A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop

Autores: Palm, Thomas; Hemmer, Kathrin; Winter, Julia; Fricke, Inga B.; Tarbashevich, Katsiaryna; Sadeghi Shakib, Fereshteh; Rudolph, Ina-Maria; Hillje, Anna Lena; de Luca, Paola; Bahnassawy, Lamia’a; Madel, Rabea; Viel, Thomas; de Siervi, Adriana; Jacobs, Andreas H.; Diederichs, Sven; Schwamborn, Jens C.
Año de publicación: 2013
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1–miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation.
Fil: Palm, Thomas. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania
Fil: Hemmer, Kathrin. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania
Fil: Winter, Julia. Helmholtz-University-Group Molecular RNA Biology & Cancer. German Cancer Research Center; Alemania. University of Heidelberg. Institute of Pathology; Alemania
Fil: Fricke, Inga B.. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; Alemania
Fil: Tarbashevich, Katsiaryna. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology; Alemania
Fil: Sadeghi Shakib, Fereshteh. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania
Fil: Rudolph, Ina-Maria. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania
Fil: Hillje, Anna Lena. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania
Fil: de Luca, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bahnassawy, Lamia’a. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania
Fil: Madel, Rabea. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania
Fil: Viel, Thomas. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; Alemania
Fil: de Siervi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Jacobs, Andreas H.. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; Alemania
Fil: Diederichs, Sven. Helmholtz-University-Group Molecular RNA Biology & Cancer. German Cancer Research Center; Alemania. University of Heidelberg. Institute of Pathology; Alemania
Fil: Schwamborn, Jens C.. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania. Interdisciplinary Center for Clinical Research; Alemania
Materia: E2f-1
Neural Stem Cell
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/2750

