In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species

Autores
Clavin, María L.; Redko, Flavia; Acevedo, Cristina; Martino, Virginia Susana; Gorzalczany, Susana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eupatorium subhastatum, E.laevigatum, E. macrocephalum, E. hecatanthum and E. candolleanum are used in traditional medicine for the treatment of ailments related to inflammation. Their aqueous and dichloromethane extracts were evaluated for in vivo anti-inflammatory activity in the carrageenan-induced paw oedema and in the TPA-induced ear oedema tests. Dichloromethane extracts of the five species significantly inhibited the mouse ear oedema, being E. subhastatum, E. laevigatum, and E. hecatanthum the most active ones, with maximum inhibitions of 86.4, 89.7, 94.4% and ED50 of 0.35, 0.51 and 0.92 mg/ear, respectively. The dichloromethane and aqueous extracts of E. subhastatum were the only active ones in the carrageenan-induced paw edema assay, both producing a moderate oedema inhibition of about 37%. No acute toxicity was observed for any of the aqueous extracts at a dose of 2g/kg. DAD-HPLC analysis of the five active dichloromethane extracts led to the identification of the anti-inflammatory compounds jaceosidin, nepetin, hispidulin and/or eupatilin in these species. The presence of these active compounds in the DCM extracts could justify at least in part their anti-inflammatory activity and the medicinal uses of these species for the treatment of local inflammations.
Fil: Clavin, María L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina
Fil: Redko, Flavia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina
Fil: Acevedo, Cristina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Martino, Virginia Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina
Fil: Gorzalczany, Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Eupatorium
Flavonoids
Edema
Toxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12546
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12546
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium SpeciesClavin, María L.Redko, FlaviaAcevedo, CristinaMartino, Virginia SusanaGorzalczany, SusanaEupatoriumFlavonoidsEdemaToxicityhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Eupatorium subhastatum, E.laevigatum, E. macrocephalum, E. hecatanthum and E. candolleanum are used in traditional medicine for the treatment of ailments related to inflammation. Their aqueous and dichloromethane extracts were evaluated for in vivo anti-inflammatory activity in the carrageenan-induced paw oedema and in the TPA-induced ear oedema tests. Dichloromethane extracts of the five species significantly inhibited the mouse ear oedema, being E. subhastatum, E. laevigatum, and E. hecatanthum the most active ones, with maximum inhibitions of 86.4, 89.7, 94.4% and ED50 of 0.35, 0.51 and 0.92 mg/ear, respectively. The dichloromethane and aqueous extracts of E. subhastatum were the only active ones in the carrageenan-induced paw edema assay, both producing a moderate oedema inhibition of about 37%. No acute toxicity was observed for any of the aqueous extracts at a dose of 2g/kg. DAD-HPLC analysis of the five active dichloromethane extracts led to the identification of the anti-inflammatory compounds jaceosidin, nepetin, hispidulin and/or eupatilin in these species. The presence of these active compounds in the DCM extracts could justify at least in part their anti-inflammatory activity and the medicinal uses of these species for the treatment of local inflammations.Fil: Clavin, María L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Redko, Flavia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Acevedo, Cristina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Martino, Virginia Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Gorzalczany, Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaElsevier2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12546Clavin, María L.; Redko, Flavia; Acevedo, Cristina; Martino, Virginia Susana; Gorzalczany, Susana; In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species; Elsevier; Pharmacognosy journal; 5; 2; 3-2013; 91-930975-3575enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0975357513000217info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phcgj.2013.03.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:47Zoai:ri.conicet.gov.ar:11336/12546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:47.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
title In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
spellingShingle In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
Clavin, María L.
Eupatorium
Flavonoids
Edema
Toxicity
title_short In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
title_full In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
title_fullStr In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
title_full_unstemmed In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
title_sort In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species
dc.creator.none.fl_str_mv Clavin, María L.
Redko, Flavia
Acevedo, Cristina
Martino, Virginia Susana
Gorzalczany, Susana
author Clavin, María L.
author_facet Clavin, María L.
Redko, Flavia
Acevedo, Cristina
Martino, Virginia Susana
Gorzalczany, Susana
author_role author
author2 Redko, Flavia
Acevedo, Cristina
Martino, Virginia Susana
Gorzalczany, Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Eupatorium
Flavonoids
Edema
Toxicity
topic Eupatorium
Flavonoids
Edema
Toxicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Eupatorium subhastatum, E.laevigatum, E. macrocephalum, E. hecatanthum and E. candolleanum are used in traditional medicine for the treatment of ailments related to inflammation. Their aqueous and dichloromethane extracts were evaluated for in vivo anti-inflammatory activity in the carrageenan-induced paw oedema and in the TPA-induced ear oedema tests. Dichloromethane extracts of the five species significantly inhibited the mouse ear oedema, being E. subhastatum, E. laevigatum, and E. hecatanthum the most active ones, with maximum inhibitions of 86.4, 89.7, 94.4% and ED50 of 0.35, 0.51 and 0.92 mg/ear, respectively. The dichloromethane and aqueous extracts of E. subhastatum were the only active ones in the carrageenan-induced paw edema assay, both producing a moderate oedema inhibition of about 37%. No acute toxicity was observed for any of the aqueous extracts at a dose of 2g/kg. DAD-HPLC analysis of the five active dichloromethane extracts led to the identification of the anti-inflammatory compounds jaceosidin, nepetin, hispidulin and/or eupatilin in these species. The presence of these active compounds in the DCM extracts could justify at least in part their anti-inflammatory activity and the medicinal uses of these species for the treatment of local inflammations.
Fil: Clavin, María L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina
Fil: Redko, Flavia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina
Fil: Acevedo, Cristina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Martino, Virginia Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina
Fil: Gorzalczany, Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Eupatorium subhastatum, E.laevigatum, E. macrocephalum, E. hecatanthum and E. candolleanum are used in traditional medicine for the treatment of ailments related to inflammation. Their aqueous and dichloromethane extracts were evaluated for in vivo anti-inflammatory activity in the carrageenan-induced paw oedema and in the TPA-induced ear oedema tests. Dichloromethane extracts of the five species significantly inhibited the mouse ear oedema, being E. subhastatum, E. laevigatum, and E. hecatanthum the most active ones, with maximum inhibitions of 86.4, 89.7, 94.4% and ED50 of 0.35, 0.51 and 0.92 mg/ear, respectively. The dichloromethane and aqueous extracts of E. subhastatum were the only active ones in the carrageenan-induced paw edema assay, both producing a moderate oedema inhibition of about 37%. No acute toxicity was observed for any of the aqueous extracts at a dose of 2g/kg. DAD-HPLC analysis of the five active dichloromethane extracts led to the identification of the anti-inflammatory compounds jaceosidin, nepetin, hispidulin and/or eupatilin in these species. The presence of these active compounds in the DCM extracts could justify at least in part their anti-inflammatory activity and the medicinal uses of these species for the treatment of local inflammations.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12546
Clavin, María L.; Redko, Flavia; Acevedo, Cristina; Martino, Virginia Susana; Gorzalczany, Susana; In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species; Elsevier; Pharmacognosy journal; 5; 2; 3-2013; 91-93
0975-3575
url http://hdl.handle.net/11336/12546
identifier_str_mv Clavin, María L.; Redko, Flavia; Acevedo, Cristina; Martino, Virginia Susana; Gorzalczany, Susana; In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species; Elsevier; Pharmacognosy journal; 5; 2; 3-2013; 91-93
0975-3575
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0975357513000217
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phcgj.2013.03.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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