Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats

Autores
González Arbeláez, Luisa Fernanda; Pérez Nuñez, Ignacio Adrián; Mosca, Susana Maria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to examine the effects of GSK-3 inhibitors compared with PRE and POS in spontaneously hypertensive rats (SHR). Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1-hour reperfusion (R); PRE: a cycle of 5 min GI and 10 minutes of R prior to 45 min GI; POS: three cycles of 30 sec GI/30 sec R at the start of R. Other hearts received lithium chloride (LiCl) or indirubin-3 -monoxime,5-iodo-(IMI) as GSK-3 inhibitors. All interventions reduced the infarct size observed in IC group. The expressions of P-GSK-3 and P-Akt decreased in IC and were restored after PRE, POS, and GSK-3 inhibitors treatments. An increase of cytosolic MnSOD activity and lipid peroxidation and a decrease of GSH content observed in IC hearts were attenuated in PRE, POS, and LiCl or IMI treatments. An increase of P-GSK-3/VDAC physical association and a partial recovery of mitochondrial permeability were also detected after interventions. These data show that, in SHR hearts, GSK-3 inhibitors mimic the cardioprotection afforded by PRE and POS and suggest that a decrease in mitochondrial permeability mediated by P-GSK-3/VDAC interaction is a crucial event.
Fil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Pérez Nuñez, Ignacio Adrián. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Materia
Gsk-3 Beta
Ischemic Preconditioning
Ischemic Postconditioning
Mitochondrial Swelling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11946
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11946
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive ratsGonzález Arbeláez, Luisa FernandaPérez Nuñez, Ignacio AdriánMosca, Susana MariaGsk-3 BetaIschemic PreconditioningIschemic PostconditioningMitochondrial Swellinghttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim of this study was to examine the effects of GSK-3 inhibitors compared with PRE and POS in spontaneously hypertensive rats (SHR). Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1-hour reperfusion (R); PRE: a cycle of 5 min GI and 10 minutes of R prior to 45 min GI; POS: three cycles of 30 sec GI/30 sec R at the start of R. Other hearts received lithium chloride (LiCl) or indirubin-3 -monoxime,5-iodo-(IMI) as GSK-3 inhibitors. All interventions reduced the infarct size observed in IC group. The expressions of P-GSK-3 and P-Akt decreased in IC and were restored after PRE, POS, and GSK-3 inhibitors treatments. An increase of cytosolic MnSOD activity and lipid peroxidation and a decrease of GSH content observed in IC hearts were attenuated in PRE, POS, and LiCl or IMI treatments. An increase of P-GSK-3/VDAC physical association and a partial recovery of mitochondrial permeability were also detected after interventions. These data show that, in SHR hearts, GSK-3 inhibitors mimic the cardioprotection afforded by PRE and POS and suggest that a decrease in mitochondrial permeability mediated by P-GSK-3/VDAC interaction is a crucial event.Fil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Pérez Nuñez, Ignacio Adrián. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaHindawi Publishing Corporation2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11946González Arbeláez, Luisa Fernanda; Pérez Nuñez, Ignacio Adrián; Mosca, Susana Maria; Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats; Hindawi Publishing Corporation; Journal Of Biomedicine And Biotechnology; 2013; 9-2013; 1-11; 3174562314-61332314-6141enginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2013/317456info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/317456/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:43Zoai:ri.conicet.gov.ar:11336/11946instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:43.379CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
title Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
spellingShingle Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
González Arbeláez, Luisa Fernanda
Gsk-3 Beta
Ischemic Preconditioning
Ischemic Postconditioning
Mitochondrial Swelling
title_short Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
title_full Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
title_fullStr Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
title_full_unstemmed Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
title_sort Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
dc.creator.none.fl_str_mv González Arbeláez, Luisa Fernanda
Pérez Nuñez, Ignacio Adrián
Mosca, Susana Maria
author González Arbeláez, Luisa Fernanda
author_facet González Arbeláez, Luisa Fernanda
Pérez Nuñez, Ignacio Adrián
Mosca, Susana Maria
author_role author
author2 Pérez Nuñez, Ignacio Adrián
Mosca, Susana Maria
author2_role author
author
dc.subject.none.fl_str_mv Gsk-3 Beta
Ischemic Preconditioning
Ischemic Postconditioning
Mitochondrial Swelling
topic Gsk-3 Beta
Ischemic Preconditioning
Ischemic Postconditioning
Mitochondrial Swelling
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this study was to examine the effects of GSK-3 inhibitors compared with PRE and POS in spontaneously hypertensive rats (SHR). Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1-hour reperfusion (R); PRE: a cycle of 5 min GI and 10 minutes of R prior to 45 min GI; POS: three cycles of 30 sec GI/30 sec R at the start of R. Other hearts received lithium chloride (LiCl) or indirubin-3 -monoxime,5-iodo-(IMI) as GSK-3 inhibitors. All interventions reduced the infarct size observed in IC group. The expressions of P-GSK-3 and P-Akt decreased in IC and were restored after PRE, POS, and GSK-3 inhibitors treatments. An increase of cytosolic MnSOD activity and lipid peroxidation and a decrease of GSH content observed in IC hearts were attenuated in PRE, POS, and LiCl or IMI treatments. An increase of P-GSK-3/VDAC physical association and a partial recovery of mitochondrial permeability were also detected after interventions. These data show that, in SHR hearts, GSK-3 inhibitors mimic the cardioprotection afforded by PRE and POS and suggest that a decrease in mitochondrial permeability mediated by P-GSK-3/VDAC interaction is a crucial event.
Fil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Pérez Nuñez, Ignacio Adrián. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
description The aim of this study was to examine the effects of GSK-3 inhibitors compared with PRE and POS in spontaneously hypertensive rats (SHR). Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1-hour reperfusion (R); PRE: a cycle of 5 min GI and 10 minutes of R prior to 45 min GI; POS: three cycles of 30 sec GI/30 sec R at the start of R. Other hearts received lithium chloride (LiCl) or indirubin-3 -monoxime,5-iodo-(IMI) as GSK-3 inhibitors. All interventions reduced the infarct size observed in IC group. The expressions of P-GSK-3 and P-Akt decreased in IC and were restored after PRE, POS, and GSK-3 inhibitors treatments. An increase of cytosolic MnSOD activity and lipid peroxidation and a decrease of GSH content observed in IC hearts were attenuated in PRE, POS, and LiCl or IMI treatments. An increase of P-GSK-3/VDAC physical association and a partial recovery of mitochondrial permeability were also detected after interventions. These data show that, in SHR hearts, GSK-3 inhibitors mimic the cardioprotection afforded by PRE and POS and suggest that a decrease in mitochondrial permeability mediated by P-GSK-3/VDAC interaction is a crucial event.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11946
González Arbeláez, Luisa Fernanda; Pérez Nuñez, Ignacio Adrián; Mosca, Susana Maria; Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats; Hindawi Publishing Corporation; Journal Of Biomedicine And Biotechnology; 2013; 9-2013; 1-11; 317456
2314-6133
2314-6141
url http://hdl.handle.net/11336/11946
identifier_str_mv González Arbeláez, Luisa Fernanda; Pérez Nuñez, Ignacio Adrián; Mosca, Susana Maria; Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats; Hindawi Publishing Corporation; Journal Of Biomedicine And Biotechnology; 2013; 9-2013; 1-11; 317456
2314-6133
2314-6141
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/317456
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/317456/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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