Non-Linear 3D Impedance Spectroscopy
- Autores
- Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface.
Fil: Zamora, Martín Lucas. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trujillo, Ricardo Matias. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Felice, Carmelo Jose. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Yeast
Bioimpedance
Non Linear
Biological - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/30528
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Non-Linear 3D Impedance SpectroscopyZamora, Martín LucasTrujillo, Ricardo MatiasFelice, Carmelo JoseYeastBioimpedanceNon LinearBiologicalhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface.Fil: Zamora, Martín Lucas. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trujillo, Ricardo Matias. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Felice, Carmelo Jose. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInstitute of Electrical and Electronics Engineers2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30528Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose; Non-Linear 3D Impedance Spectroscopy; Institute of Electrical and Electronics Engineers; IEEE Latin America Transactions; 12; 5; 8-2014; 895-8981548-0992CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1109/TLA.2014.6872902info:eu-repo/semantics/altIdentifier/url/http://ieeexplore.ieee.org/document/6872902/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:24Zoai:ri.conicet.gov.ar:11336/30528instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:24.379CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Non-Linear 3D Impedance Spectroscopy |
title |
Non-Linear 3D Impedance Spectroscopy |
spellingShingle |
Non-Linear 3D Impedance Spectroscopy Zamora, Martín Lucas Yeast Bioimpedance Non Linear Biological |
title_short |
Non-Linear 3D Impedance Spectroscopy |
title_full |
Non-Linear 3D Impedance Spectroscopy |
title_fullStr |
Non-Linear 3D Impedance Spectroscopy |
title_full_unstemmed |
Non-Linear 3D Impedance Spectroscopy |
title_sort |
Non-Linear 3D Impedance Spectroscopy |
dc.creator.none.fl_str_mv |
Zamora, Martín Lucas Trujillo, Ricardo Matias Felice, Carmelo Jose |
author |
Zamora, Martín Lucas |
author_facet |
Zamora, Martín Lucas Trujillo, Ricardo Matias Felice, Carmelo Jose |
author_role |
author |
author2 |
Trujillo, Ricardo Matias Felice, Carmelo Jose |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Yeast Bioimpedance Non Linear Biological |
topic |
Yeast Bioimpedance Non Linear Biological |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface. Fil: Zamora, Martín Lucas. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trujillo, Ricardo Matias. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Felice, Carmelo Jose. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/30528 Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose; Non-Linear 3D Impedance Spectroscopy; Institute of Electrical and Electronics Engineers; IEEE Latin America Transactions; 12; 5; 8-2014; 895-898 1548-0992 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/30528 |
identifier_str_mv |
Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose; Non-Linear 3D Impedance Spectroscopy; Institute of Electrical and Electronics Engineers; IEEE Latin America Transactions; 12; 5; 8-2014; 895-898 1548-0992 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1109/TLA.2014.6872902 info:eu-repo/semantics/altIdentifier/url/http://ieeexplore.ieee.org/document/6872902/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Electrical and Electronics Engineers |
publisher.none.fl_str_mv |
Institute of Electrical and Electronics Engineers |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614108416049152 |
score |
13.070432 |