Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction

Autores
Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Calvopina, Manuel; Marco, Jorge Diego; Barroso, Paola Andrea; Gomez, Eduardo; Mimori, Tatsuyuki; Korenaga, Masataka; Iwata, Hiroyuki; Nonaka, Shigeo; Hashiguchi, Yoshihisa
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.
Fil: Kato, Hirotomo. Yamaguchi University; Japón
Fil: Uezato, Hiroshi. University of the Ryukyus; Japón
Fil: Katakura, Ken. Hokkaido University; Japón
Fil: Calvopina, Manuel. Kochi University. Kochi Medical School; Japón
Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Barroso, Paola Andrea. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Gomez, Eduardo. Universidad Católica de Guayaquil; Ecuador
Fil: Mimori, Tatsuyuki. Kumamoto University; Japón
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School; Japón
Fil: Iwata, Hiroyuki. Yamaguchi University; Japón
Fil: Nonaka, Shigeo. University ok the Ryukyus; Japón
Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; Japón
Materia
Tipificación
Leishmania
Flebótomos
PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43443
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43443
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reactionKato, HirotomoUezato, HiroshiKatakura, KenCalvopina, ManuelMarco, Jorge DiegoBarroso, Paola AndreaGomez, EduardoMimori, TatsuyukiKorenaga, MasatakaIwata, HiroyukiNonaka, ShigeoHashiguchi, YoshihisaTipificaciónLeishmaniaFlebótomosPCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.Fil: Kato, Hirotomo. Yamaguchi University; JapónFil: Uezato, Hiroshi. University of the Ryukyus; JapónFil: Katakura, Ken. Hokkaido University; JapónFil: Calvopina, Manuel. Kochi University. Kochi Medical School; JapónFil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Barroso, Paola Andrea. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Gomez, Eduardo. Universidad Católica de Guayaquil; EcuadorFil: Mimori, Tatsuyuki. Kumamoto University; JapónFil: Korenaga, Masataka. Kochi University. Kochi Medical School; JapónFil: Iwata, Hiroyuki. Yamaguchi University; JapónFil: Nonaka, Shigeo. University ok the Ryukyus; JapónFil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; JapónAmerican Society of Tropical Medicine and Hygiene2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43443Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Calvopina, Manuel; Marco, Jorge Diego; et al.; Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 72; 1; 12-2005; 87-930002-9637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2005.72.87info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.2005.72.87info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:37:05Zoai:ri.conicet.gov.ar:11336/43443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:37:06.019CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
title Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
spellingShingle Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
Kato, Hirotomo
Tipificación
Leishmania
Flebótomos
PCR
title_short Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
title_full Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
title_fullStr Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
title_full_unstemmed Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
title_sort Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
dc.creator.none.fl_str_mv Kato, Hirotomo
Uezato, Hiroshi
Katakura, Ken
Calvopina, Manuel
Marco, Jorge Diego
Barroso, Paola Andrea
Gomez, Eduardo
Mimori, Tatsuyuki
Korenaga, Masataka
Iwata, Hiroyuki
Nonaka, Shigeo
Hashiguchi, Yoshihisa
author Kato, Hirotomo
author_facet Kato, Hirotomo
Uezato, Hiroshi
Katakura, Ken
Calvopina, Manuel
Marco, Jorge Diego
Barroso, Paola Andrea
Gomez, Eduardo
Mimori, Tatsuyuki
Korenaga, Masataka
Iwata, Hiroyuki
Nonaka, Shigeo
Hashiguchi, Yoshihisa
author_role author
author2 Uezato, Hiroshi
Katakura, Ken
Calvopina, Manuel
Marco, Jorge Diego
Barroso, Paola Andrea
Gomez, Eduardo
Mimori, Tatsuyuki
Korenaga, Masataka
Iwata, Hiroyuki
Nonaka, Shigeo
Hashiguchi, Yoshihisa
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tipificación
Leishmania
Flebótomos
PCR
topic Tipificación
Leishmania
Flebótomos
PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.
Fil: Kato, Hirotomo. Yamaguchi University; Japón
Fil: Uezato, Hiroshi. University of the Ryukyus; Japón
Fil: Katakura, Ken. Hokkaido University; Japón
Fil: Calvopina, Manuel. Kochi University. Kochi Medical School; Japón
Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Barroso, Paola Andrea. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Gomez, Eduardo. Universidad Católica de Guayaquil; Ecuador
Fil: Mimori, Tatsuyuki. Kumamoto University; Japón
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School; Japón
Fil: Iwata, Hiroyuki. Yamaguchi University; Japón
Fil: Nonaka, Shigeo. University ok the Ryukyus; Japón
Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; Japón
description The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43443
Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Calvopina, Manuel; Marco, Jorge Diego; et al.; Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 72; 1; 12-2005; 87-93
0002-9637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43443
identifier_str_mv Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Calvopina, Manuel; Marco, Jorge Diego; et al.; Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 72; 1; 12-2005; 87-93
0002-9637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2005.72.87
info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.2005.72.87
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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