Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli

Autores
Marani, Mariela Mirta; Perez, Luis Orlando; de Araujo, Alyne Rodrigues; Plácido, Alexandra; Sousa, Carla F.; Quelemes, Patrick Veras; Oliveira, Mayara; Gomes Alves, Ana G.; Pueta, Mariana; Gameiro, Paula; Tomás, Ana M.; Delerue Matos, Cristina; Eaton, Peter; Camperi, Silvia Andrea; Basso, Nestor Guillermo; de Souza de Almeida Leite, Jose Roberto
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 μM). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Perez, Luis Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico de Ciencias Sociales y Humanas; Argentina
Fil: de Araujo, Alyne Rodrigues. Universidade Federal do Piauí; Brasil
Fil: Plácido, Alexandra. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Sousa, Carla F.. Universidad de Porto; Portugal
Fil: Quelemes, Patrick Veras. Universidade Federal do Piauí; Brasil
Fil: Oliveira, Mayara. Universidade do Brasília; Brasil
Fil: Gomes Alves, Ana G.. Universidad de Porto; Portugal. Universidade Do Minho; Portugal
Fil: Pueta, Mariana. Instituto D/invest.en Biodiversidad y Medioambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Gameiro, Paula. Universidad de Porto; Portugal
Fil: Tomás, Ana M.. Universidad de Porto; Portugal
Fil: Delerue Matos, Cristina. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Eaton, Peter. Universidad de Porto; Portugal. Universidade Federal do Piauí; Brasil
Fil: Camperi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Basso, Nestor Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina
Fil: de Souza de Almeida Leite, Jose Roberto. Universidade Federal do Piauí; Brasil. Universidad de Porto; Portugal. Universidade do Brasília; Brasil
Materia
ANURA
ATOMIC FORCE MICROSCOPY
CDNA
CIRCULAR DICHROISM
SURFACE PLASMON RESONANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36933
Acceder
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spelling Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coliMarani, Mariela MirtaPerez, Luis Orlandode Araujo, Alyne RodriguesPlácido, AlexandraSousa, Carla F.Quelemes, Patrick VerasOliveira, MayaraGomes Alves, Ana G.Pueta, MarianaGameiro, PaulaTomás, Ana M.Delerue Matos, CristinaEaton, PeterCamperi, Silvia AndreaBasso, Nestor Guillermode Souza de Almeida Leite, Jose RobertoANURAATOMIC FORCE MICROSCOPYCDNACIRCULAR DICHROISMSURFACE PLASMON RESONANCEPatagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 μM). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Perez, Luis Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico de Ciencias Sociales y Humanas; ArgentinaFil: de Araujo, Alyne Rodrigues. Universidade Federal do Piauí; BrasilFil: Plácido, Alexandra. Instituto Superior de Engenharia do Instituto Politécnico do Porto; PortugalFil: Sousa, Carla F.. Universidad de Porto; PortugalFil: Quelemes, Patrick Veras. Universidade Federal do Piauí; BrasilFil: Oliveira, Mayara. Universidade do Brasília; BrasilFil: Gomes Alves, Ana G.. Universidad de Porto; Portugal. Universidade Do Minho; PortugalFil: Pueta, Mariana. Instituto D/invest.en Biodiversidad y Medioambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue; ArgentinaFil: Gameiro, Paula. Universidad de Porto; PortugalFil: Tomás, Ana M.. Universidad de Porto; PortugalFil: Delerue Matos, Cristina. Instituto Superior de Engenharia do Instituto Politécnico do Porto; PortugalFil: Eaton, Peter. Universidad de Porto; Portugal. Universidade Federal do Piauí; BrasilFil: Camperi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Basso, Nestor Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: de Souza de Almeida Leite, Jose Roberto. Universidade Federal do Piauí; Brasil. Universidad de Porto; Portugal. Universidade do Brasília; BrasilElsevier Science2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36933Marani, Mariela Mirta; Perez, Luis Orlando; de Araujo, Alyne Rodrigues; Plácido, Alexandra; Sousa, Carla F.; et al.; Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli; Elsevier Science; Gene; 605; 12-2016; 70-800378-11191879-0038CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378111916310204info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2016.12.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:22:24Zoai:ri.conicet.gov.ar:11336/36933instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:22:25.201CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
title Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
spellingShingle Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
Marani, Mariela Mirta
ANURA
ATOMIC FORCE MICROSCOPY
CDNA
CIRCULAR DICHROISM
SURFACE PLASMON RESONANCE
title_short Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
title_full Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
title_fullStr Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
title_full_unstemmed Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
title_sort Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
dc.creator.none.fl_str_mv Marani, Mariela Mirta
Perez, Luis Orlando
de Araujo, Alyne Rodrigues
Plácido, Alexandra
Sousa, Carla F.
