Molecular detection of Rickettsia in fleas from micromammals in Chile

Autores
Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres Fuentes, Luis Gonzalo; Silva de La Fuente, María Carolina; Lareschi, Marcela; González Acuña, Daniel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Rickettsial diseases are considered important in public health due to their dispersal capacity determined by the particular characteristics of their reservoirs and/or vectors. Among the latter, fleas play an important role, since the vast majority of species parasitize wild and invasive rodents, so their detection is relevant to be able to monitor potential emerging diseases. The aim of this study was to detect, characterize, and compare Rickettsia spp. from the fleas of micromammals in areas with different human population densities in Chile. Methods: The presence of Rickettsia spp. was evaluated by standard polymerase chain reaction (PCR) and sequencing in 1315 fleas collected from 1512 micromammals in 29 locations, with different human population densities in Chile. A generalized linear model (GLM) was used to identify the variables that may explain Rickettsia prevalence in fleas. Results: DNA of Rickettsia spp. was identified in 13.2% (174 of 1315) of fleas tested. Fifteen flea species were found to be Rickettsia-positive. The prevalence of Rickettsia spp. was higher in winter, semi-arid region and natural areas, and the infection levels in fleas varied between species of flea. The prevalence of Rickettsia among flea species ranged between 0–35.1%. Areas of lower human density showed the highest prevalence of Rickettsia. The phylogenetic tree showed two well-differentiated clades with Rickettsia bellii positioned as basal in one clade. The second clade was subdivided into two subclades of species related to Rickettsia of the spotted fever group. Conclusions: To our knowledge, this is the first report of the occurrence and molecular characterization of Rickettsia spp. in 15 flea species of micromammals in Chile. In this study, fleas were detected carrying Rickettsia DNA with zoonotic potential, mainly in villages and natural areas of Chile. Considering that there are differences in the prevalence of Rickettsia in fleas associated with different factors, more investigations are needed to further understand the ecology of Rickettsia in fleas and their implications for human health.[Figure not available: see fulltext.]
Fil: Moreno Salas, Lucila. Universidad de Concepción; Chile
Fil: Espinoza Carniglia, Mario Virgilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lizama Schmeisser, Nicol. Universidad de Concepción; Chile
Fil: Torres Fuentes, Luis Gonzalo. Universidad Austral de Chile; Chile
Fil: Silva de La Fuente, María Carolina. Universidad Austral de Chile; Chile
Fil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: González Acuña, Daniel. Universidad de Concepción; Chile
Materia
BACTERIA
FLEAS
MARSUPIALS
PATHOGEN
RODENTS
VECTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143552
Acceder
id CONICETDig_11fe65fcfec5b510b309c8fe5589e39f
oai_identifier_str oai:ri.conicet.gov.ar:11336/143552
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular detection of Rickettsia in fleas from micromammals in ChileMoreno Salas, LucilaEspinoza Carniglia, Mario VirgilioLizama Schmeisser, NicolTorres Fuentes, Luis GonzaloSilva de La Fuente, María CarolinaLareschi, MarcelaGonzález Acuña, DanielBACTERIAFLEASMARSUPIALSPATHOGENRODENTSVECTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Rickettsial diseases are considered important in public health due to their dispersal capacity determined by the particular characteristics of their reservoirs and/or vectors. Among the latter, fleas play an important role, since the vast majority of species parasitize wild and invasive rodents, so their detection is relevant to be able to monitor potential emerging diseases. The aim of this study was to detect, characterize, and compare Rickettsia spp. from the fleas of micromammals in areas with different human population densities in Chile. Methods: The presence of Rickettsia spp. was evaluated by standard polymerase chain reaction (PCR) and sequencing in 1315 fleas collected from 1512 micromammals in 29 locations, with different human population densities in Chile. A generalized linear model (GLM) was used to identify the variables that may explain Rickettsia prevalence in fleas. Results: DNA of Rickettsia spp. was identified in 13.2% (174 of 1315) of fleas tested. Fifteen flea species were found to be Rickettsia-positive. The prevalence of Rickettsia spp. was higher in winter, semi-arid region and natural areas, and the infection levels in fleas varied between species of flea. The prevalence of Rickettsia among flea species ranged between 0–35.1%. Areas of lower human density showed the highest prevalence of Rickettsia. The phylogenetic tree showed two well-differentiated clades with Rickettsia bellii positioned as basal in one clade. The second clade was subdivided into two subclades of species related to Rickettsia of the spotted fever group. Conclusions: To our knowledge, this is the first report of the occurrence and molecular characterization of Rickettsia spp. in 15 flea species of micromammals in Chile. In this study, fleas were detected carrying Rickettsia DNA with zoonotic potential, mainly in villages and natural areas of Chile. Considering that there are differences in the prevalence of Rickettsia in fleas associated with different factors, more investigations are needed to further understand the ecology of Rickettsia in fleas and their implications for human health.