Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease
- Autores
- Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways.
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Cremaschi, Fabián. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Area de Neurología Clínica y Quirúrgica; Argentina;
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;
Fil: Cabrera, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina; - Materia
-
Progesterone
Dopamine
Glutamate
Gaba - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2322
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Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's diseaseCasas, Sebastián MarceloGiuliani, Fernando AlfredoCremaschi, FabiánYunes, Roberto Miguel FedericoCabrera, RicardoProgesteroneDopamineGlutamateGabahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways.Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Fil: Cremaschi, Fabián. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Area de Neurología Clínica y Quirúrgica; Argentina;Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Fil: Cabrera, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina;Maney Publishing2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2322Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo; Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease; Maney Publishing; Neurological Research.; 35; 7; 9-2013; 719-7250161-6412enginfo:eu-repo/semantics/altIdentifier/doi/10.1179/1743132812Y.0000000142info:eu-repo/semantics/altIdentifier/url/http://www.maneyonline.com/doi/abs/10.1179/1743132812Y.0000000142?journalCode=nerinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:24Zoai:ri.conicet.gov.ar:11336/2322instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:24.322CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease |
title |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease |
spellingShingle |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease Casas, Sebastián Marcelo Progesterone Dopamine Glutamate Gaba |
title_short |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease |
title_full |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease |
title_fullStr |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease |
title_full_unstemmed |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease |
title_sort |
Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease |
dc.creator.none.fl_str_mv |
Casas, Sebastián Marcelo Giuliani, Fernando Alfredo Cremaschi, Fabián Yunes, Roberto Miguel Federico Cabrera, Ricardo |
author |
Casas, Sebastián Marcelo |
author_facet |
Casas, Sebastián Marcelo Giuliani, Fernando Alfredo Cremaschi, Fabián Yunes, Roberto Miguel Federico Cabrera, Ricardo |
author_role |
author |
author2 |
Giuliani, Fernando Alfredo Cremaschi, Fabián Yunes, Roberto Miguel Federico Cabrera, Ricardo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Progesterone Dopamine Glutamate Gaba |
topic |
Progesterone Dopamine Glutamate Gaba |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways. Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina; Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina; Fil: Cremaschi, Fabián. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Area de Neurología Clínica y Quirúrgica; Argentina; Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina; Fil: Cabrera, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad de Mendoza. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina; |
description |
Objectives: Progesterone has been reported to have a neuroprotective role in depression-like rats in a hemiparkinsonian model of the disease. In this work, we investigate if this hormone affects the three principal neurochemicals striatal systems (dopaminergic, glutamatergic, and GABAergic) that are involved in the physiopathology of the disease in a hemiparkinsonim male rat model at 8 weeks post-chemical injury. Methods: For this purpose, we design three experimental groups: (1) sham group; (2) hemiparkinsonian group; and (3) hemiparkinsonian group subcutaneously injected with progesterone at 7 days post- chemical injury. Animals were tested in an automated rotational device at 8 weeks post-chemical injury. After behavioral test, Kz-evoked [3H]-dopamine, [3H]-glutamate, and [3H]-gamma aminobutyric acid release from striatum slices were analyzed by superfusion experiments. Results: The hemiparkinsonian group showed distinctive alterations that are produced by neurodegenera- tion of left nigrostriatal dopaminergic pathway by 6-hydroxydopamine hydrobromide (6-OHDA). On the other hand, the administration of progesterone 7 days after the injection of the neurotoxin was able to (1) improve the Kz-evoked [3H]-dopamine release from the damaged striata (left); (2) avoid significant increase in the Kz-evoked [3H]-glutamate release from the left striata; and (3) progesterone does not modify the Kz-evoked [3H]-gamma aminobutyric acid release from the left striata. Discussion: These results suggest that progesterone does have neuroprotective and neuromodulatory effects on striatal neurotransmission systems in the hemiparkinsonian male rats. The possible mechanisms would involve genomic and non-genomic actions of this neuroactive steroid which would modulate the activity of dopaminergic, glutamatergic, and GABAergic pathways. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2322 Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo; Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease; Maney Publishing; Neurological Research.; 35; 7; 9-2013; 719-725 0161-6412 |
url |
http://hdl.handle.net/11336/2322 |
identifier_str_mv |
Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Cremaschi, Fabián; Yunes, Roberto Miguel Federico; Cabrera, Ricardo; Neuromodulatory effect of progesterone on the dopaminergic, glutamatergic, and GABAergic activities in a male rat model of Parkinson's disease; Maney Publishing; Neurological Research.; 35; 7; 9-2013; 719-725 0161-6412 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1179/1743132812Y.0000000142 info:eu-repo/semantics/altIdentifier/url/http://www.maneyonline.com/doi/abs/10.1179/1743132812Y.0000000142?journalCode=ner |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Maney Publishing |
publisher.none.fl_str_mv |
Maney Publishing |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268729038077952 |
score |
13.13397 |