NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface

Autores
Blois, Sandra M.; Freitag, Nancy; Tirado-González, Irene; Cheng, Shi-Bin; Heimesaat, Markus M.; Bereswill, Stefan; Rose, Matthias; Conrad, Melanie L.; Barrientos, Gabriela Laura; Sharma, Surendra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
DC-NK cell interactions are thought to influence the development of maternal tolerance and de novo angiogenesis during early gestation. However, it is unclear which mechanism ensures the cooperative dialogue between DC and NK cells at the feto-maternal interface. In this article, we show that uterine NK cells are the key source of IL-10 that is required to regulate DC phenotype and pregnancy success. Upon in vivo expansion of DC during early gestation, NK cells expressed increased levels of IL-10. Exogenous administration of IL-10 was sufficient to overcome early pregnancy failure in dams treated to achieve simultaneous DC expansion and NK cell depletion. Remarkably, DC expansion in IL-10-/- dams provoked pregnancy loss, which could be abrogated by the adoptive transfer of IL-10+/+ NK cells and not by IL-10-/- NK cells. Furthermore, the IL-10 expressing NK cells markedly enhanced angiogenic responses and placental development in DC expanded IL-10-/- dams. Thus, the capacity of NK cells to secrete IL-10 plays a unique role facilitating the DC-NK cell dialogue during the establishment of a healthy gestation.
Fil: Blois, Sandra M.. Charité – Universitätsmedizin Berlin;
Fil: Freitag, Nancy. Charité – Universitätsmedizin Berlin;
Fil: Tirado-González, Irene. Charité – Universitätsmedizin Berlin;
Fil: Cheng, Shi-Bin. University Brown; Estados Unidos
Fil: Heimesaat, Markus M.. Charité – Universitätsmedizin Berlin;
Fil: Bereswill, Stefan. Charité – Universitätsmedizin Berlin;
Fil: Rose, Matthias. Charité – Universitätsmedizin Berlin;
Fil: Conrad, Melanie L.. Charité – Universitätsmedizin Berlin;
Fil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Alemán; Argentina
Fil: Sharma, Surendra. University Brown; Estados Unidos
Materia
INFERTILITY
REPRODUCTIVE DISORDERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76977
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interfaceBlois, Sandra M.Freitag, NancyTirado-González, IreneCheng, Shi-BinHeimesaat, Markus M.Bereswill, StefanRose, MatthiasConrad, Melanie L.Barrientos, Gabriela LauraSharma, SurendraINFERTILITYREPRODUCTIVE DISORDERShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3DC-NK cell interactions are thought to influence the development of maternal tolerance and de novo angiogenesis during early gestation. However, it is unclear which mechanism ensures the cooperative dialogue between DC and NK cells at the feto-maternal interface. In this article, we show that uterine NK cells are the key source of IL-10 that is required to regulate DC phenotype and pregnancy success. Upon in vivo expansion of DC during early gestation, NK cells expressed increased levels of IL-10. Exogenous administration of IL-10 was sufficient to overcome early pregnancy failure in dams treated to achieve simultaneous DC expansion and NK cell depletion. Remarkably, DC expansion in IL-10-/- dams provoked pregnancy loss, which could be abrogated by the adoptive transfer of IL-10+/+ NK cells and not by IL-10-/- NK cells. Furthermore, the IL-10 expressing NK cells markedly enhanced angiogenic responses and placental development in DC expanded IL-10-/- dams. Thus, the capacity of NK cells to secrete IL-10 plays a unique role facilitating the DC-NK cell dialogue during the establishment of a healthy gestation.Fil: Blois, Sandra M.. Charité – Universitätsmedizin Berlin;Fil: Freitag, Nancy. Charité – Universitätsmedizin Berlin;Fil: Tirado-González, Irene. Charité – Universitätsmedizin Berlin;Fil: Cheng, Shi-Bin. University Brown; Estados UnidosFil: Heimesaat, Markus M.. Charité – Universitätsmedizin Berlin;Fil: Bereswill, Stefan. Charité – Universitätsmedizin Berlin;Fil: Rose, Matthias. Charité – Universitätsmedizin Berlin;Fil: Conrad, Melanie L.. Charité – Universitätsmedizin Berlin;Fil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Alemán; ArgentinaFil: Sharma, Surendra. University Brown; Estados UnidosNature Publishing Group2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76977Blois, Sandra M.; Freitag, Nancy; Tirado-González, Irene; Cheng, Shi-Bin; Heimesaat, Markus M.; et al.; NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-92045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-02333-8info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-02333-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:01Zoai:ri.conicet.gov.ar:11336/76977instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:01.634CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
title NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
spellingShingle NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
Blois, Sandra M.
