A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria

Autores
Ámon, Judit; Fernández y Martín, Rafael; Bokor, Eszter; Cultrone, Antonietta; Kelly, Joan M.; Flipphi, Michel; Scazzocchio, Claudio; Hamari, Zsuzsanna
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nicotinate degradation has hitherto been elucidated only in bacteria. In the ascomycete Aspergillus nidulans, six loci, hxnS/AN9178 encoding the molybdenum cofactor-containing nicotinate hydroxylase, AN11197 encoding a Cys2/His2 zinc finger regulator HxnR, together with AN11196/hxnZ, AN11188/hxnY, AN11189/hxnP and AN9177/hxnT, are clustered and stringently co-induced by a nicotinate derivative and subject to nitrogen metabolite repression mediated by the GATA factor AreA. These genes are strictly co-regulated by HxnR. Within the hxnR gene, constitutive mutations map in two discrete regions. Aspergillus nidulans is capable of using nicotinate and its oxidation products 6-hydroxynicotinic acid and 2,5-dihydroxypyridine as sole nitrogen sources in an HxnR-dependent way. HxnS is highly similar to HxA, the canonical xanthine dehydrogenase (XDH), and has originated by gene duplication, preceding the origin of the Pezizomycotina. This cluster is conserved with some variations throughout the Aspergillaceae. Our results imply that a fungal pathway has arisen independently from bacterial ones. Significantly, the neo-functionalization of XDH into nicotinate hydroxylase has occurred independently from analogous events in bacteria. This work describes for the first time a gene cluster involved in nicotinate catabolism in a eukaryote and has relevance for the formation and evolution of co-regulated primary metabolic gene clusters and the microbial degradation of N-heterocyclic compounds.
Fil: Ámon, Judit. University of Szeged. Faculty of Science and Informatics; Hungría
Fil: Fernández y Martín, Rafael. Université Paris Sud; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bokor, Eszter. University of Szeged. Faculty of Science and Informatics; Hungría
Fil: Cultrone, Antonietta. Université Paris Sud; Francia
Fil: Kelly, Joan M.. University of Essex; Reino Unido
Fil: Flipphi, Michel. Université Paris Sud; Francia
Fil: Scazzocchio, Claudio. Université Paris Sud; Francia. University of Essex; Reino Unido. Imperial College London; Reino Unido
Fil: Hamari, Zsuzsanna. Université Paris Sud; Francia. University of Szeged. Faculty of Science and Informatics; Hungría
Materia
CONVERGENT EVOLUTION
CYS2HIS2 TRANSCRIPTION FACTOR
NICOTINATE CATABOLIC GENE CLUSTER
NICOTINATE HYDROXYLASE
XANTHINE DEHYDROGENASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59259
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/59259
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteriaÁmon, JuditFernández y Martín, RafaelBokor, EszterCultrone, AntoniettaKelly, Joan M.Flipphi, MichelScazzocchio, ClaudioHamari, ZsuzsannaCONVERGENT EVOLUTIONCYS2HIS2 TRANSCRIPTION FACTORNICOTINATE CATABOLIC GENE CLUSTERNICOTINATE HYDROXYLASEXANTHINE DEHYDROGENASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nicotinate degradation has hitherto been elucidated only in bacteria. In the ascomycete Aspergillus nidulans, six loci, hxnS/AN9178 encoding the molybdenum cofactor-containing nicotinate hydroxylase, AN11197 encoding a Cys2/His2 zinc finger regulator HxnR, together with AN11196/hxnZ, AN11188/hxnY, AN11189/hxnP and AN9177/hxnT, are clustered and stringently co-induced by a nicotinate derivative and subject to nitrogen metabolite repression mediated by the GATA factor AreA. These genes are strictly co-regulated by HxnR. Within the hxnR gene, constitutive mutations map in two discrete regions. Aspergillus nidulans is capable of using nicotinate and its oxidation products 6-hydroxynicotinic acid and 2,5-dihydroxypyridine as sole nitrogen sources in an HxnR-dependent way. HxnS is highly similar to HxA, the canonical xanthine dehydrogenase (XDH), and has originated by gene duplication, preceding the origin of the Pezizomycotina. This cluster is conserved with some variations throughout the Aspergillaceae. Our results imply that a fungal pathway has arisen independently from bacterial ones. Significantly, the neo-functionalization of XDH into nicotinate hydroxylase has occurred independently from analogous events in bacteria. This work describes for the first time a gene cluster involved in nicotinate catabolism in a eukaryote and has relevance for the formation and evolution of co-regulated primary