Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile

Autores
Millán, Javier Maximiliano; Sepúlveda García, Paulina; Di Cataldo, María Sophia; Canales, Nivia; Sallaberry Pincheira, Nicole; Painean, Javier; Cevidanes, Aitor; Müller, Ananda
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.
Fil: Millán, Javier Maximiliano. Universidad Andrés Bello; Chile. Universidad de Zaragoza; España
Fil: Sepúlveda García, Paulina. Universidad Austral de Chile; Chile
Fil: Di Cataldo, María Sophia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Canales, Nivia. Universidad Austral de Chile; Chile
Fil: Sallaberry Pincheira, Nicole. Universidad Andrés Bello; Chile
Fil: Painean, Javier. Universidad Austral de Chile; Chile
Fil: Cevidanes, Aitor. Centro de Investigación. Neiker - Tecnalia; España
Fil: Müller, Ananda. Universidad Austral de Chile; Chile
Materia
CULPEO
FLEA-BORNE
PSEUDALOPEX
SOUTH AMERICA
VECTOR-BORNE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230725
Acceder
id CONICETDig_1e62b1d6fabe2991c0113b79289ac304
oai_identifier_str oai:ri.conicet.gov.ar:11336/230725
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, ChileMillán, Javier MaximilianoSepúlveda García, PaulinaDi Cataldo, María SophiaCanales, NiviaSallaberry Pincheira, NicolePainean, JavierCevidanes, AitorMüller, AnandaCULPEOFLEA-BORNEPSEUDALOPEXSOUTH AMERICAVECTOR-BORNEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.Fil: Millán, Javier Maximiliano. Universidad Andrés Bello; Chile. Universidad de Zaragoza; EspañaFil: Sepúlveda García, Paulina. Universidad Austral de Chile; ChileFil: Di Cataldo, María Sophia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Canales, Nivia. Universidad Austral de Chile; ChileFil: Sallaberry Pincheira, Nicole. Universidad Andrés Bello; ChileFil: Painean, Javier. Universidad Austral de Chile; ChileFil: Cevidanes, Aitor. Centro de Investigación. Neiker - Tecnalia; EspañaFil: Müller, Ananda. Universidad Austral de Chile; ChileElsevier2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230725Millán, Javier Maximiliano; Sepúlveda García, Paulina; Di Cataldo, María Sophia; Canales, Nivia; Sallaberry Pincheira, Nicole; et al.; Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 96; 5-2023; 1-90147-9571CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2023.101983info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:36Zoai:ri.conicet.gov.ar:11336/230725instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:36.549CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
title Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
spellingShingle Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
Millán, Javier Maximiliano
CULPEO
FLEA-BORNE
PSEUDALOPEX
SOUTH AMERICA
VECTOR-BORNE
title_short Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
title_full Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
title_fullStr Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
title_full_unstemmed Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
title_sort Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
dc.creator.none.fl_str_mv Millán, Javier Maximiliano
Sepúlveda García, Paulina
Di Cataldo, María Sophia
Canales, Nivia
Sallaberry Pincheira, Nicole
Painean, Javier
Cevidanes, Aitor
Müller, Ananda
author Millán, Javier Maximiliano
author_facet Millán, Javier Maximiliano
Sepúlveda García, Paulina
Di Cataldo, María Sophia
Canales, Nivia
Sallaberry Pincheira, Nicole
Painean, Javier
Cevidanes, Aitor
Müller, Ananda
author_role author
author2 Sepúlveda García, Paulina
Di Cataldo, María Sophia
Canales, Nivia
Sallaberry Pincheira, Nicole
Painean, Javier
Cevidanes, Aitor
Müller, Ananda
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CULPEO
FLEA-BORNE
PSEUDALOPEX
SOUTH AMERICA
VECTOR-BORNE
topic CULPEO
FLEA-BORNE
PSEUDALOPEX
SOUTH AMERICA
VECTOR-BORNE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.
Fil: Millán, Javier Maximiliano. Universidad Andrés Bello; Chile. Universidad de Zaragoza; España
Fil: Sepúlveda García, Paulina. Universidad Austral de Chile; Chile
Fil: Di Cataldo, María Sophia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Canales, Nivia. Universidad Austral de Chile; Chile
Fil: Sallaberry Pincheira, Nicole. Universidad Andrés Bello; Chile
Fil: Painean, Javier. Universidad Austral de Chile; Chile
Fil: Cevidanes, Aitor. Centro de Investigación. Neiker - Tecnalia; España
Fil: Müller, Ananda. Universidad Austral de Chile; Chile
description Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230725
Millán, Javier Maximiliano; Sepúlveda García, Paulina; Di Cataldo, María Sophia; Canales, Nivia; Sallaberry Pincheira, Nicole; et al.; Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 96; 5-2023; 1-9
0147-9571
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230725
identifier_str_mv Millán, Javier Maximiliano; Sepúlveda García, Paulina; Di Cataldo, María Sophia; Canales, Nivia; Sallaberry Pincheira, Nicole; et al.; Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 96; 5-2023; 1-9
0147-9571
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2023.101983
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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