Quelemes, Patrick Veras
Oliveira, Mayara
Gomes Alves, Ana G.
Pueta, Mariana
Gameiro, Paula
Tomás, Ana M.
Delerue Matos, Cristina
Eaton, Peter
Camperi, Silvia Andrea
Basso, Nestor Guillermo
de Souza de Almeida Leite, Jose Roberto
author Marani, Mariela Mirta
author_facet Marani, Mariela Mirta
Perez, Luis Orlando
de Araujo, Alyne Rodrigues
Plácido, Alexandra
Sousa, Carla F.
Quelemes, Patrick Veras
Oliveira, Mayara
Gomes Alves, Ana G.
Pueta, Mariana
Gameiro, Paula
Tomás, Ana M.
Delerue Matos, Cristina
Eaton, Peter
Camperi, Silvia Andrea
Basso, Nestor Guillermo
de Souza de Almeida Leite, Jose Roberto
author_role author
author2 Perez, Luis Orlando
de Araujo, Alyne Rodrigues
Plácido, Alexandra
Sousa, Carla F.
Quelemes, Patrick Veras
Oliveira, Mayara
Gomes Alves, Ana G.
Pueta, Mariana
Gameiro, Paula
Tomás, Ana M.
Delerue Matos, Cristina
Eaton, Peter
Camperi, Silvia Andrea
Basso, Nestor Guillermo
de Souza de Almeida Leite, Jose Roberto
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANURA
ATOMIC FORCE MICROSCOPY
CDNA
CIRCULAR DICHROISM
SURFACE PLASMON RESONANCE
topic ANURA
ATOMIC FORCE MICROSCOPY
CDNA
CIRCULAR DICHROISM
SURFACE PLASMON RESONANCE
dc.description.none.fl_txt_mv Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 μM). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Perez, Luis Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico de Ciencias Sociales y Humanas; Argentina
Fil: de Araujo, Alyne Rodrigues. Universidade Federal do Piauí; Brasil
Fil: Plácido, Alexandra. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Sousa, Carla F.. Universidad de Porto; Portugal
Fil: Quelemes, Patrick Veras. Universidade Federal do Piauí; Brasil
Fil: Oliveira, Mayara. Universidade do Brasília; Brasil
Fil: Gomes Alves, Ana G.. Universidad de Porto; Portugal. Universidade Do Minho; Portugal
Fil: Pueta, Mariana. Instituto D/invest.en Biodiversidad y Medioambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Gameiro, Paula. Universidad de Porto; Portugal
Fil: Tomás, Ana M.. Universidad de Porto; Portugal
Fil: Delerue Matos, Cristina. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Eaton, Peter. Universidad de Porto; Portugal. Universidade Federal do Piauí; Brasil
Fil: Camperi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Basso, Nestor Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina
Fil: de Souza de Almeida Leite, Jose Roberto. Universidade Federal do Piauí; Brasil. Universidad de Porto; Portugal. Universidade do Brasília; Brasil
description Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 μM). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36933
Marani, Mariela Mirta; Perez, Luis Orlando; de Araujo, Alyne Rodrigues; Plácido, Alexandra; Sousa, Carla F.; et al.; Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli; Elsevier Science; Gene; 605; 12-2016; 70-80
0378-1119
1879-0038
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36933
identifier_str_mv Marani, Mariela Mirta; Perez, Luis Orlando; de Araujo, Alyne Rodrigues; Plácido, Alexandra; Sousa, Carla F.; et al.; Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli; Elsevier Science; Gene; 605; 12-2016; 70-80
0378-1119
1879-0038
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378111916310204
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2016.12.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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