Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus

Autores
Rodríguez Fermepin, Martin; Trinchero, Mariela Fernanda; Minetto, J.; Beltrán González, Andrea Natalia; Fernandez, Belisario Enrique
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Classical actions of the neurotrophin family are related to cellular survival anddifferentiation. Moreover, acute effects of neurotrophins have been reported. Althoughneurotrophins effects on synaptic transmission at central nervous system level have beenlargely studied, acute effects of neurotrophins on hypothalamic noradrenergic transmissionare still poorly understood. Thus, we have studied the effects of the neurotrophin familymembers nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) andneurotrophin-4 (NT-4) on norepinephrine (NE) neuronal uptake and its evoked release, aswell as the receptor and the intracellular pathways involved in these processes in rathypothalamus.Present results indicate that BDNF increased NE uptake and decreased its evoked releasethrough a mechanism that involve Trk B receptor and phospholipase C. Moreover, NT-4,also through the Trk B receptor, decreased NE uptake and its evoked release by activatingphosphatidylinositol 3-OH-kinase. These effects were observed in whole hypothalamus aswell as in the anterior hypothalamic zone. On the other hand, NGF did not modifynoradrenergic transmission.In conclusion, we showed for the first time that BDNF and NT-4 activate two differentintracellular signalling pathways through a Trk B receptor dependent mechanism.Furthermore, present findings support the hypothesis that BDNF and NT-4 acutely applied,could be considered as modulators of noradrenergic transmission and thus may regulatehypothalamic physiological as well as pathophysiological responses.
Fil: Rodríguez Fermepin, Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Trinchero, Mariela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Minetto, J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Beltrán González, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Fernandez, Belisario Enrique. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
NEUROTROFIC FACTORS
HIPOTHALAMUS
NOREPINEPHRINE
RAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157567
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/157567
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamusRodríguez Fermepin, MartinTrinchero, Mariela FernandaMinetto, J.Beltrán González, Andrea NataliaFernandez, Belisario EnriqueNEUROTROFIC FACTORSHIPOTHALAMUSNOREPINEPHRINERAThttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Classical actions of the neurotrophin family are related to cellular survival anddifferentiation. Moreover, acute effects of neurotrophins have been reported. Althoughneurotrophins effects on synaptic transmission at central nervous system level have beenlargely studied, acute effects of neurotrophins on hypothalamic noradrenergic transmissionare still poorly understood. Thus, we have studied the effects of the neurotrophin familymembers nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) andneurotrophin-4 (NT-4) on norepinephrine (NE) neuronal uptake and its evoked release, aswell as the receptor and the intracellular pathways involved in these processes in rathypothalamus.Present results indicate that BDNF increased NE uptake and decreased its evoked releasethrough a mechanism that involve Trk B receptor and phospholipase C. Moreover, NT-4,also through the Trk B receptor, decreased NE uptake and its evoked release by activatingphosphatidylinositol 3-OH-kinase. These effects were observed in whole hypothalamus aswell as in the anterior hypothalamic zone. On the other hand, NGF did not modifynoradrenergic transmission.In conclusion, we showed for the first time that BDNF and NT-4 activate two differentintracellular signalling pathways through a Trk B receptor dependent mechanism.Furthermore, present findings support the hypothesis that BDNF and NT-4 acutely applied,could be considered as modulators of noradrenergic transmission and thus may regulatehypothalamic physiological as well as pathophysiological responses.Fil: Rodríguez Fermepin, Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Trinchero, Mariela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Minetto, J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Beltrán González, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Fernandez, Belisario Enrique. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier2009-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157567Rodríguez Fermepin, Martin; Trinchero, Mariela Fernanda; Minetto, J.; Beltrán González, Andrea Natalia; Fernandez, Belisario Enrique; Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus; Elsevier; Neuropeptides; 43; 8-2009; 275-2820143-4179CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.npep.2009.06.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0143417909000663info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:46Zoai:ri.conicet.gov.ar:11336/157567instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:46.816CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
title Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
spellingShingle Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
Rodríguez Fermepin, Martin
NEUROTROFIC FACTORS
HIPOTHALAMUS
NOREPINEPHRINE
RAT
title_short Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
title_full Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
title_fullStr Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
title_full_unstemmed Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
title_sort Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus
dc.creator.none.fl_str_mv Rodríguez Fermepin, Martin
Trinchero, Mariela Fernanda
Minetto, J.
