Cloning and characterization of a UV-B-inducible maize flavonol synthase

Autores
Falcone Ferreyra, María Lorena; Rius, Sebastian Pablo; Emiliani, Julia; Pourcel, Lucille; Feller, Antje; Morohashi, Kengo; Casati, Paula; Grotewold, Erich
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flavonols are important compounds for male fertility in maize and other crops, and they also contribute to protecting plants from UV-B radiation. However, little continues to be known on how maize and other grasses synthesize flavonols, and how flavonol biosynthesis is regulated. By homology to an Arabidopsis flavonol synthase (FLS1), we cloned a maize gene encoding a product (ZmFLS1) capable of converting the dihydrokaempferol (DHK) and dihydroquercetin (DHQ) dihydroflavonols to the corresponding flavonols, kaempferol (K) and quercetin (Q). Moreover, ZmFLS1 partially complements the flavonol deficiency of Arabidopsis fls1 mutant and restores anthocyanin accumulation to normal levels. We demonstrate that ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. Indeed, using chromatin immunoprecipitation (ChIP) experiments, we demonstrate that ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes, revealing a similar control as for earlier steps in the maize flavonoid pathway. Highlighting the importance of flavonols in UV-B protection, we also show that ZmFLS1 is induced in maize seedlings by UV-B, and that this induction is in part mediated by the increased expression of the P1, B and PL1 regulators. Together, our results identify a key flavonoid biosynthetic enzyme so far missed in maize and other monocots, and illustrate mechanisms by which flavonol accumulation is controlled in maize.
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Rius, Sebastian Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Emiliani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Pourcel, Lucille. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
Fil: Feller, Antje. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
Fil: Morohashi, Kengo. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Grotewold, Erich. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
Materia
FLAVONOL
SYNTHASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276651
Acceder
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spelling Cloning and characterization of a UV-B-inducible maize flavonol synthaseFalcone Ferreyra, María LorenaRius, Sebastian PabloEmiliani, JuliaPourcel, LucilleFeller, AntjeMorohashi, KengoCasati, PaulaGrotewold, ErichFLAVONOLSYNTHASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flavonols are important compounds for male fertility in maize and other crops, and they also contribute to protecting plants from UV-B radiation. However, little continues to be known on how maize and other grasses synthesize flavonols, and how flavonol biosynthesis is regulated. By homology to an Arabidopsis flavonol synthase (FLS1), we cloned a maize gene encoding a product (ZmFLS1) capable of converting the dihydrokaempferol (DHK) and dihydroquercetin (DHQ) dihydroflavonols to the corresponding flavonols, kaempferol (K) and quercetin (Q). Moreover, ZmFLS1 partially complements the flavonol deficiency of Arabidopsis fls1 mutant and restores anthocyanin accumulation to normal levels. We demonstrate that ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. Indeed, using chromatin immunoprecipitation (ChIP) experiments, we demonstrate that ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes, revealing a similar control as for earlier steps in the maize flavonoid pathway. Highlighting the importance of flavonols in UV-B protection, we also show that ZmFLS1 is induced in maize seedlings by UV-B, and that this induction is in part mediated by the increased expression of the P1, B and PL1 regulators. Together, our results identify a key flavonoid biosynthetic enzyme so far missed in maize and other monocots, and illustrate mechanisms by which flavonol accumulation is controlled in maize.Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Rius, Sebastian Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Emiliani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Pourcel, Lucille. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados UnidosFil: Feller, Antje. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados UnidosFil: Morohashi, Kengo. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados UnidosFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Grotewold, Erich. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados UnidosWiley Blackwell Publishing, Inc2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276651Falcone Ferreyra, María Lorena; Rius, Sebastian Pablo; Emiliani, Julia; Pourcel, Lucille; Feller, Antje; et al.; Cloning and characterization of a UV-B-inducible maize flavonol synthase; Wiley Blackwell Publishing, Inc; Plant Journal; 62; 1; 4-2010; 77-910960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2010.04133.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2010.04133.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:19:50Zoai:ri.conicet.gov.ar:11336/276651instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:19:51.254CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cloning and characterization of a UV-B-inducible maize flavonol synthase