metabolic gene clusters and the microbial degradation of N-heterocyclic compounds.Fil: Ámon, Judit. University of Szeged. Faculty of Science and Informatics; HungríaFil: Fernández y Martín, Rafael. Université Paris Sud; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bokor, Eszter. University of Szeged. Faculty of Science and Informatics; HungríaFil: Cultrone, Antonietta. Université Paris Sud; FranciaFil: Kelly, Joan M.. University of Essex; Reino UnidoFil: Flipphi, Michel. Université Paris Sud; FranciaFil: Scazzocchio, Claudio. Université Paris Sud; Francia. University of Essex; Reino Unido. Imperial College London; Reino UnidoFil: Hamari, Zsuzsanna. Université Paris Sud; Francia. University of Szeged. Faculty of Science and Informatics; HungríaRoyal Society Publishing2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59259Ámon, Judit; Fernández y Martín, Rafael; Bokor, Eszter; Cultrone, Antonietta; Kelly, Joan M.; et al.; A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria; Royal Society Publishing; Open Biology; 7; 12; 12-2017; 1-172046-2441CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1098/rsob.170199info:eu-repo/semantics/altIdentifier/url/http://rsob.royalsocietypublishing.org/content/7/12/170199info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746545/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:04Zoai:ri.conicet.gov.ar:11336/59259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:05.061CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
title A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
spellingShingle A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
Ámon, Judit
CONVERGENT EVOLUTION
CYS2HIS2 TRANSCRIPTION FACTOR
NICOTINATE CATABOLIC GENE CLUSTER
NICOTINATE HYDROXYLASE
XANTHINE DEHYDROGENASE
title_short A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
title_full A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
title_fullStr A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
title_full_unstemmed A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
title_sort A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria
dc.creator.none.fl_str_mv Ámon, Judit
Fernández y Martín, Rafael
Bokor, Eszter
Cultrone, Antonietta
Kelly, Joan M.
Flipphi, Michel
Scazzocchio, Claudio
Hamari, Zsuzsanna
author Ámon, Judit
author_facet Ámon, Judit
Fernández y Martín, Rafael
Bokor, Eszter
Cultrone, Antonietta
Kelly, Joan M.
Flipphi, Michel
Scazzocchio, Claudio
Hamari, Zsuzsanna
author_role author
author2 Fernández y Martín, Rafael
Bokor, Eszter
Cultrone, Antonietta
Kelly, Joan M.
Flipphi, Michel
Scazzocchio, Claudio
Hamari, Zsuzsanna
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CONVERGENT EVOLUTION
CYS2HIS2 TRANSCRIPTION FACTOR
NICOTINATE CATABOLIC GENE CLUSTER
NICOTINATE HYDROXYLASE
XANTHINE DEHYDROGENASE
topic CONVERGENT EVOLUTION
CYS2HIS2 TRANSCRIPTION FACTOR
NICOTINATE CATABOLIC GENE CLUSTER
NICOTINATE HYDROXYLASE
XANTHINE DEHYDROGENASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nicotinate degradation has hitherto been elucidated only in bacteria. In the ascomycete Aspergillus nidulans, six loci, hxnS/AN9178 encoding the molybdenum cofactor-containing nicotinate hydroxylase, AN11197 encoding a Cys2/His2 zinc finger regulator HxnR, together with AN11196/hxnZ, AN11188/hxnY, AN11189/hxnP and AN9177/hxnT, are clustered and stringently co-induced by a nicotinate derivative and subject to nitrogen metabolite repression mediated by the GATA factor AreA. These genes are strictly co-regulated by HxnR. Within the hxnR gene, constitutive mutations map in two discrete regions. Aspergillus nidulans is capable of using nicotinate and its oxidation products 6-hydroxynicotinic acid and 2,5-dihydroxypyridine as sole nitrogen sources in an HxnR-dependent way. HxnS is highly similar to HxA, the canonical xanthine dehydrogenase (XDH), and has originated by gene duplication, preceding the origin of the Pezizomycotina. This cluster is conserved with some variations throughout the Aspergillaceae. Our results imply that a fungal pathway has arisen independently from bacterial ones. Significantly, the neo-functionalization of XDH into nicotinate hydroxylase has occurred independently from analogous events in bacteria. This work describes for the first time a gene cluster involved in nicotinate catabolism in a eukaryote and has relevance for the formation and evolution of co-regulated primary metabolic gene clusters and the microbial degradation of N-heterocyclic compounds.
