Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate

Autores
Garzón, Antonela Guadalupe; Veras, Flávio F.; Brandelli, Adriano; Drago, Silvina Rosa
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The identification of antioxidant, antidiabetogenic and antimicrobial peptides obtained from sorghum spent grain (SSG) protein hydrolysate was performed. Antioxidant activity (AOA) was evaluated by ABTS+ radical cation scavenging, antidiabetogenic activity (ADA) by dipeptidyl peptidase IV (DPP-IV) inhibition, and antimicrobial activity (AMA) through the minimum inhibitory concentration (MIC) method against 2 Gram-negative and 3 Gram-positive bacteria. SSG peptides were fractionated using a Sephadex G-25 molecular exclusion column, and fractions collected (F1–F6) were evaluated through AOA, ADA and AMA assays. The chromatographic process allowed increasing 20% the AOA and 35% the ADA in F2, and 90% the AMA in fractions F5 and F6. The most active fractions (F2, F5 and F6) were evaluated using LC-ESI-Q-TOF tandem mass spectrometry, which allowed identifying six new peptides with high bioactivity. Moreover, the docking refinement protocol showed that identified peptides could interact with the DPP-IV enzyme, two of them in a competitive inhibition mode. Sorghum spent grain (SSG) could be an important source of bioactive peptides. Additionally, this is the first report indicating the presence of antibacterial peptides encrypted in sorghum proteins.
Fil: Garzón, Antonela Guadalupe. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Veras, Flávio F.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Drago, Silvina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Materia
BIOACTIVE PEPTIDES
DOCKING
FRACTIONATION
SORGHUM
TANDEM MASS SPECTROMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215661
Acceder
id CONICETDig_1de6f1eda710d04acffbbdc8e43e012d
oai_identifier_str oai:ri.conicet.gov.ar:11336/215661
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysateGarzón, Antonela GuadalupeVeras, Flávio F.Brandelli, AdrianoDrago, Silvina RosaBIOACTIVE PEPTIDESDOCKINGFRACTIONATIONSORGHUMTANDEM MASS SPECTROMETRYhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The identification of antioxidant, antidiabetogenic and antimicrobial peptides obtained from sorghum spent grain (SSG) protein hydrolysate was performed. Antioxidant activity (AOA) was evaluated by ABTS+ radical cation scavenging, antidiabetogenic activity (ADA) by dipeptidyl peptidase IV (DPP-IV) inhibition, and antimicrobial activity (AMA) through the minimum inhibitory concentration (MIC) method against 2 Gram-negative and 3 Gram-positive bacteria. SSG peptides were fractionated using a Sephadex G-25 molecular exclusion column, and fractions collected (F1–F6) were evaluated through AOA, ADA and AMA assays. The chromatographic process allowed increasing 20% the AOA and 35% the ADA in F2, and 90% the AMA in fractions F5 and F6. The most active fractions (F2, F5 and F6) were evaluated using LC-ESI-Q-TOF tandem mass spectrometry, which allowed identifying six new peptides with high bioactivity. Moreover, the docking refinement protocol showed that identified peptides could interact with the DPP-IV enzyme, two of them in a competitive inhibition mode. Sorghum spent grain (SSG) could be an important source of bioactive peptides. Additionally, this is the first report indicating the presence of antibacterial peptides encrypted in sorghum proteins.Fil: Garzón, Antonela Guadalupe. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Veras, Flávio F.. Universidade Federal do Rio Grande do Sul; BrasilFil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; BrasilFil: Drago, Silvina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaElsevier Science2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215661Garzón, Antonela Guadalupe; Veras, Flávio F.; Brandelli, Adriano; Drago, Silvina Rosa; Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate; Elsevier Science; LWT - Food Science and Technology; 153; 1-2022; 1-90023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S002364382101567Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2021.112414info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:16Zoai:ri.conicet.gov.ar:11336/215661instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:16.553CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
title Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
spellingShingle Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
Garzón, Antonela Guadalupe
BIOACTIVE PEPTIDES
DOCKING
FRACTIONATION
SORGHUM
TANDEM MASS SPECTROMETRY
title_short Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
title_full Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
title_fullStr Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
title_full_unstemmed Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
title_sort Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
dc.creator.none.fl_str_mv Garzón, Antonela Guadalupe
Veras, Flávio F.
