Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes

Autores
Falcone Ferreyra, María Lorena; Casas, María Isabel; Qüesta, Julia Irene; Herrera, Andrea Lorena; DeBlasio, Stacy; Wang, Jing; Jackson, David; Grotewold, Erich; Casati, Paula
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flavonoids are specialized compounds widely distributed and with diverse functions throughout the plant kingdom and with several benefits for human health. In particular, flavonols, synthesized by flavonol synthase (FLS), protect plants against UV-B radiation and are essential for male fertility in maize and other plants. We have recently characterized a UV-B inducible ZmFLS1, corresponding to the first to be described in monocot plants. Interestingly, the new assembly of the B73 maize genome revealed the presence of a second putative FLS gene (ZmFLS2), with very high identity with ZmFLS1. ZmFLSs expression was analyzed in different maize tissues, and by combining EMSA assays and transient expression experiments, we show that both genes are direct targets of anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. ZmFLS expression analyses show higher levels of both transcripts in high altitude landraces than inbred lines, and both genes are regulated by UV-B radiation in all lines analyzed. Moreover, the high sequence conservation of the ZmFLS promoters between maize lines suggests that the differences observed in ZmFLS expression are due to allelic variations in the transcription factors that regulate their activities. Finally, we generated pFLS1::FLS1-RFP transgenic plants and analyzed ZmFLS1 expression in different maize tissues; we found that this enzyme is localized in the ER and the perinuclear region.
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casas, María Isabel. Ohio State University; Estados Unidos
Fil: Qüesta, Julia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Herrera, Andrea Lorena. Ohio State University; Estados Unidos
Fil: DeBlasio, Stacy. Cold Spring Harbor; Estados Unidos
Fil: Wang, Jing. Cold Spring Harbor; Estados Unidos
Fil: Jackson, David. Cold Spring Harbor; Estados Unidos
Fil: Grotewold, Erich. Ohio State University; Estados Unidos
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Materia
Favonoids
Flavonols
Flavonols synthase
UV-B
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273219
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/273219
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase GenesFalcone Ferreyra, María LorenaCasas, María IsabelQüesta, Julia IreneHerrera, Andrea LorenaDeBlasio, StacyWang, JingJackson, DavidGrotewold, ErichCasati, PaulaFavonoidsFlavonolsFlavonols synthaseUV-Bhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flavonoids are specialized compounds widely distributed and with diverse functions throughout the plant kingdom and with several benefits for human health. In particular, flavonols, synthesized by flavonol synthase (FLS), protect plants against UV-B radiation and are essential for male fertility in maize and other plants. We have recently characterized a UV-B inducible ZmFLS1, corresponding to the first to be described in monocot plants. Interestingly, the new assembly of the B73 maize genome revealed the presence of a second putative FLS gene (ZmFLS2), with very high identity with ZmFLS1. ZmFLSs expression was analyzed in different maize tissues, and by combining EMSA assays and transient expression experiments, we show that both genes are direct targets of anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. ZmFLS expression analyses show higher levels of both transcripts in high altitude landraces than inbred lines, and both genes are regulated by UV-B radiation in all lines analyzed. Moreover, the high sequence conservation of the ZmFLS promoters between maize lines suggests that the differences observed in ZmFLS expression are due to allelic variations in the transcription factors that regulate their activities. Finally, we generated pFLS1::FLS1-RFP transgenic plants and analyzed ZmFLS1 expression in different maize tissues; we found that this enzyme is localized in the ER and the perinuclear region.Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Casas, María Isabel. Ohio State University; Estados UnidosFil: Qüesta, Julia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Herrera, Andrea Lorena. Ohio State University; Estados UnidosFil: DeBlasio, Stacy. Cold Spring Harbor; Estados UnidosFil: Wang, Jing. Cold Spring Harbor; Estados UnidosFil: Jackson, David. Cold Spring Harbor; Estados UnidosFil: Grotewold, Erich. Ohio State University; Estados UnidosFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFrontiers Media2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273219Falcone Ferreyra, María Lorena; Casas, María Isabel; Qüesta, Julia Irene; Herrera, Andrea Lorena; DeBlasio, Stacy; et al.; Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes; Frontiers Media; Frontiers in Plant Science; 3; 6-2012; 1-261664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2012.00101/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2012.00101info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:34:51Zoai:ri.conicet.gov.ar:11336/273219instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:34:51.813CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
title Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
spellingShingle Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
