Engineering of the baculovirus expression system for optimized protein production

Autores
Martínez Solís, María; Herrero, Salvador; Targovnik, Alexandra Marisa
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multipleinsect pests. In addition to their application in pest control, baculoviruses have become a versatile and powerful eukaryotic vectorfor the production of large quantities of recombinant proteins for research and biomedical purposes. Since the first recombinantprotein was expressed in 1983 using the baculovirus expression system (BEVS), different strategies have been developed for thegeneration of recombinant viruses and to increase the stability, yield, and posttranslational modifications of recombinant proteins.In this review, we summarize the main methods and elements playing a role in the BEVS emphasizing recent progresses andfuture developments with respect to the main aspects involved in protein production using the BEVS.
Fil: Martínez Solís, María. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España
Fil: Herrero, Salvador. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España
Fil: Targovnik, Alexandra Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España
Materia
NUCLEOPOLYHEDROVIRUS
PROTEIN EXPRESSION
INSECT CELLS
INSECT BIOFACTORY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121575
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Engineering of the baculovirus expression system for optimized protein productionMartínez Solís, MaríaHerrero, SalvadorTargovnik, Alexandra MarisaNUCLEOPOLYHEDROVIRUSPROTEIN EXPRESSIONINSECT CELLSINSECT BIOFACTORYhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multipleinsect pests. In addition to their application in pest control, baculoviruses have become a versatile and powerful eukaryotic vectorfor the production of large quantities of recombinant proteins for research and biomedical purposes. Since the first recombinantprotein was expressed in 1983 using the baculovirus expression system (BEVS), different strategies have been developed for thegeneration of recombinant viruses and to increase the stability, yield, and posttranslational modifications of recombinant proteins.In this review, we summarize the main methods and elements playing a role in the BEVS emphasizing recent progresses andfuture developments with respect to the main aspects involved in protein production using the BEVS.Fil: Martínez Solís, María. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; EspañaFil: Herrero, Salvador. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; EspañaFil: Targovnik, Alexandra Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; EspañaSpringer2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121575Martínez Solís, María; Herrero, Salvador; Targovnik, Alexandra Marisa; Engineering of the baculovirus expression system for optimized protein production; Springer; Applied Microbiology and Biotechnology; 103; 1; 1-2019; 113-1230175-75981432-0614CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00253-018-9474-7info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-018-9474-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:39:39Zoai:ri.conicet.gov.ar:11336/121575instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:39:40.136CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Engineering of the baculovirus expression system for optimized protein production
title Engineering of the baculovirus expression system for optimized protein production
spellingShingle Engineering of the baculovirus expression system for optimized protein production
Martínez Solís, María
NUCLEOPOLYHEDROVIRUS
PROTEIN EXPRESSION
INSECT CELLS
INSECT BIOFACTORY
title_short Engineering of the baculovirus expression system for optimized protein production
title_full Engineering of the baculovirus expression system for optimized protein production
title_fullStr Engineering of the baculovirus expression system for optimized protein production
title_full_unstemmed Engineering of the baculovirus expression system for optimized protein production
title_sort Engineering of the baculovirus expression system for optimized protein production
dc.creator.none.fl_str_mv Martínez Solís, María
Herrero, Salvador
Targovnik, Alexandra Marisa
author Martínez Solís, María
author_facet Martínez Solís, María
Herrero, Salvador
Targovnik, Alexandra Marisa
author_role author
author2 Herrero, Salvador
Targovnik, Alexandra Marisa
author2_role author
author
dc.subject.none.fl_str_mv NUCLEOPOLYHEDROVIRUS
PROTEIN EXPRESSION
INSECT CELLS
INSECT BIOFACTORY
topic NUCLEOPOLYHEDROVIRUS
PROTEIN EXPRESSION
INSECT CELLS
INSECT BIOFACTORY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multipleinsect pests. In addition to their application in pest control, baculoviruses have become a versatile and powerful eukaryotic vectorfor the production of large quantities of recombinant proteins for research and biomedical purposes. Since the first recombinantprotein was expressed in 1983 using the baculovirus expression system (BEVS), different strategies have been developed for thegeneration of recombinant viruses and to increase the stability, yield, and posttranslational modifications of recombinant proteins.In this review, we summarize the main methods and elements playing a role in the BEVS emphasizing recent progresses andfuture developments with respect to the main aspects involved in protein production using the BEVS.
Fil: Martínez Solís, María. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España
Fil: Herrero, Salvador. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España
Fil: Targovnik, Alexandra Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España
description Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multipleinsect pests. In addition to their application in pest control, baculoviruses have become a versatile and powerful eukaryotic vectorfor the production of large quantities of recombinant proteins for research and biomedical purposes. Since the first recombinantprotein was expressed in 1983 using the baculovirus expression system (BEVS), different strategies have been developed for thegeneration of recombinant viruses and to increase the stability, yield, and posttranslational modifications of recombinant proteins.In this review, we summarize the main methods and elements playing a role in the BEVS emphasizing recent progresses andfuture developments with respect to the main aspects involved in protein production using the BEVS.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121575
Martínez Solís, María; Herrero, Salvador; Targovnik, Alexandra Marisa; Engineering of the baculovirus expression system for optimized protein production; Springer; Applied Microbiology and Biotechnology; 103; 1; 1-2019; 113-123
0175-7598
1432-0614
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121575
identifier_str_mv Martínez Solís, María; Herrero, Salvador; Targovnik, Alexandra Marisa; Engineering of the baculovirus expression system for optimized protein production; Springer; Applied Microbiology and Biotechnology; 103; 1; 1-2019; 113-123
0175-7598
1432-0614
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00253-018-9474-7
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-018-9474-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.071171

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