INFERTILITY
REPRODUCTIVE DISORDERS
title_short NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
title_full NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
title_fullStr NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
title_full_unstemmed NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
title_sort NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
dc.creator.none.fl_str_mv Blois, Sandra M.
Freitag, Nancy
Tirado-González, Irene
Cheng, Shi-Bin
Heimesaat, Markus M.
Bereswill, Stefan
Rose, Matthias
Conrad, Melanie L.
Barrientos, Gabriela Laura
Sharma, Surendra
author Blois, Sandra M.
author_facet Blois, Sandra M.
Freitag, Nancy
Tirado-González, Irene
Cheng, Shi-Bin
Heimesaat, Markus M.
Bereswill, Stefan
Rose, Matthias
Conrad, Melanie L.
Barrientos, Gabriela Laura
Sharma, Surendra
author_role author
author2 Freitag, Nancy
Tirado-González, Irene
Cheng, Shi-Bin
Heimesaat, Markus M.
Bereswill, Stefan
Rose, Matthias
Conrad, Melanie L.
Barrientos, Gabriela Laura
Sharma, Surendra
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INFERTILITY
REPRODUCTIVE DISORDERS
topic INFERTILITY
REPRODUCTIVE DISORDERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv DC-NK cell interactions are thought to influence the development of maternal tolerance and de novo angiogenesis during early gestation. However, it is unclear which mechanism ensures the cooperative dialogue between DC and NK cells at the feto-maternal interface. In this article, we show that uterine NK cells are the key source of IL-10 that is required to regulate DC phenotype and pregnancy success. Upon in vivo expansion of DC during early gestation, NK cells expressed increased levels of IL-10. Exogenous administration of IL-10 was sufficient to overcome early pregnancy failure in dams treated to achieve simultaneous DC expansion and NK cell depletion. Remarkably, DC expansion in IL-10-/- dams provoked pregnancy loss, which could be abrogated by the adoptive transfer of IL-10+/+ NK cells and not by IL-10-/- NK cells. Furthermore, the IL-10 expressing NK cells markedly enhanced angiogenic responses and placental development in DC expanded IL-10-/- dams. Thus, the capacity of NK cells to secrete IL-10 plays a unique role facilitating the DC-NK cell dialogue during the establishment of a healthy gestation.
Fil: Blois, Sandra M.. Charité – Universitätsmedizin Berlin;
Fil: Freitag, Nancy. Charité – Universitätsmedizin Berlin;
Fil: Tirado-González, Irene. Charité – Universitätsmedizin Berlin;
Fil: Cheng, Shi-Bin. University Brown; Estados Unidos
Fil: Heimesaat, Markus M.. Charité – Universitätsmedizin Berlin;
Fil: Bereswill, Stefan. Charité – Universitätsmedizin Berlin;
Fil: Rose, Matthias. Charité – Universitätsmedizin Berlin;
Fil: Conrad, Melanie L.. Charité – Universitätsmedizin Berlin;
Fil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Alemán; Argentina
Fil: Sharma, Surendra. University Brown; Estados Unidos
description DC-NK cell interactions are thought to influence the development of maternal tolerance and de novo angiogenesis during early gestation. However, it is unclear which mechanism ensures the cooperative dialogue between DC and NK cells at the feto-maternal interface. In this article, we show that uterine NK cells are the key source of IL-10 that is required to regulate DC phenotype and pregnancy success. Upon in vivo expansion of DC during early gestation, NK cells expressed increased levels of IL-10. Exogenous administration of IL-10 was sufficient to overcome early pregnancy failure in dams treated to achieve simultaneous DC expansion and NK cell depletion. Remarkably, DC expansion in IL-10-/- dams provoked pregnancy loss, which could be abrogated by the adoptive transfer of IL-10+/+ NK cells and not by IL-10-/- NK cells. Furthermore, the IL-10 expressing NK cells markedly enhanced angiogenic responses and placental development in DC expanded IL-10-/- dams. Thus, the capacity of NK cells to secrete IL-10 plays a unique role facilitating the DC-NK cell dialogue during the establishment of a healthy gestation.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76977
Blois, Sandra M.; Freitag, Nancy; Tirado-González, Irene; Cheng, Shi-Bin; Heimesaat, Markus M.; et al.; NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-9
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76977
identifier_str_mv Blois, Sandra M.; Freitag, Nancy; Tirado-González, Irene; Cheng, Shi-Bin; Heimesaat, Markus M.; et al.; NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-9
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-02333-8
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-02333-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270066290196480
score 13.13397

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