Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice

Autores
Salgueiro, María Jimena; Collia, Nicolas; Duran, Hebe Alicia; Palmieri, Mónica Alejandra; Medina, Vanina Araceli; Ughetti, Ricardo; Nicolini, Jorge Osvaldo; Zubillaga, Marcela Beatriz
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work was to evaluate the biological effects of the 32P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a 32Ppatch was prepared with Chromic 32P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in six groups. Four groups received the contact brachytherapy treatments using a scheme of a single session of 40 and 60 Gy (SD40 and SD60) and a scheme of two sessions of 40 and 60 Gy each (FD40 and FD60). The other two groups were used as controls of the single (CSD) and the fractionated (CFD) treatments. Radiation doses were estimated with equations derived from the MIRD DOSE scheme, and biologically effective doses (BED) were calculated according to equations derived from the linear-quadratic model. The endpoint to evaluate the treatments effects was tumor size after a follow-up period of 44 days. Finally, animals were sacrificed in order to get samples of all tumors for histological analysis and PCNA staining. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of control groups were higher than for treated groups, independently of the scheme and the radiation dose considered. This radioactive 32Psilicone-patch which is easy to prepare and use in the treatment of skin diseases, seems promising as a radioactive device for clinical use.
Fil: Salgueiro, María Jimena. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Collia, Nicolas. Universidad de Buenos Aires; Argentina
Fil: Duran, Hebe Alicia. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palmieri, Mónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín; Argentina. Universidad de Buenos Aires; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ughetti, Ricardo. No especifíca;
Fil: Nicolini, Jorge Osvaldo. No especifíca;
Fil: Zubillaga, Marcela Beatriz. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
32P
BRACHYTHERAPY
SKIN CANCER
PATCH
SENCAR MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157331
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/157331
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological effects of brachytherapy using a 32P-patch on the skin of Sencar miceSalgueiro, María JimenaCollia, NicolasDuran, Hebe AliciaPalmieri, Mónica AlejandraMedina, Vanina AraceliUghetti, RicardoNicolini, Jorge OsvaldoZubillaga, Marcela Beatriz32PBRACHYTHERAPYSKIN CANCERPATCHSENCAR MICEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work was to evaluate the biological effects of the 32P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a 32Ppatch was prepared with Chromic 32P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in six groups. Four groups received the contact brachytherapy treatments using a scheme of a single session of 40 and 60 Gy (SD40 and SD60) and a scheme of two sessions of 40 and 60 Gy each (FD40 and FD60). The other two groups were used as controls of the single (CSD) and the fractionated (CFD) treatments. Radiation doses were estimated with equations derived from the MIRD DOSE scheme, and biologically effective doses (BED) were calculated according to equations derived from the linear-quadratic model. The endpoint to evaluate the treatments effects was tumor size after a follow-up period of 44 days. Finally, animals were sacrificed in order to get samples of all tumors for histological analysis and PCNA staining. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of control groups were higher than for treated groups, independently of the scheme and the radiation dose considered. This radioactive 32Psilicone-patch which is easy to prepare and use in the treatment of skin diseases, seems promising as a radioactive device for clinical use.Fil: Salgueiro, María Jimena. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Collia, Nicolas. Universidad de Buenos Aires; ArgentinaFil: Duran, Hebe Alicia. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palmieri, Mónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín; Argentina. Universidad de Buenos Aires; ArgentinaFil: Medina, Vanina Araceli. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ughetti, Ricardo. No especifíca;Fil: Nicolini, Jorge Osvaldo. No especifíca;Fil: Zubillaga, Marcela Beatriz. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPergamon-Elsevier Science Ltd2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157331Salgueiro, María Jimena; Collia, Nicolas; Duran, Hebe Alicia; Palmieri, Mónica Alejandra; Medina, Vanina Araceli; et al.; Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice; Pergamon-Elsevier Science Ltd; Applied Radiation and Isotopes; 67; 10-2009; 1769-17740969-8043CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0969804309004060?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.apradiso.2009.05.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:14:37Zoai:ri.conicet.gov.ar:11336/157331instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:14:37.816CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
title Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