[Figure not available: see fulltext.]Fil: Moreno Salas, Lucila. Universidad de Concepción; ChileFil: Espinoza Carniglia, Mario Virgilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Lizama Schmeisser, Nicol. Universidad de Concepción; ChileFil: Torres Fuentes, Luis Gonzalo. Universidad Austral de Chile; ChileFil: Silva de La Fuente, María Carolina. Universidad Austral de Chile; ChileFil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: González Acuña, Daniel. Universidad de Concepción; ChileBioMed Central2020-10-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143552Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres Fuentes, Luis Gonzalo; Silva de La Fuente, María Carolina; et al.; Molecular detection of Rickettsia in fleas from micromammals in Chile; BioMed Central; Parasites and Vectors; 13; 1; 17-10-2020; 1-211756-3305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04388-5info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-020-04388-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:24Zoai:ri.conicet.gov.ar:11336/143552instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:25.114CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular detection of Rickettsia in fleas from micromammals in Chile
title Molecular detection of Rickettsia in fleas from micromammals in Chile
spellingShingle Molecular detection of Rickettsia in fleas from micromammals in Chile
Moreno Salas, Lucila
BACTERIA
FLEAS
MARSUPIALS
PATHOGEN
RODENTS
VECTORS
title_short Molecular detection of Rickettsia in fleas from micromammals in Chile
title_full Molecular detection of Rickettsia in fleas from micromammals in Chile
title_fullStr Molecular detection of Rickettsia in fleas from micromammals in Chile
title_full_unstemmed Molecular detection of Rickettsia in fleas from micromammals in Chile
title_sort Molecular detection of Rickettsia in fleas from micromammals in Chile
dc.creator.none.fl_str_mv Moreno Salas, Lucila
Espinoza Carniglia, Mario Virgilio
Lizama Schmeisser, Nicol
Torres Fuentes, Luis Gonzalo
Silva de La Fuente, María Carolina
Lareschi, Marcela
González Acuña, Daniel
author Moreno Salas, Lucila
author_facet Moreno Salas, Lucila
Espinoza Carniglia, Mario Virgilio
Lizama Schmeisser, Nicol
Torres Fuentes, Luis Gonzalo
Silva de La Fuente, María Carolina
Lareschi, Marcela
González Acuña, Daniel
author_role author
author2 Espinoza Carniglia, Mario Virgilio
Lizama Schmeisser, Nicol
Torres Fuentes, Luis Gonzalo
Silva de La Fuente, María Carolina
Lareschi, Marcela
González Acuña, Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIA
FLEAS
MARSUPIALS
PATHOGEN
RODENTS
VECTORS
topic BACTERIA
FLEAS
MARSUPIALS
PATHOGEN
RODENTS
VECTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Rickettsial diseases are considered important in public health due to their dispersal capacity determined by the particular characteristics of their reservoirs and/or vectors. Among the latter, fleas play an important role, since the vast majority of species parasitize wild and invasive rodents, so their detection is relevant to be able to monitor potential emerging diseases. The aim of this study was to detect, characterize, and compare Rickettsia spp. from the fleas of micromammals in areas with different human population densities in Chile. Methods: The presence of Rickettsia spp. was evaluated by standard polymerase chain reaction (PCR) and sequencing in 1315 fleas collected from 1512 micromammals in 29 locations, with different human population densities in Chile. A generalized linear model (GLM) was used to identify the variables that may explain Rickettsia prevalence in fleas. Results: DNA of Rickettsia spp. was identified in 13.2% (174 of 1315) of fleas tested. Fifteen flea species were found to be Rickettsia-positive. The prevalence of Rickettsia spp. was higher in winter, semi-arid region and natural areas, and the infection levels in fleas varied between species of flea. The prevalence of Rickettsia among flea species ranged between 0–35.1%. Areas of lower human density showed the highest prevalence of Rickettsia. The phylogenetic tree showed two well-differentiated clades with Rickettsia bellii positioned as basal in one clade. The second clade was subdivided into two subclades of species related to Rickettsia of the spotted fever group. Conclusions: To our knowledge, this is the first report of the occurrence and molecular characterization of Rickettsia spp. in 15 flea species of micromammals in Chile. In this study, fleas were detected carrying Rickettsia DNA with zoonotic potential, mainly in villages and natural areas of Chile. Considering that there are differences in the prevalence of Rickettsia in fleas associated with different factors, more investigations are needed to further understand the ecology of Rickettsia in fleas and their implications for human health.[Figure not available: see fulltext.]