Beltrán González, Andrea Natalia
Fernandez, Belisario Enrique
author Rodríguez Fermepin, Martin
author_facet Rodríguez Fermepin, Martin
Trinchero, Mariela Fernanda
Minetto, J.
Beltrán González, Andrea Natalia
Fernandez, Belisario Enrique
author_role author
author2 Trinchero, Mariela Fernanda
Minetto, J.
Beltrán González, Andrea Natalia
Fernandez, Belisario Enrique
author2_role author
author
author
author
dc.subject.none.fl_str_mv NEUROTROFIC FACTORS
HIPOTHALAMUS
NOREPINEPHRINE
RAT
topic NEUROTROFIC FACTORS
HIPOTHALAMUS
NOREPINEPHRINE
RAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Classical actions of the neurotrophin family are related to cellular survival anddifferentiation. Moreover, acute effects of neurotrophins have been reported. Althoughneurotrophins effects on synaptic transmission at central nervous system level have beenlargely studied, acute effects of neurotrophins on hypothalamic noradrenergic transmissionare still poorly understood. Thus, we have studied the effects of the neurotrophin familymembers nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) andneurotrophin-4 (NT-4) on norepinephrine (NE) neuronal uptake and its evoked release, aswell as the receptor and the intracellular pathways involved in these processes in rathypothalamus.Present results indicate that BDNF increased NE uptake and decreased its evoked releasethrough a mechanism that involve Trk B receptor and phospholipase C. Moreover, NT-4,also through the Trk B receptor, decreased NE uptake and its evoked release by activatingphosphatidylinositol 3-OH-kinase. These effects were observed in whole hypothalamus aswell as in the anterior hypothalamic zone. On the other hand, NGF did not modifynoradrenergic transmission.In conclusion, we showed for the first time that BDNF and NT-4 activate two differentintracellular signalling pathways through a Trk B receptor dependent mechanism.Furthermore, present findings support the hypothesis that BDNF and NT-4 acutely applied,could be considered as modulators of noradrenergic transmission and thus may regulatehypothalamic physiological as well as pathophysiological responses.
Fil: Rodríguez Fermepin, Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Trinchero, Mariela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Minetto, J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Beltrán González, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Fernandez, Belisario Enrique. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Classical actions of the neurotrophin family are related to cellular survival anddifferentiation. Moreover, acute effects of neurotrophins have been reported. Althoughneurotrophins effects on synaptic transmission at central nervous system level have beenlargely studied, acute effects of neurotrophins on hypothalamic noradrenergic transmissionare still poorly understood. Thus, we have studied the effects of the neurotrophin familymembers nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) andneurotrophin-4 (NT-4) on norepinephrine (NE) neuronal uptake and its evoked release, aswell as the receptor and the intracellular pathways involved in these processes in rathypothalamus.Present results indicate that BDNF increased NE uptake and decreased its evoked releasethrough a mechanism that involve Trk B receptor and phospholipase C. Moreover, NT-4,also through the Trk B receptor, decreased NE uptake and its evoked release by activatingphosphatidylinositol 3-OH-kinase. These effects were observed in whole hypothalamus aswell as in the anterior hypothalamic zone. On the other hand, NGF did not modifynoradrenergic transmission.In conclusion, we showed for the first time that BDNF and NT-4 activate two differentintracellular signalling pathways through a Trk B receptor dependent mechanism.Furthermore, present findings support the hypothesis that BDNF and NT-4 acutely applied,could be considered as modulators of noradrenergic transmission and thus may regulatehypothalamic physiological as well as pathophysiological responses.
publishDate 2009
dc.date.none.fl_str_mv 2009-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157567
Rodríguez Fermepin, Martin; Trinchero, Mariela Fernanda; Minetto, J.; Beltrán González, Andrea Natalia; Fernandez, Belisario Enrique; Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus; Elsevier; Neuropeptides; 43; 8-2009; 275-282
0143-4179
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157567
identifier_str_mv Rodríguez Fermepin, Martin; Trinchero, Mariela Fernanda; Minetto, J.; Beltrán González, Andrea Natalia; Fernandez, Belisario Enrique; Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus; Elsevier; Neuropeptides; 43; 8-2009; 275-282
0143-4179
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.npep.2009.06.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0143417909000663
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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