Fil: Ámon, Judit. University of Szeged. Faculty of Science and Informatics; Hungría
Fil: Fernández y Martín, Rafael. Université Paris Sud; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bokor, Eszter. University of Szeged. Faculty of Science and Informatics; Hungría
Fil: Cultrone, Antonietta. Université Paris Sud; Francia
Fil: Kelly, Joan M.. University of Essex; Reino Unido
Fil: Flipphi, Michel. Université Paris Sud; Francia
Fil: Scazzocchio, Claudio. Université Paris Sud; Francia. University of Essex; Reino Unido. Imperial College London; Reino Unido
Fil: Hamari, Zsuzsanna. Université Paris Sud; Francia. University of Szeged. Faculty of Science and Informatics; Hungría
description Nicotinate degradation has hitherto been elucidated only in bacteria. In the ascomycete Aspergillus nidulans, six loci, hxnS/AN9178 encoding the molybdenum cofactor-containing nicotinate hydroxylase, AN11197 encoding a Cys2/His2 zinc finger regulator HxnR, together with AN11196/hxnZ, AN11188/hxnY, AN11189/hxnP and AN9177/hxnT, are clustered and stringently co-induced by a nicotinate derivative and subject to nitrogen metabolite repression mediated by the GATA factor AreA. These genes are strictly co-regulated by HxnR. Within the hxnR gene, constitutive mutations map in two discrete regions. Aspergillus nidulans is capable of using nicotinate and its oxidation products 6-hydroxynicotinic acid and 2,5-dihydroxypyridine as sole nitrogen sources in an HxnR-dependent way. HxnS is highly similar to HxA, the canonical xanthine dehydrogenase (XDH), and has originated by gene duplication, preceding the origin of the Pezizomycotina. This cluster is conserved with some variations throughout the Aspergillaceae. Our results imply that a fungal pathway has arisen independently from bacterial ones. Significantly, the neo-functionalization of XDH into nicotinate hydroxylase has occurred independently from analogous events in bacteria. This work describes for the first time a gene cluster involved in nicotinate catabolism in a eukaryote and has relevance for the formation and evolution of co-regulated primary metabolic gene clusters and the microbial degradation of N-heterocyclic compounds.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59259
Ámon, Judit; Fernández y Martín, Rafael; Bokor, Eszter; Cultrone, Antonietta; Kelly, Joan M.; et al.; A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria; Royal Society Publishing; Open Biology; 7; 12; 12-2017; 1-17
2046-2441
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59259
identifier_str_mv Ámon, Judit; Fernández y Martín, Rafael; Bokor, Eszter; Cultrone, Antonietta; Kelly, Joan M.; et al.; A eukaryotic nicotinate-inducible gene cluster: Convergent evolution in fungi and bacteria; Royal Society Publishing; Open Biology; 7; 12; 12-2017; 1-17
2046-2441
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1098/rsob.170199
info:eu-repo/semantics/altIdentifier/url/http://rsob.royalsocietypublishing.org/content/7/12/170199
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746545/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society Publishing
publisher.none.fl_str_mv Royal Society Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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