Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction

Autores
Córdoba Lanús, Elizabeth; Piñero, José Enrique; González, Ana Cristina; Valladares, Basilio; Lizarralde de Grosso, Mercedes; Salomón, Oscar Daniel
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
American cutaneous leishmaniasis (ACL) is an endemic disease in Northern Argentina. We applied the polymerase chain reaction (PCR) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of Tucumán, Argentina. We used primers previously designed to detect a Leishmania-specific 120-base-pair fragment of kinetoplast DNA minicircle, other two primer pairs that amplify kDNA minicircles belonging to the L. braziliensisand L. mexicana complexes respectively, and specific oligonucleotide primers to detect L. (V.) braziliensiswhich amplify the sequence of the ribosomal protein L-14 of this species. The PCR-hybridization showed a sensitivity of 90.5% when compared to the histopathology test which was 61.9%. Five of the total samples analyzed were positive for the L. braziliensis complex whilst none was positive for the L. mexicanacomplex. The specific primers for L. (V.) braziliensisdetected the parasite in four samples. These results are consistent with those reported for close endemic areas and demonstrate that the causative agent of human leishmaniasis in the analyzed cases was L. (V.) braziliensis. PCR should be used as a diagnostic tool for tegumentary leishmaniasis, especially in the mucosal form, and as a valuable technique for the identification of the Leishmania species that causes the disease in certain areas.
Fil: Córdoba Lanús, Elizabeth. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.
Fil: Piñero, José Enrique. Universidad de La Laguna. Departamento de Parasitología; Argentina.
Fil: González, Ana Cristina. Universidad de La Laguna. Departamento de Parasitología; Argentina.
Fil: Valladares, Basilio. Universidad de La Laguna. Departamento de Parasitología; Argentina.
Fil: Lizarralde de Grosso, Mercedes. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.
Fil: Salomón, Oscar Daniel. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación en Endemo-Epidemias; Argentina.
Fuente
Memórias do Instituto Oswaldo Cruz, 2005, 100(2), 187–192.
Materia
Leishmania braziliensis
Leishmaniasis Cutánea
Reacción en Cadena de la Polimerasa
Argentina
Tucumán
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/248
Acceder
id SGCANLIS_0e2e7fdf8452cccd62ee47b3a7602485
oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/248
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reactionCórdoba Lanús, ElizabethPiñero, José EnriqueGonzález, Ana CristinaValladares, BasilioLizarralde de Grosso, MercedesSalomón, Oscar DanielLeishmania braziliensisLeishmaniasis CutáneaReacción en Cadena de la PolimerasaArgentinaTucumánAmerican cutaneous leishmaniasis (ACL) is an endemic disease in Northern Argentina. We applied the polymerase chain reaction (PCR) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of Tucumán, Argentina. We used primers previously designed to detect a Leishmania-specific 120-base-pair fragment of kinetoplast DNA minicircle, other two primer pairs that amplify kDNA minicircles belonging to the L. braziliensisand L. mexicana complexes respectively, and specific oligonucleotide primers to detect L. (V.) braziliensiswhich amplify the sequence of the ribosomal protein L-14 of this species. The PCR-hybridization showed a sensitivity of 90.5% when compared to the histopathology test which was 61.9%. Five of the total samples analyzed were positive for the L. braziliensis complex whilst none was positive for the L. mexicanacomplex. The specific primers for L. (V.) braziliensisdetected the parasite in four samples. These results are consistent with those reported for close endemic areas and demonstrate that the causative agent of human leishmaniasis in the analyzed cases was L. (V.) braziliensis. PCR should be used as a diagnostic tool for tegumentary leishmaniasis, especially in the mucosal form, and as a valuable technique for the identification of the Leishmania species that causes the disease in certain areas.Fil: Córdoba Lanús, Elizabeth. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.Fil: Piñero, José Enrique. Universidad de La Laguna. Departamento de Parasitología; Argentina.Fil: González, Ana Cristina. Universidad de La Laguna. Departamento de Parasitología; Argentina.Fil: Valladares, Basilio. Universidad de La Laguna. Departamento de Parasitología; Argentina.Fil: Lizarralde de Grosso, Mercedes. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.Fil: Salomón, Oscar Daniel. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación en Endemo-Epidemias; Argentina.2005info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0074-0276http://sgc.anlis.gob.ar/handle/123456789/248http://www.scielo.br/pdf/mioc/v100n2/v100n2a13.pdfMemórias do Instituto Oswaldo Cruz, 2005, 100(2), 187–192.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISARGTucumánenginfo:eu-repo/semantics/openAccess2025-09-29T14:29:54Zoai:sgc.anlis.gob.ar:Publications/123456789/248Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:29:55.243Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
title Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
spellingShingle Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
Córdoba Lanús, Elizabeth
Leishmania braziliensis
Leishmaniasis Cutánea
Reacción en Cadena de la Polimerasa
Argentina
Tucumán
title_short Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
title_full Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
title_fullStr Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
title_full_unstemmed Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
title_sort Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
dc.creator.none.fl_str_mv Córdoba Lanús, Elizabeth
Piñero, José Enrique
González, Ana Cristina
Valladares, Basilio
Lizarralde de Grosso, Mercedes
Salomón, Oscar Daniel
author Córdoba Lanús, Elizabeth
author_facet Córdoba Lanús, Elizabeth
Piñero, José Enrique
González, Ana Cristina
Valladares, Basilio
Lizarralde de Grosso, Mercedes
Salomón, Oscar Daniel
author_role author
author2 Piñero, José Enrique
González, Ana Cristina
Valladares, Basilio
Lizarralde de Grosso, Mercedes
Salomón, Oscar Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Leishmania braziliensis
Leishmaniasis Cutánea
Reacción en Cadena de la Polimerasa
Argentina
Tucumán
topic Leishmania braziliensis
Leishmaniasis Cutánea
Reacción en Cadena de la Polimerasa
Argentina
Tucumán
dc.description.none.fl_txt_mv American cutaneous leishmaniasis (ACL) is an endemic disease in Northern Argentina. We applied the polymerase chain reaction (PCR) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of Tucumán, Argentina. We used primers previously designed to detect a Leishmania-specific 120-base-pair fragment of kinetoplast DNA minicircle, other two primer pairs that amplify kDNA minicircles belonging to the L. braziliensisand L. mexicana complexes respectively, and specific oligonucleotide primers to detect L. (V.) braziliensiswhich amplify the sequence of the ribosomal protein L-14 of this species. The PCR-hybridization showed a sensitivity of 90.5% when compared to the histopathology test which was 61.9%. Five of the total samples analyzed were positive for the L. braziliensis complex whilst none was positive for the L. mexicanacomplex. The specific primers for L. (V.) braziliensisdetected the parasite in four samples. These results are consistent with those reported for close endemic areas and demonstrate that the causative agent of human leishmaniasis in the analyzed cases was L. (V.) braziliensis. PCR should be used as a diagnostic tool for tegumentary leishmaniasis, especially in the mucosal form, and as a valuable technique for the identification of the Leishmania species that causes the disease in certain areas.
Fil: Córdoba Lanús, Elizabeth. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.
Fil: Piñero, José Enrique. Universidad de La Laguna. Departamento de Parasitología; Argentina.
Fil: González, Ana Cristina. Universidad de La Laguna. Departamento de Parasitología; Argentina.
Fil: Valladares, Basilio. Universidad de La Laguna. Departamento de Parasitología; Argentina.
Fil: Lizarralde de Grosso, Mercedes. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.
Fil: Salomón, Oscar Daniel. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación en Endemo-Epidemias; Argentina.
description American cutaneous leishmaniasis (ACL) is an endemic disease in Northern Argentina. We applied the polymerase chain reaction (PCR) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of Tucumán, Argentina. We used primers previously designed to detect a Leishmania-specific 120-base-pair fragment of kinetoplast DNA minicircle, other two primer pairs that amplify kDNA minicircles belonging to the L. braziliensisand L. mexicana complexes respectively, and specific oligonucleotide primers to detect L. (V.) braziliensiswhich amplify the sequence of the ribosomal protein L-14 of this species. The PCR-hybridization showed a sensitivity of 90.5% when compared to the histopathology test which was 61.9%. Five of the total samples analyzed were positive for the L. braziliensis complex whilst none was positive for the L. mexicanacomplex. The specific primers for L. (V.) braziliensisdetected the parasite in four samples. These results are consistent with those reported for close endemic areas and demonstrate that the causative agent of human leishmaniasis in the analyzed cases was L. (V.) braziliensis. PCR should be used as a diagnostic tool for tegumentary leishmaniasis, especially in the mucosal form, and as a valuable technique for the identification of the Leishmania species that causes the disease in certain areas.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0074-0276
http://sgc.anlis.gob.ar/handle/123456789/248
http://www.scielo.br/pdf/mioc/v100n2/v100n2a13.pdf
identifier_str_mv 0074-0276
url http://sgc.anlis.gob.ar/handle/123456789/248
http://www.scielo.br/pdf/mioc/v100n2/v100n2a13.pdf
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv ARG
Tucumán
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz, 2005, 100(2), 187–192.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
_version_ 1844621851271102464
score 12.559606

Publicaciones similares