Non-Linear 3D Impedance Spectroscopy

Autores
Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface.
Fil: Zamora, Martín Lucas. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trujillo, Ricardo Matias. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Felice, Carmelo Jose. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Yeast
Bioimpedance
Non Linear
Biological
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30528
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Non-Linear 3D Impedance SpectroscopyZamora, Martín LucasTrujillo, Ricardo MatiasFelice, Carmelo JoseYeastBioimpedanceNon LinearBiologicalhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface.Fil: Zamora, Martín Lucas. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trujillo, Ricardo Matias. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Felice, Carmelo Jose. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInstitute of Electrical and Electronics Engineers2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30528Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose; Non-Linear 3D Impedance Spectroscopy; Institute of Electrical and Electronics Engineers; IEEE Latin America Transactions; 12; 5; 8-2014; 895-8981548-0992CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1109/TLA.2014.6872902info:eu-repo/semantics/altIdentifier/url/http://ieeexplore.ieee.org/document/6872902/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:46:32Zoai:ri.conicet.gov.ar:11336/30528instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:46:33.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-Linear 3D Impedance Spectroscopy
title Non-Linear 3D Impedance Spectroscopy
spellingShingle Non-Linear 3D Impedance Spectroscopy
Zamora, Martín Lucas
Yeast
Bioimpedance
Non Linear
Biological
title_short Non-Linear 3D Impedance Spectroscopy
title_full Non-Linear 3D Impedance Spectroscopy
title_fullStr Non-Linear 3D Impedance Spectroscopy
title_full_unstemmed Non-Linear 3D Impedance Spectroscopy
title_sort Non-Linear 3D Impedance Spectroscopy
dc.creator.none.fl_str_mv Zamora, Martín Lucas
Trujillo, Ricardo Matias
Felice, Carmelo Jose
author Zamora, Martín Lucas
author_facet Zamora, Martín Lucas
Trujillo, Ricardo Matias
Felice, Carmelo Jose
author_role author
author2 Trujillo, Ricardo Matias
Felice, Carmelo Jose
author2_role author
author
dc.subject.none.fl_str_mv Yeast
Bioimpedance
Non Linear
Biological
topic Yeast
Bioimpedance
Non Linear
Biological
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface.
Fil: Zamora, Martín Lucas. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trujillo, Ricardo Matias. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Felice, Carmelo Jose. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Non-linear Dielectric spectroscopy of Biological suspensions was first proposed by the Woodward and Kell group. The above mentioned arose as a technique able to monitor the enzymes state placed in the cell membrane of the biological tissue. This technique is limited to such enzymes because an external low frequency electrical field notably disturbs the membrane's electrical entourage. This kind of interactions can be monitor if the system generates current harmonics. This phenomenon was theorized by Astumian and Robertson. Treo, on his work suggests that the harmonics are originated at the interface and altered by the microorganism presence. Then Ruiz developed a fast method to detect the global non-linearity of a biological suspension. Nevertheless, on these works, the non-linearity interference due to the electrode-electrolyte interface was not controlled. The observed response is the result of the interface modulation because of the microorganism presence. On our work, we employed a tripolar measurement cell. The working electrode (WE) is a gold disc, the counter electrode (CE) is made of stainless steel and the reference electrode (RE) is a standard Ag/AgCl electrode. 1.74 g of lyophilized Saccharomyces cerevisiae was dissolved in 90 ml of saline medium. A frequency (0.1 - 1000 Hz) and tension AC (0- 600 mV) sweep was carried out without DC polarization. The first 5 output current harmonics were monitored. Results would indicate that it was successfully possible to measure the non-linearity due to only the yeasts cells, without the non-linear interference of the electrode - electrolyte interface.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30528
Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose; Non-Linear 3D Impedance Spectroscopy; Institute of Electrical and Electronics Engineers; IEEE Latin America Transactions; 12; 5; 8-2014; 895-898
1548-0992
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30528
identifier_str_mv Zamora, Martín Lucas; Trujillo, Ricardo Matias; Felice, Carmelo Jose; Non-Linear 3D Impedance Spectroscopy; Institute of Electrical and Electronics Engineers; IEEE Latin America Transactions; 12; 5; 8-2014; 895-898
1548-0992
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1109/TLA.2014.6872902
info:eu-repo/semantics/altIdentifier/url/http://ieeexplore.ieee.org/document/6872902/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Institute of Electrical and Electronics Engineers
publisher.none.fl_str_mv Institute of Electrical and Electronics Engineers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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