Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile

Autores
Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres, L. Gonzalo; Silva de la Fuente, María Carolina; Lareschi, Marcela; González Acuña, Daniel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rattus rattus is a widely distributed, invasive species that presentsan important role in disease transmission, either directly or through vectorarthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis.Results: Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species (n = 55), and it also presented a high prevalence of Bartonella DNA (P% = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB), followed by cities (14.8% gltA and 11.1% rpoB) and wild areas (7.4% gltA and 14.8% rpoB). The BLAST analysis showed a high similitude (>96%) with four uncharacterized Bartonella genotypes and with two species with zoonotic potential: B. mastomydisand B. tribocorum. The phylogenetic analysis showed a close relationship withB. elizabethae and B. tribocorum. This is the first study to provide evidence of the presence of Bartonella in fleas of R. rattus in Chile, indicating that the villages and arid zone correspond to areas with higher infection risk.
Fil: Moreno Salas, Lucila. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile
Fil: Espinoza Carniglia, Mario Virgilio. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lizama Schmeisser, Nicol. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile
Fil: Torres, L. Gonzalo. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile. Universidad Austral de Chile; Chile
Fil: Silva de la Fuente, María Carolina. Universidad de Concepción; Chile. Universidad San Sebastian.; Chile
Fil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: González Acuña, Daniel. Universidad de Concepción; Chile
Materia
RATTUS
FLEAS
BARTONELLA
PUBLIC HEALTH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121480
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/121480
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In ChileMoreno Salas, LucilaEspinoza Carniglia, Mario VirgilioLizama Schmeisser, NicolTorres, L. GonzaloSilva de la Fuente, María CarolinaLareschi, MarcelaGonzález Acuña, DanielRATTUSFLEASBARTONELLAPUBLIC HEALTHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Rattus rattus is a widely distributed, invasive species that presentsan important role in disease transmission, either directly or through vectorarthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis.Results: Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species (n = 55), and it also presented a high prevalence of Bartonella DNA (P% = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB), followed by cities (14.8% gltA and 11.1% rpoB) and wild areas (7.4% gltA and 14.8% rpoB). The BLAST analysis showed a high similitude (>96%) with four uncharacterized Bartonella genotypes and with two species with zoonotic potential: B. mastomydisand B. tribocorum. The phylogenetic analysis showed a close relationship withB. elizabethae and B. tribocorum. This is the first study to provide evidence of the presence of Bartonella in fleas of R. rattus in Chile, indicating that the villages and arid zone correspond to areas with higher infection risk.Fil: Moreno Salas, Lucila. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; ChileFil: Espinoza Carniglia, Mario Virgilio. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Lizama Schmeisser, Nicol. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; ChileFil: Torres, L. Gonzalo. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile. Universidad Austral de Chile; ChileFil: Silva de la Fuente, María Carolina. Universidad de Concepción; Chile. Universidad San Sebastian.; ChileFil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: González Acuña, Daniel. Universidad de Concepción; ChilePeerJ Inc.2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121480Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres, L. Gonzalo; Silva de la Fuente, María Carolina; et al.; Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile; PeerJ Inc.; PeerJ; 2019; 8; 8-20192167-8359CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/7371info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.7371info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679904/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:56Zoai:ri.conicet.gov.ar:11336/121480instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:56.634CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
title Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
spellingShingle Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
Moreno Salas, Lucila
RATTUS
FLEAS
BARTONELLA
PUBLIC HEALTH
title_short Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
title_full Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
title_fullStr Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
title_full_unstemmed Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
title_sort Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile
dc.creator.none.fl_str_mv Moreno Salas, Lucila
Espinoza Carniglia, Mario Virgilio
Lizama Schmeisser, Nicol
Torres, L. Gonzalo
Silva de la Fuente, María Carolina
Lareschi, Marcela
González Acuña, Daniel
author Moreno Salas, Lucila
author_facet Moreno Salas, Lucila
Espinoza Carniglia, Mario Virgilio
Lizama Schmeisser, Nicol
Torres, L. Gonzalo
Silva de la Fuente, María Carolina
Lareschi, Marcela
González Acuña, Daniel
author_role author
author2 Espinoza Carniglia, Mario Virgilio
Lizama Schmeisser, Nicol
Torres, L. Gonzalo
Silva de la Fuente, María Carolina
Lareschi, Marcela
González Acuña, Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv RATTUS
FLEAS
BARTONELLA
PUBLIC HEALTH
topic RATTUS
FLEAS
BARTONELLA
PUBLIC HEALTH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rattus rattus is a widely distributed, invasive species that presentsan important role in disease transmission, either directly or through vectorarthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis.Results: Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species (n = 55), and it also presented a high prevalence of Bartonella DNA (P% = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB), followed by cities (14.8% gltA and 11.1% rpoB) and wild areas (7.4% gltA and 14.8% rpoB). The BLAST analysis showed a high similitude (>96%) with four uncharacterized Bartonella genotypes and with two species with zoonotic potential: B. mastomydisand B. tribocorum. The phylogenetic analysis showed a close relationship withB. elizabethae and B. tribocorum. This is the first study to provide evidence of the presence of Bartonella in fleas of R. rattus in Chile, indicating that the villages and arid zone correspond to areas with higher infection risk.
Fil: Moreno Salas, Lucila. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile
Fil: Espinoza Carniglia, Mario Virgilio. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lizama Schmeisser, Nicol. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile
Fil: Torres, L. Gonzalo. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile. Universidad Austral de Chile; Chile
Fil: Silva de la Fuente, María Carolina. Universidad de Concepción; Chile. Universidad San Sebastian.; Chile
Fil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: González Acuña, Daniel. Universidad de Concepción; Chile
description Rattus rattus is a widely distributed, invasive species that presentsan important role in disease transmission, either directly or through vectorarthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis.Results: Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species (n = 55), and it also presented a high prevalence of Bartonella DNA (P% = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB), followed by cities (14.8% gltA and 11.1% rpoB) and wild areas (7.4% gltA and 14.8% rpoB). The BLAST analysis showed a high similitude (>96%) with four uncharacterized Bartonella genotypes and with two species with zoonotic potential: B. mastomydisand B. tribocorum. The phylogenetic analysis showed a close relationship withB. elizabethae and B. tribocorum. This is the first study to provide evidence of the presence of Bartonella in fleas of R. rattus in Chile, indicating that the villages and arid zone correspond to areas with higher infection risk.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121480
Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres, L. Gonzalo; Silva de la Fuente, María Carolina; et al.; Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile; PeerJ Inc.; PeerJ; 2019; 8; 8-2019
2167-8359
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121480
identifier_str_mv Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres, L. Gonzalo; Silva de la Fuente, María Carolina; et al.; Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. In Chile; PeerJ Inc.; PeerJ; 2019; 8; 8-2019
2167-8359
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.7371
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679904/
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv PeerJ Inc.
publisher.none.fl_str_mv PeerJ Inc.
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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