Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease

Autores
Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways.
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Cremaschi, Fabián. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Area de Neurología Clínica y Quirúrgica; Argentina;
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Cabrera, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Materia
Progesterone
Dopamine
Glutamate
Gaba
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2322

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's diseaseCasas, Sebastián MarceloGiuliani, Fernando AlfredoCremaschi, FabiánYunes, Roberto Miguel FedericoCabrera, RicardoProgesteroneDopamineGlutamateGabahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways.Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Fil: Cremaschi, Fabián. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Area de Neurología Clínica y Quirúrgica; Argentina;Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Fil: Cabrera, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Maney Publishing2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2322Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo; Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease; Maney Publishing; Neurological Research.; 35; 7; 9-2013; 719-7250161-6412enginfo:eu-repo/semantics/altIdentifier/doi/10.1179/1743132812Y.0000000142info:eu-repo/semantics/altIdentifier/url/http://www.maneyonline.com/doi/abs/10.1179/1743132812Y.0000000142?journalCode=nerinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:24Zoai:ri.conicet.gov.ar:11336/2322instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:24.322CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
title Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
spellingShingle Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
Casas, Sebastián Marcelo
Progesterone
Dopamine
Glutamate
Gaba
title_short Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
title_full Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
title_fullStr Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
title_full_unstemmed Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
title_sort Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
dc.creator.none.fl_str_mv Casas, Sebastián Marcelo
Giuliani, Fernando Alfredo
Cremaschi, Fabián
Yunes, Roberto Miguel Federico
Cabrera, Ricardo
author Casas, Sebastián Marcelo
author_facet Casas, Sebastián Marcelo
Giuliani, Fernando Alfredo
Cremaschi, Fabián
Yunes, Roberto Miguel Federico
Cabrera, Ricardo
author_role author
author2 Giuliani, Fernando Alfredo
Cremaschi, Fabián
Yunes, Roberto Miguel Federico
Cabrera, Ricardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Progesterone
Dopamine
Glutamate
Gaba
topic Progesterone
Dopamine
Glutamate
Gaba
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways.
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Cremaschi, Fabián. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Area de Neurología Clínica y Quirúrgica; Argentina;
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Cabrera, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
description Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2322
Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo; Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease; Maney Publishing; Neurological Research.; 35; 7; 9-2013; 719-725
0161-6412
url http://hdl.handle.net/11336/2322
identifier_str_mv Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo; Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease; Maney Publishing; Neurological Research.; 35; 7; 9-2013; 719-725
0161-6412
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1179/1743132812Y.0000000142
info:eu-repo/semantics/altIdentifier/url/http://www.maneyonline.com/doi/abs/10.1179/1743132812Y.0000000142?journalCode=ner
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Maney Publishing
publisher.none.fl_str_mv Maney Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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