Fil: Moreno Salas, Lucila. Universidad de Concepción; Chile
Fil: Espinoza Carniglia, Mario Virgilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lizama Schmeisser, Nicol. Universidad de Concepción; Chile
Fil: Torres Fuentes, Luis Gonzalo. Universidad Austral de Chile; Chile
Fil: Silva de La Fuente, María Carolina. Universidad Austral de Chile; Chile
Fil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: González Acuña, Daniel. Universidad de Concepción; Chile
description Background: Rickettsial diseases are considered important in public health due to their dispersal capacity determined by the particular characteristics of their reservoirs and/or vectors. Among the latter, fleas play an important role, since the vast majority of species parasitize wild and invasive rodents, so their detection is relevant to be able to monitor potential emerging diseases. The aim of this study was to detect, characterize, and compare Rickettsia spp. from the fleas of micromammals in areas with different human population densities in Chile. Methods: The presence of Rickettsia spp. was evaluated by standard polymerase chain reaction (PCR) and sequencing in 1315 fleas collected from 1512 micromammals in 29 locations, with different human population densities in Chile. A generalized linear model (GLM) was used to identify the variables that may explain Rickettsia prevalence in fleas. Results: DNA of Rickettsia spp. was identified in 13.2% (174 of 1315) of fleas tested. Fifteen flea species were found to be Rickettsia-positive. The prevalence of Rickettsia spp. was higher in winter, semi-arid region and natural areas, and the infection levels in fleas varied between species of flea. The prevalence of Rickettsia among flea species ranged between 0–35.1%. Areas of lower human density showed the highest prevalence of Rickettsia. The phylogenetic tree showed two well-differentiated clades with Rickettsia bellii positioned as basal in one clade. The second clade was subdivided into two subclades of species related to Rickettsia of the spotted fever group. Conclusions: To our knowledge, this is the first report of the occurrence and molecular characterization of Rickettsia spp. in 15 flea species of micromammals in Chile. In this study, fleas were detected carrying Rickettsia DNA with zoonotic potential, mainly in villages and natural areas of Chile. Considering that there are differences in the prevalence of Rickettsia in fleas associated with different factors, more investigations are needed to further understand the ecology of Rickettsia in fleas and their implications for human health.[Figure not available: see fulltext.]
publishDate 2020
dc.date.none.fl_str_mv 2020-10-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143552
Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres Fuentes, Luis Gonzalo; Silva de La Fuente, María Carolina; et al.; Molecular detection of Rickettsia in fleas from micromammals in Chile; BioMed Central; Parasites and Vectors; 13; 1; 17-10-2020; 1-21
1756-3305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143552
identifier_str_mv Moreno Salas, Lucila; Espinoza Carniglia, Mario Virgilio; Lizama Schmeisser, Nicol; Torres Fuentes, Luis Gonzalo; Silva de La Fuente, María Carolina; et al.; Molecular detection of Rickettsia in fleas from micromammals in Chile; BioMed Central; Parasites and Vectors; 13; 1; 17-10-2020; 1-21
1756-3305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04388-5
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-020-04388-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981114375831552
score 12.48226

Publicaciones similares