Brandelli, Adriano
Drago, Silvina Rosa
author Garzón, Antonela Guadalupe
author_facet Garzón, Antonela Guadalupe
Veras, Flávio F.
Brandelli, Adriano
Drago, Silvina Rosa
author_role author
author2 Veras, Flávio F.
Brandelli, Adriano
Drago, Silvina Rosa
author2_role author
author
author
dc.subject.none.fl_str_mv BIOACTIVE PEPTIDES
DOCKING
FRACTIONATION
SORGHUM
TANDEM MASS SPECTROMETRY
topic BIOACTIVE PEPTIDES
DOCKING
FRACTIONATION
SORGHUM
TANDEM MASS SPECTROMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The identification of antioxidant, antidiabetogenic and antimicrobial peptides obtained from sorghum spent grain (SSG) protein hydrolysate was performed. Antioxidant activity (AOA) was evaluated by ABTS+ radical cation scavenging, antidiabetogenic activity (ADA) by dipeptidyl peptidase IV (DPP-IV) inhibition, and antimicrobial activity (AMA) through the minimum inhibitory concentration (MIC) method against 2 Gram-negative and 3 Gram-positive bacteria. SSG peptides were fractionated using a Sephadex G-25 molecular exclusion column, and fractions collected (F1–F6) were evaluated through AOA, ADA and AMA assays. The chromatographic process allowed increasing 20% the AOA and 35% the ADA in F2, and 90% the AMA in fractions F5 and F6. The most active fractions (F2, F5 and F6) were evaluated using LC-ESI-Q-TOF tandem mass spectrometry, which allowed identifying six new peptides with high bioactivity. Moreover, the docking refinement protocol showed that identified peptides could interact with the DPP-IV enzyme, two of them in a competitive inhibition mode. Sorghum spent grain (SSG) could be an important source of bioactive peptides. Additionally, this is the first report indicating the presence of antibacterial peptides encrypted in sorghum proteins.
Fil: Garzón, Antonela Guadalupe. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Veras, Flávio F.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Drago, Silvina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
description The identification of antioxidant, antidiabetogenic and antimicrobial peptides obtained from sorghum spent grain (SSG) protein hydrolysate was performed. Antioxidant activity (AOA) was evaluated by ABTS+ radical cation scavenging, antidiabetogenic activity (ADA) by dipeptidyl peptidase IV (DPP-IV) inhibition, and antimicrobial activity (AMA) through the minimum inhibitory concentration (MIC) method against 2 Gram-negative and 3 Gram-positive bacteria. SSG peptides were fractionated using a Sephadex G-25 molecular exclusion column, and fractions collected (F1–F6) were evaluated through AOA, ADA and AMA assays. The chromatographic process allowed increasing 20% the AOA and 35% the ADA in F2, and 90% the AMA in fractions F5 and F6. The most active fractions (F2, F5 and F6) were evaluated using LC-ESI-Q-TOF tandem mass spectrometry, which allowed identifying six new peptides with high bioactivity. Moreover, the docking refinement protocol showed that identified peptides could interact with the DPP-IV enzyme, two of them in a competitive inhibition mode. Sorghum spent grain (SSG) could be an important source of bioactive peptides. Additionally, this is the first report indicating the presence of antibacterial peptides encrypted in sorghum proteins.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215661
Garzón, Antonela Guadalupe; Veras, Flávio F.; Brandelli, Adriano; Drago, Silvina Rosa; Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate; Elsevier Science; LWT - Food Science and Technology; 153; 1-2022; 1-9
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215661
identifier_str_mv Garzón, Antonela Guadalupe; Veras, Flávio F.; Brandelli, Adriano; Drago, Silvina Rosa; Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate; Elsevier Science; LWT - Food Science and Technology; 153; 1-2022; 1-9
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S002364382101567X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2021.112414
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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