Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides

Autores
Mosquera, Jesus; Jimenez Balsa, Adrian; Dodero, Veronica Isabel; Vázquez, M. Eugenio; Mascareñas, José L.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the strategies used by nature to regulate gene expression relies on the stimuli controlled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While themonomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (5'-ATGA cg TCAT-3'), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (5-TCATcg ATGA-30'). As the dimerization mode can be efficiently controlled by appropriate external reagents, it is possible to reversibly drive the peptide to either DNA site in response to such specific inputs. This represents the firstexample of a designed molecule that can bind to more than one specific DNA sequence depending on changes in its environment.
Fil: Mosquera, Jesus. Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
Fil: Jimenez Balsa, Adrian. Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
Fil: Dodero, Veronica Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad de Santiago de Compostela; España
Fil: Vázquez, M. Eugenio . Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
Fil: Mascareñas, José L. . Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
Materia
BIOMOLECULAR
CONTROL
PEPTIDES
CIRCULAR DICHROISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5430
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5430
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptidesMosquera, JesusJimenez Balsa, AdrianDodero, Veronica IsabelVázquez, M. Eugenio Mascareñas, José L. BIOMOLECULARCONTROLPEPTIDESCIRCULAR DICHROISMhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1One of the strategies used by nature to regulate gene expression relies on the stimuli controlled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While themonomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (5'-ATGA cg TCAT-3'), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (5-TCATcg ATGA-30'). As the dimerization mode can be efficiently controlled by appropriate external reagents, it is possible to reversibly drive the peptide to either DNA site in response to such specific inputs. This represents the firstexample of a designed molecule that can bind to more than one specific DNA sequence depending on changes in its environment.Fil: Mosquera, Jesus. Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; EspañaFil: Jimenez Balsa, Adrian. Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; EspañaFil: Dodero, Veronica Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad de Santiago de Compostela; EspañaFil: Vázquez, M. Eugenio . Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; EspañaFil: Mascareñas, José L. . Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; EspañaNature2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5430Mosquera, Jesus; Jimenez Balsa, Adrian; Dodero, Veronica Isabel; Vázquez, M. Eugenio ; Mascareñas, José L. ; Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides; Nature; Nature Communications; 4; 1874; 5-2013; 1-82041-1723enginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/ncomms/journal/v4/n5/abs/ncomms2825.htmlinfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms2825info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:01Zoai:ri.conicet.gov.ar:11336/5430instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:01.316CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
title Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
spellingShingle Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
Mosquera, Jesus
BIOMOLECULAR
CONTROL
PEPTIDES
CIRCULAR DICHROISM
title_short Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
title_full Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
title_fullStr Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
title_full_unstemmed Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
title_sort Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
dc.creator.none.fl_str_mv Mosquera, Jesus
Jimenez Balsa, Adrian
Dodero, Veronica Isabel
Vázquez, M. Eugenio
Mascareñas, José L.
author Mosquera, Jesus
author_facet Mosquera, Jesus
Jimenez Balsa, Adrian
Dodero, Veronica Isabel
Vázquez, M. Eugenio
Mascareñas, José L.
author_role author
author2 Jimenez Balsa, Adrian
Dodero, Veronica Isabel
Vázquez, M. Eugenio
Mascareñas, José L.
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOMOLECULAR
CONTROL
PEPTIDES
CIRCULAR DICHROISM
topic BIOMOLECULAR
CONTROL
PEPTIDES
CIRCULAR DICHROISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv One of the strategies used by nature to regulate gene expression relies on the stimuli controlled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While themonomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (5'-ATGA cg TCAT-3'), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (5-TCATcg ATGA-30'). As the dimerization mode can be efficiently controlled by appropriate external reagents, it is possible to reversibly drive the peptide to either DNA site in response to such specific inputs. This represents the firstexample of a designed molecule that can bind to more than one specific DNA sequence depending on changes in its environment.
Fil: Mosquera, Jesus. Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
Fil: Jimenez Balsa, Adrian. Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
Fil: Dodero, Veronica Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad de Santiago de Compostela; España
Fil: Vázquez, M. Eugenio . Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
Fil: Mascareñas, José L. . Universidad de Santiago de Compostela. Facultad de Quimica. Departamento de Quimica Organica; España
description One of the strategies used by nature to regulate gene expression relies on the stimuli controlled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While themonomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (5'-ATGA cg TCAT-3'), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (5-TCATcg ATGA-30'). As the dimerization mode can be efficiently controlled by appropriate external reagents, it is possible to reversibly drive the peptide to either DNA site in response to such specific inputs. This represents the firstexample of a designed molecule that can bind to more than one specific DNA sequence depending on changes in its environment.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5430
Mosquera, Jesus; Jimenez Balsa, Adrian; Dodero, Veronica Isabel; Vázquez, M. Eugenio ; Mascareñas, José L. ; Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides; Nature; Nature Communications; 4; 1874; 5-2013; 1-8
2041-1723
url http://hdl.handle.net/11336/5430
identifier_str_mv Mosquera, Jesus; Jimenez Balsa, Adrian; Dodero, Veronica Isabel; Vázquez, M. Eugenio ; Mascareñas, José L. ; Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides; Nature; Nature Communications; 4; 1874; 5-2013; 1-8
2041-1723
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/ncomms/journal/v4/n5/abs/ncomms2825.html
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms2825
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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