spellingShingle Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
Salgueiro, María Jimena
32P
BRACHYTHERAPY
SKIN CANCER
PATCH
SENCAR MICE
title_short Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
title_full Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
title_fullStr Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
title_full_unstemmed Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
title_sort Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice
dc.creator.none.fl_str_mv Salgueiro, María Jimena
Collia, Nicolas
Duran, Hebe Alicia
Palmieri, Mónica Alejandra
Medina, Vanina Araceli
Ughetti, Ricardo
Nicolini, Jorge Osvaldo
Zubillaga, Marcela Beatriz
author Salgueiro, María Jimena
author_facet Salgueiro, María Jimena
Collia, Nicolas
Duran, Hebe Alicia
Palmieri, Mónica Alejandra
Medina, Vanina Araceli
Ughetti, Ricardo
Nicolini, Jorge Osvaldo
Zubillaga, Marcela Beatriz
author_role author
author2 Collia, Nicolas
Duran, Hebe Alicia
Palmieri, Mónica Alejandra
Medina, Vanina Araceli
Ughetti, Ricardo
Nicolini, Jorge Osvaldo
Zubillaga, Marcela Beatriz
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 32P
BRACHYTHERAPY
SKIN CANCER
PATCH
SENCAR MICE
topic 32P
BRACHYTHERAPY
SKIN CANCER
PATCH
SENCAR MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work was to evaluate the biological effects of the 32P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a 32Ppatch was prepared with Chromic 32P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in six groups. Four groups received the contact brachytherapy treatments using a scheme of a single session of 40 and 60 Gy (SD40 and SD60) and a scheme of two sessions of 40 and 60 Gy each (FD40 and FD60). The other two groups were used as controls of the single (CSD) and the fractionated (CFD) treatments. Radiation doses were estimated with equations derived from the MIRD DOSE scheme, and biologically effective doses (BED) were calculated according to equations derived from the linear-quadratic model. The endpoint to evaluate the treatments effects was tumor size after a follow-up period of 44 days. Finally, animals were sacrificed in order to get samples of all tumors for histological analysis and PCNA staining. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of control groups were higher than for treated groups, independently of the scheme and the radiation dose considered. This radioactive 32Psilicone-patch which is easy to prepare and use in the treatment of skin diseases, seems promising as a radioactive device for clinical use.
Fil: Salgueiro, María Jimena. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Collia, Nicolas. Universidad de Buenos Aires; Argentina
Fil: Duran, Hebe Alicia. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palmieri, Mónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín; Argentina. Universidad de Buenos Aires; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ughetti, Ricardo. No especifíca;
Fil: Nicolini, Jorge Osvaldo. No especifíca;
Fil: Zubillaga, Marcela Beatriz. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work was to evaluate the biological effects of the 32P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a 32Ppatch was prepared with Chromic 32P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in six groups. Four groups received the contact brachytherapy treatments using a scheme of a single session of 40 and 60 Gy (SD40 and SD60) and a scheme of two sessions of 40 and 60 Gy each (FD40 and FD60). The other two groups were used as controls of the single (CSD) and the fractionated (CFD) treatments. Radiation doses were estimated with equations derived from the MIRD DOSE scheme, and biologically effective doses (BED) were calculated according to equations derived from the linear-quadratic model. The endpoint to evaluate the treatments effects was tumor size after a follow-up period of 44 days. Finally, animals were sacrificed in order to get samples of all tumors for histological analysis and PCNA staining. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of control groups were higher than for treated groups, independently of the scheme and the radiation dose considered. This radioactive 32Psilicone-patch which is easy to prepare and use in the treatment of skin diseases, seems promising as a radioactive device for clinical use.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157331
Salgueiro, María Jimena; Collia, Nicolas; Duran, Hebe Alicia; Palmieri, Mónica Alejandra; Medina, Vanina Araceli; et al.; Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice; Pergamon-Elsevier Science Ltd; Applied Radiation and Isotopes; 67; 10-2009; 1769-1774
0969-8043
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157331
identifier_str_mv Salgueiro, María Jimena; Collia, Nicolas; Duran, Hebe Alicia; Palmieri, Mónica Alejandra; Medina, Vanina Araceli; et al.; Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice; Pergamon-Elsevier Science Ltd; Applied Radiation and Isotopes; 67; 10-2009; 1769-1774
0969-8043
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0969804309004060?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apradiso.2009.05.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.075124

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