Acceder

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network_name_str	CONICET Digital (CONICET)
spelling	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loopPalm, ThomasHemmer, KathrinWinter, JuliaFricke, Inga B.Tarbashevich, KatsiarynaSadeghi Shakib, FereshtehRudolph, Ina-MariaHillje, Anna Lenade Luca, PaolaBahnassawy, Lamia’aMadel, RabeaViel, Thomasde Siervi, AdrianaJacobs, Andreas H.Diederichs, SvenSchwamborn, Jens C.E2f-1Neural Stem Cellhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1–miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation.Fil: Palm, Thomas. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; AlemaniaFil: Hemmer, Kathrin. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; AlemaniaFil: Winter, Julia. Helmholtz-University-Group Molecular RNA Biology & Cancer. German Cancer Research Center; Alemania. University of Heidelberg. Institute of Pathology; AlemaniaFil: Fricke, Inga B.. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; AlemaniaFil: Tarbashevich, Katsiaryna. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology; AlemaniaFil: Sadeghi Shakib, Fereshteh. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; AlemaniaFil: Rudolph, Ina-Maria. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; AlemaniaFil: Hillje, Anna Lena. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; AlemaniaFil: de Luca, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bahnassawy, Lamia’a. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; AlemaniaFil: Madel, Rabea. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; AlemaniaFil: Viel, Thomas. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; AlemaniaFil: de Siervi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Jacobs, Andreas H.. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; AlemaniaFil: Diederichs, Sven. Helmholtz-University-Group Molecular RNA Biology & Cancer. German Cancer Research Center; Alemania. University of Heidelberg. Institute of Pathology; AlemaniaFil: Schwamborn, Jens C.. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania. Interdisciplinary Center for Clinical Research; AlemaniaOxford University Press2013-02-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2750Palm, Thomas; Hemmer, Kathrin; Winter, Julia; Fricke, Inga B.; Tarbashevich, Katsiaryna; et al.; A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop; Oxford University Press; Nucleic Acids Research; 41; 6; 8-2-2013; 3699-37120305-10481362-4962enginfo:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/41/6/3699.longinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616707/info:eu-repo/semantics/altIdentifier/doi/10.1093%2Fnar%2Fgkt070info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:50:13Zoai:ri.conicet.gov.ar:11336/2750instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:50:14.135CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
title	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
spellingShingle	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop Palm, Thomas E2f-1 Neural Stem Cell
title_short	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
title_full	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
title_fullStr	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
title_full_unstemmed	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
title_sort	A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
dc.creator.none.fl_str_mv	Palm, Thomas Hemmer, Kathrin Winter, Julia Fricke, Inga B. Tarbashevich, Katsiaryna Sadeghi Shakib, Fereshteh Rudolph, Ina-Maria Hillje, Anna Lena de Luca, Paola Bahnassawy, Lamia’a Madel, Rabea Viel, Thomas de Siervi, Adriana Jacobs, Andreas H. Diederichs, Sven Schwamborn, Jens C.
author	Palm, Thomas
author_facet	Palm, Thomas Hemmer, Kathrin Winter, Julia Fricke, Inga B. Tarbashevich, Katsiaryna Sadeghi Shakib, Fereshteh Rudolph, Ina-Maria Hillje, Anna Lena de Luca, Paola Bahnassawy, Lamia’a Madel, Rabea Viel, Thomas de Siervi, Adriana Jacobs, Andreas H. Diederichs, Sven Schwamborn, Jens C.
author_role	author
author2	Hemmer, Kathrin Winter, Julia Fricke, Inga B. Tarbashevich, Katsiaryna Sadeghi Shakib, Fereshteh Rudolph, Ina-Maria Hillje, Anna Lena de Luca, Paola Bahnassawy, Lamia’a Madel, Rabea Viel, Thomas de Siervi, Adriana Jacobs, Andreas H. Diederichs, Sven Schwamborn, Jens C.
author2_role	author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	E2f-1 Neural Stem Cell
topic	E2f-1 Neural Stem Cell
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1–miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation. Fil: Palm, Thomas. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania Fil: Hemmer, Kathrin. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania Fil: Winter, Julia. Helmholtz-University-Group Molecular RNA Biology & Cancer. German Cancer Research Center; Alemania. University of Heidelberg. Institute of Pathology; Alemania Fil: Fricke, Inga B.. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; Alemania Fil: Tarbashevich, Katsiaryna. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology; Alemania Fil: Sadeghi Shakib, Fereshteh. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania Fil: Rudolph, Ina-Maria. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania Fil: Hillje, Anna Lena. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania Fil: de Luca, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Bahnassawy, Lamia’a. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania Fil: Madel, Rabea. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania Fil: Viel, Thomas. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; Alemania Fil: de Siervi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Jacobs, Andreas H.. Westfälische Wilhelms-Universität Münster. European Institute for Molecular Imaging; Alemania. Interdisciplinary Center for Clinical Research; Alemania Fil: Diederichs, Sven. Helmholtz-University-Group Molecular RNA Biology & Cancer. German Cancer Research Center; Alemania. University of Heidelberg. Institute of Pathology; Alemania Fil: Schwamborn, Jens C.. Westfälische Wilhelms-Universität Münster. Center for Molecular Biology of Inflammation. Institute of Cell Biology. Stem Cell Biology and Regeneration Group; Alemania. Interdisciplinary Center for Clinical Research; Alemania
description	Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1–miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation.
publishDate	2013
dc.date.none.fl_str_mv	2013-02-08
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/2750 Palm, Thomas; Hemmer, Kathrin; Winter, Julia; Fricke, Inga B.; Tarbashevich, Katsiaryna; et al.; A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop; Oxford University Press; Nucleic Acids Research; 41; 6; 8-2-2013; 3699-3712 0305-1048 1362-4962
url	http://hdl.handle.net/11336/2750
identifier_str_mv	Palm, Thomas; Hemmer, Kathrin; Winter, Julia; Fricke, Inga B.; Tarbashevich, Katsiaryna; et al.; A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop; Oxford University Press; Nucleic Acids Research; 41; 6; 8-2-2013; 3699-3712 0305-1048 1362-4962
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/41/6/3699.long info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616707/ info:eu-repo/semantics/altIdentifier/doi/10.1093%2Fnar%2Fgkt070
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Oxford University Press
publisher.none.fl_str_mv	Oxford University Press
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	13.011256

A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop

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