human urine (HU) resulted in the differential expression levels of 264 and 455 genes, respectively, of which 112 were common to both strains. Genes within this group play roles in metabolic pathways such as phenylacetic acid (PAA) catabolism, the Hut system, the tricarboxylic acid (TCA) cycle, and other processes like quorum sensing and biofilm formation. These results indicate that the presence of HU induces numerous adaptive changes in gene expression of the infecting bacteria. These changes presumably help bacteria establish and thrive in the hostile conditions in the urinary tract. These analyses advance our understanding of CRAB’s metabolic adaptations to human fluids, as well as expand knowledge on bacterial responses to distinct human fluids containing different concentrations of human serum albumin (HSA).
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266467
Escalante, Jenny; Hamza, Mase; Nishimura, Brent; Melecio, Meghan; Davies Sala, Carol Giselle; et al.; Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU); Nature; Scientific Reports; 14; 1; 8-2024; 1-10
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266467
identifier_str_mv Escalante, Jenny; Hamza, Mase; Nishimura, Brent; Melecio, Meghan; Davies Sala, Carol Giselle; et al.; Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU); Nature; Scientific Reports; 14; 1; 8-2024; 1-10
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-70216-w
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-70216-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270170424279040
score 13.13397

Publicaciones similares