title Cloning and characterization of a UV-B-inducible maize flavonol synthase
spellingShingle Cloning and characterization of a UV-B-inducible maize flavonol synthase
Falcone Ferreyra, María Lorena
FLAVONOL
SYNTHASE
title_short Cloning and characterization of a UV-B-inducible maize flavonol synthase
title_full Cloning and characterization of a UV-B-inducible maize flavonol synthase
title_fullStr Cloning and characterization of a UV-B-inducible maize flavonol synthase
title_full_unstemmed Cloning and characterization of a UV-B-inducible maize flavonol synthase
title_sort Cloning and characterization of a UV-B-inducible maize flavonol synthase
dc.creator.none.fl_str_mv Falcone Ferreyra, María Lorena
Rius, Sebastian Pablo
Emiliani, Julia
Pourcel, Lucille
Feller, Antje
Morohashi, Kengo
Casati, Paula
Grotewold, Erich
author Falcone Ferreyra, María Lorena
author_facet Falcone Ferreyra, María Lorena
Rius, Sebastian Pablo
Emiliani, Julia
Pourcel, Lucille
Feller, Antje
Morohashi, Kengo
Casati, Paula
Grotewold, Erich
author_role author
author2 Rius, Sebastian Pablo
Emiliani, Julia
Pourcel, Lucille
Feller, Antje
Morohashi, Kengo
Casati, Paula
Grotewold, Erich
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FLAVONOL
SYNTHASE
topic FLAVONOL
SYNTHASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flavonols are important compounds for male fertility in maize and other crops, and they also contribute to protecting plants from UV-B radiation. However, little continues to be known on how maize and other grasses synthesize flavonols, and how flavonol biosynthesis is regulated. By homology to an Arabidopsis flavonol synthase (FLS1), we cloned a maize gene encoding a product (ZmFLS1) capable of converting the dihydrokaempferol (DHK) and dihydroquercetin (DHQ) dihydroflavonols to the corresponding flavonols, kaempferol (K) and quercetin (Q). Moreover, ZmFLS1 partially complements the flavonol deficiency of Arabidopsis fls1 mutant and restores anthocyanin accumulation to normal levels. We demonstrate that ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. Indeed, using chromatin immunoprecipitation (ChIP) experiments, we demonstrate that ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes, revealing a similar control as for earlier steps in the maize flavonoid pathway. Highlighting the importance of flavonols in UV-B protection, we also show that ZmFLS1 is induced in maize seedlings by UV-B, and that this induction is in part mediated by the increased expression of the P1, B and PL1 regulators. Together, our results identify a key flavonoid biosynthetic enzyme so far missed in maize and other monocots, and illustrate mechanisms by which flavonol accumulation is controlled in maize.
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Rius, Sebastian Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Emiliani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Pourcel, Lucille. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
Fil: Feller, Antje. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
Fil: Morohashi, Kengo. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Grotewold, Erich. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology. Plant Biology; Estados Unidos
description Flavonols are important compounds for male fertility in maize and other crops, and they also contribute to protecting plants from UV-B radiation. However, little continues to be known on how maize and other grasses synthesize flavonols, and how flavonol biosynthesis is regulated. By homology to an Arabidopsis flavonol synthase (FLS1), we cloned a maize gene encoding a product (ZmFLS1) capable of converting the dihydrokaempferol (DHK) and dihydroquercetin (DHQ) dihydroflavonols to the corresponding flavonols, kaempferol (K) and quercetin (Q). Moreover, ZmFLS1 partially complements the flavonol deficiency of Arabidopsis fls1 mutant and restores anthocyanin accumulation to normal levels. We demonstrate that ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators. Indeed, using chromatin immunoprecipitation (ChIP) experiments, we demonstrate that ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes, revealing a similar control as for earlier steps in the maize flavonoid pathway. Highlighting the importance of flavonols in UV-B protection, we also show that ZmFLS1 is induced in maize seedlings by UV-B, and that this induction is in part mediated by the increased expression of the P1, B and PL1 regulators. Together, our results identify a key flavonoid biosynthetic enzyme so far missed in maize and other monocots, and illustrate mechanisms by which flavonol accumulation is controlled in maize.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276651
Falcone Ferreyra, María Lorena; Rius, Sebastian Pablo; Emiliani, Julia; Pourcel, Lucille; Feller, Antje; et al.; Cloning and characterization of a UV-B-inducible maize flavonol synthase; Wiley Blackwell Publishing, Inc; Plant Journal; 62; 1; 4-2010; 77-91
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276651
identifier_str_mv Falcone Ferreyra, María Lorena; Rius, Sebastian Pablo; Emiliani, Julia; Pourcel, Lucille; Feller, Antje; et al.; Cloning and characterization of a UV-B-inducible maize flavonol synthase; Wiley Blackwell Publishing, Inc; Plant Journal; 62; 1; 4-2010; 77-91
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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