Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides

Autores
Peñalva, Daniel Alejandro; Wilke, Natalia; Maggio, Bruno; Aveldaño, Marta Isabel; Fanani, Maria Laura
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Molecular species of sphingomyelin (SM) with nonhydroxy (n) and 2-hydroxy (h) verylong-chain polyunsaturated fatty acids (n- and h-28:4, 30:5 and 32:5) abound in rat spermatogenic cells and spermatozoa. These SMs are located on the sperm head,where they are converted into the corresponding ceramides (Cer) after completion of the acrosomal reaction, as induced in vitro. The aim of this study was to look into the surface properties of these unique SM species and how these properties change by the SM→Cer conversion. After isolation by HPLC, these SMs were organized in Langmuir films and studied alone, in combination with different proportions of Cer, and during their conversion into Cer by sphingomyelinase. Compression isotherms for all six SMs under study were compatible with a liquid-expanded (LE) state and showed large molecular areas. Only the longest SMs (n-32:5 and h-32:5 SM) underwent a phase transition upon cooling. On the other hand, the abundant h-28:4 Cer exhibited an unusual highly compressible liquid-condensed (LC) phase, compatible with a high conformational freedom of Cer molecules but with the characteristic low diffusional properties of the LC phase. In mixed films of h-28:4 SM / h-28:4 Cer, the components showed a favourable mixing in the LE phase. The monolayer exhibited h-28:4 Cer-rich domains both in premixed films and when formed by the action of sphingomyelinase on pure h-28:4 SM films. In conclusion, while the SMs from sperm behaved in a way similar to shorter acylated SMs, the corresponding Cers showed atypical rheological properties that may be relevant for the membrane structural rearrangements that take place on the sperm head after completion of the acrosomal reaction.
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
Materia
2-Hydroxy Fatty Acids
Brewster Angle Microscopy
Single Particle Tracking
Langmuir Monolayers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7602
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7602
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramidesPeñalva, Daniel AlejandroWilke, NataliaMaggio, BrunoAveldaño, Marta IsabelFanani, Maria Laura2-Hydroxy Fatty AcidsBrewster Angle MicroscopySingle Particle TrackingLangmuir Monolayershttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Molecular species of sphingomyelin (SM) with nonhydroxy (n) and 2-hydroxy (h) verylong-chain polyunsaturated fatty acids (n- and h-28:4, 30:5 and 32:5) abound in rat spermatogenic cells and spermatozoa. These SMs are located on the sperm head,where they are converted into the corresponding ceramides (Cer) after completion of the acrosomal reaction, as induced in vitro. The aim of this study was to look into the surface properties of these unique SM species and how these properties change by the SM→Cer conversion. After isolation by HPLC, these SMs were organized in Langmuir films and studied alone, in combination with different proportions of Cer, and during their conversion into Cer by sphingomyelinase. Compression isotherms for all six SMs under study were compatible with a liquid-expanded (LE) state and showed large molecular areas. Only the longest SMs (n-32:5 and h-32:5 SM) underwent a phase transition upon cooling. On the other hand, the abundant h-28:4 Cer exhibited an unusual highly compressible liquid-condensed (LC) phase, compatible with a high conformational freedom of Cer molecules but with the characteristic low diffusional properties of the LC phase. In mixed films of h-28:4 SM / h-28:4 Cer, the components showed a favourable mixing in the LE phase. The monolayer exhibited h-28:4 Cer-rich domains both in premixed films and when formed by the action of sphingomyelinase on pure h-28:4 SM films. In conclusion, while the SMs from sperm behaved in a way similar to shorter acylated SMs, the corresponding Cers showed atypical rheological properties that may be relevant for the membrane structural rearrangements that take place on the sperm head after completion of the acrosomal reaction.Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); ArgentinaFil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); ArgentinaFil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); ArgentinaFil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); ArgentinaAmerican Chemical Society2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7602Peñalva, Daniel Alejandro; Wilke, Natalia; Maggio, Bruno; Aveldaño, Marta Isabel; Fanani, Maria Laura; Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides; American Chemical Society; Langmuir; 30; 15; 3-2014; 4385-43950743-7463enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/la500485xinfo:eu-repo/semantics/altIdentifier/doi/10.1021/la500485xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:39Zoai:ri.conicet.gov.ar:11336/7602instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:39.783CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
title Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
spellingShingle Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
Peñalva, Daniel Alejandro
2-Hydroxy Fatty Acids
Brewster Angle Microscopy
Single Particle Tracking
Langmuir Monolayers