Falcone Ferreyra, María Lorena
Favonoids
Flavonols
Flavonols synthase
UV-B
title_short Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
title_full Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
title_fullStr Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
title_full_unstemmed Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
title_sort Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes
dc.creator.none.fl_str_mv Falcone Ferreyra, María Lorena
Casas, María Isabel
Qüesta, Julia Irene
Herrera, Andrea Lorena
DeBlasio, Stacy
Wang, Jing
Jackson, David
Grotewold, Erich
Casati, Paula
author Falcone Ferreyra, María Lorena
author_facet Falcone Ferreyra, María Lorena
Casas, María Isabel
Qüesta, Julia Irene
Herrera, Andrea Lorena
DeBlasio, Stacy
Wang, Jing
Jackson, David
Grotewold, Erich
Casati, Paula
author_role author
author2 Casas, María Isabel
Qüesta, Julia Irene
Herrera, Andrea Lorena
DeBlasio, Stacy
Wang, Jing
Jackson, David
Grotewold, Erich
Casati, Paula
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Favonoids
Flavonols
Flavonols synthase
UV-B
topic Favonoids
Flavonols
Flavonols synthase
UV-B
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flavonoids are specialized compounds widely distributed and with diverse functions throughout the plant kingdom and with several benefits for human health. In particular, flavonols, synthesized by flavonol synthase (FLS), protect plants against UV-B radiation and are essential for male fertility in maize and other plants. We have recently characterized a UV-B inducible ZmFLS1, corresponding to the first to be described in monocot plants. Interestingly, the new assembly of the B73 maize genome revealed the presence of a second putative FLS gene (ZmFLS2), with very high identity with ZmFLS1. ZmFLSs expression was analyzed in different maize tissues, and by combining EMSA assays and transient expression experiments, we show that both genes are direct targets of anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. ZmFLS expression analyses show higher levels of both transcripts in high altitude landraces than inbred lines, and both genes are regulated by UV-B radiation in all lines analyzed. Moreover, the high sequence conservation of the ZmFLS promoters between maize lines suggests that the differences observed in ZmFLS expression are due to allelic variations in the transcription factors that regulate their activities. Finally, we generated pFLS1::FLS1-RFP transgenic plants and analyzed ZmFLS1 expression in different maize tissues; we found that this enzyme is localized in the ER and the perinuclear region.
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casas, María Isabel. Ohio State University; Estados Unidos
Fil: Qüesta, Julia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Herrera, Andrea Lorena. Ohio State University; Estados Unidos
Fil: DeBlasio, Stacy. Cold Spring Harbor; Estados Unidos
Fil: Wang, Jing. Cold Spring Harbor; Estados Unidos
Fil: Jackson, David. Cold Spring Harbor; Estados Unidos
Fil: Grotewold, Erich. Ohio State University; Estados Unidos
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
description Flavonoids are specialized compounds widely distributed and with diverse functions throughout the plant kingdom and with several benefits for human health. In particular, flavonols, synthesized by flavonol synthase (FLS), protect plants against UV-B radiation and are essential for male fertility in maize and other plants. We have recently characterized a UV-B inducible ZmFLS1, corresponding to the first to be described in monocot plants. Interestingly, the new assembly of the B73 maize genome revealed the presence of a second putative FLS gene (ZmFLS2), with very high identity with ZmFLS1. ZmFLSs expression was analyzed in different maize tissues, and by combining EMSA assays and transient expression experiments, we show that both genes are direct targets of anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. ZmFLS expression analyses show higher levels of both transcripts in high altitude landraces than inbred lines, and both genes are regulated by UV-B radiation in all lines analyzed. Moreover, the high sequence conservation of the ZmFLS promoters between maize lines suggests that the differences observed in ZmFLS expression are due to allelic variations in the transcription factors that regulate their activities. Finally, we generated pFLS1::FLS1-RFP transgenic plants and analyzed ZmFLS1 expression in different maize tissues; we found that this enzyme is localized in the ER and the perinuclear region.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273219
Falcone Ferreyra, María Lorena; Casas, María Isabel; Qüesta, Julia Irene; Herrera, Andrea Lorena; DeBlasio, Stacy; et al.; Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes; Frontiers Media; Frontiers in Plant Science; 3; 6-2012; 1-26
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273219
identifier_str_mv Falcone Ferreyra, María Lorena; Casas, María Isabel; Qüesta, Julia Irene; Herrera, Andrea Lorena; DeBlasio, Stacy; et al.; Evolution and Expression of Tandem Duplicated Maize Flavonol Synthase Genes; Frontiers Media; Frontiers in Plant Science; 3; 6-2012; 1-26
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2012.00101/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2012.00101
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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