title_short Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
title_full Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
title_fullStr Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
title_full_unstemmed Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
title_sort Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides
dc.creator.none.fl_str_mv Peñalva, Daniel Alejandro
Wilke, Natalia
Maggio, Bruno
Aveldaño, Marta Isabel
Fanani, Maria Laura
author Peñalva, Daniel Alejandro
author_facet Peñalva, Daniel Alejandro
Wilke, Natalia
Maggio, Bruno
Aveldaño, Marta Isabel
Fanani, Maria Laura
author_role author
author2 Wilke, Natalia
Maggio, Bruno
Aveldaño, Marta Isabel
Fanani, Maria Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv 2-Hydroxy Fatty Acids
Brewster Angle Microscopy
Single Particle Tracking
Langmuir Monolayers
topic 2-Hydroxy Fatty Acids
Brewster Angle Microscopy
Single Particle Tracking
Langmuir Monolayers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Molecular species of sphingomyelin (SM) with nonhydroxy (n) and 2-hydroxy (h) verylong-chain polyunsaturated fatty acids (n- and h-28:4, 30:5 and 32:5) abound in rat spermatogenic cells and spermatozoa. These SMs are located on the sperm head,where they are converted into the corresponding ceramides (Cer) after completion of the acrosomal reaction, as induced in vitro. The aim of this study was to look into the surface properties of these unique SM species and how these properties change by the SM→Cer conversion. After isolation by HPLC, these SMs were organized in Langmuir films and studied alone, in combination with different proportions of Cer, and during their conversion into Cer by sphingomyelinase. Compression isotherms for all six SMs under study were compatible with a liquid-expanded (LE) state and showed large molecular areas. Only the longest SMs (n-32:5 and h-32:5 SM) underwent a phase transition upon cooling. On the other hand, the abundant h-28:4 Cer exhibited an unusual highly compressible liquid-condensed (LC) phase, compatible with a high conformational freedom of Cer molecules but with the characteristic low diffusional properties of the LC phase. In mixed films of h-28:4 SM / h-28:4 Cer, the components showed a favourable mixing in the LE phase. The monolayer exhibited h-28:4 Cer-rich domains both in premixed films and when formed by the action of sphingomyelinase on pure h-28:4 SM films. In conclusion, while the SMs from sperm behaved in a way similar to shorter acylated SMs, the corresponding Cers showed atypical rheological properties that may be relevant for the membrane structural rearrangements that take place on the sperm head after completion of the acrosomal reaction.
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
description Molecular species of sphingomyelin (SM) with nonhydroxy (n) and 2-hydroxy (h) verylong-chain polyunsaturated fatty acids (n- and h-28:4, 30:5 and 32:5) abound in rat spermatogenic cells and spermatozoa. These SMs are located on the sperm head,where they are converted into the corresponding ceramides (Cer) after completion of the acrosomal reaction, as induced in vitro. The aim of this study was to look into the surface properties of these unique SM species and how these properties change by the SM→Cer conversion. After isolation by HPLC, these SMs were organized in Langmuir films and studied alone, in combination with different proportions of Cer, and during their conversion into Cer by sphingomyelinase. Compression isotherms for all six SMs under study were compatible with a liquid-expanded (LE) state and showed large molecular areas. Only the longest SMs (n-32:5 and h-32:5 SM) underwent a phase transition upon cooling. On the other hand, the abundant h-28:4 Cer exhibited an unusual highly compressible liquid-condensed (LC) phase, compatible with a high conformational freedom of Cer molecules but with the characteristic low diffusional properties of the LC phase. In mixed films of h-28:4 SM / h-28:4 Cer, the components showed a favourable mixing in the LE phase. The monolayer exhibited h-28:4 Cer-rich domains both in premixed films and when formed by the action of sphingomyelinase on pure h-28:4 SM films. In conclusion, while the SMs from sperm behaved in a way similar to shorter acylated SMs, the corresponding Cers showed atypical rheological properties that may be relevant for the membrane structural rearrangements that take place on the sperm head after completion of the acrosomal reaction.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7602
Peñalva, Daniel Alejandro; Wilke, Natalia; Maggio, Bruno; Aveldaño, Marta Isabel; Fanani, Maria Laura; Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides; American Chemical Society; Langmuir; 30; 15; 3-2014; 4385-4395
0743-7463
url http://hdl.handle.net/11336/7602
identifier_str_mv Peñalva, Daniel Alejandro; Wilke, Natalia; Maggio, Bruno; Aveldaño, Marta Isabel; Fanani, Maria Laura; Surface behavior of sphingomyelins with very long chain polyunsaturated fatty acids and effects of their conversion to ceramides; American Chemical Society; Langmuir; 30; 15; 3-2014; 4385-4395
0743-7463
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/la500485x
info:eu-repo/semantics/altIdentifier/doi/10.1021/la500485x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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