Fluoxetine modulates norepinephrine contractile effect on rat vas deferens

Autores
Busch, Lucila; Wald, Miriam Ruth; Sterin, Leonor Josefina; Borda, Enri Santiago
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate whether the antidepressant drug fluoxetine could modify rat vas deferens response to norepinephrine (NE), and to compare its effect with that of desipramine and cocaine. Results showed that 10-5 M fluoxetine produced a super-sensibility of vas deferens to NE. This result was the same as those obtained for 10-6 M desipramine or cocaine. Since the effect was Na+- and Cl--dependent, an inhibitory mechanism of neuronal NE transport was suggested. Fluoxetine did not modify [3H]prazosin k(d) or B(max) in rat vas deferens, reinforcing the hypothesis of a pre-synaptic site of action. On the other hand fluoxetine inhibited NE maximal effect. This inhibitory effect could be related to an antagonism of calcium entry through the voltage-dependent calcium channel, since it was partially reverted by increasing calcium concentration and, besides, the drug was able to inhibit the calcium concentration-response curve also. Contractions induced by 5-hydroxytryptamine (5-HT) were not modified in the presence of fluoxetine. It is concluded that fluoxetine modulates rat vas deferens response to low NE concentrations in the same manner as the selective inhibitor of NE neuronal uptake desipramine. This peripheral effect could participate in the modulation of the male reproductive tract observed by these drugs when used in clinical trials.
Fil: Busch, Lucila. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Wald, Miriam Ruth. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Materia
Fluoxetine
Neuronal Uptake
Norepinephrine
Vas Deferens
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39146
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39146
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fluoxetine modulates norepinephrine contractile effect on rat vas deferensBusch, LucilaWald, Miriam RuthSterin, Leonor JosefinaBorda, Enri SantiagoFluoxetineNeuronal UptakeNorepinephrineVas Deferenshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim of this study was to evaluate whether the antidepressant drug fluoxetine could modify rat vas deferens response to norepinephrine (NE), and to compare its effect with that of desipramine and cocaine. Results showed that 10-5 M fluoxetine produced a super-sensibility of vas deferens to NE. This result was the same as those obtained for 10-6 M desipramine or cocaine. Since the effect was Na+- and Cl--dependent, an inhibitory mechanism of neuronal NE transport was suggested. Fluoxetine did not modify [3H]prazosin k(d) or B(max) in rat vas deferens, reinforcing the hypothesis of a pre-synaptic site of action. On the other hand fluoxetine inhibited NE maximal effect. This inhibitory effect could be related to an antagonism of calcium entry through the voltage-dependent calcium channel, since it was partially reverted by increasing calcium concentration and, besides, the drug was able to inhibit the calcium concentration-response curve also. Contractions induced by 5-hydroxytryptamine (5-HT) were not modified in the presence of fluoxetine. It is concluded that fluoxetine modulates rat vas deferens response to low NE concentrations in the same manner as the selective inhibitor of NE neuronal uptake desipramine. This peripheral effect could participate in the modulation of the male reproductive tract observed by these drugs when used in clinical trials.Fil: Busch, Lucila. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Wald, Miriam Ruth. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2000-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39146Busch, Lucila; Wald, Miriam Ruth; Sterin, Leonor Josefina; Borda, Enri Santiago; Fluoxetine modulates norepinephrine contractile effect on rat vas deferens; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 41; 1; 1-2000; 39-451043-6618CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1006/phrs.1999.0559info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1043661899905595info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:32Zoai:ri.conicet.gov.ar:11336/39146instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:32.373CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
title Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
spellingShingle Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
Busch, Lucila
Fluoxetine
Neuronal Uptake
Norepinephrine
Vas Deferens
title_short Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
title_full Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
title_fullStr Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
title_full_unstemmed Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
title_sort Fluoxetine modulates norepinephrine contractile effect on rat vas deferens
dc.creator.none.fl_str_mv Busch, Lucila
Wald, Miriam Ruth
Sterin, Leonor Josefina
Borda, Enri Santiago
author Busch, Lucila
author_facet Busch, Lucila
Wald, Miriam Ruth
Sterin, Leonor Josefina
Borda, Enri Santiago
author_role author
author2 Wald, Miriam Ruth
Sterin, Leonor Josefina
Borda, Enri Santiago
author2_role author
author
author
dc.subject.none.fl_str_mv Fluoxetine
Neuronal Uptake
Norepinephrine
Vas Deferens
topic Fluoxetine
Neuronal Uptake
Norepinephrine
Vas Deferens
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this study was to evaluate whether the antidepressant drug fluoxetine could modify rat vas deferens response to norepinephrine (NE), and to compare its effect with that of desipramine and cocaine. Results showed that 10-5 M fluoxetine produced a super-sensibility of vas deferens to NE. This result was the same as those obtained for 10-6 M desipramine or cocaine. Since the effect was Na+- and Cl--dependent, an inhibitory mechanism of neuronal NE transport was suggested. Fluoxetine did not modify [3H]prazosin k(d) or B(max) in rat vas deferens, reinforcing the hypothesis of a pre-synaptic site of action. On the other hand fluoxetine inhibited NE maximal effect. This inhibitory effect could be related to an antagonism of calcium entry through the voltage-dependent calcium channel, since it was partially reverted by increasing calcium concentration and, besides, the drug was able to inhibit the calcium concentration-response curve also. Contractions induced by 5-hydroxytryptamine (5-HT) were not modified in the presence of fluoxetine. It is concluded that fluoxetine modulates rat vas deferens response to low NE concentrations in the same manner as the selective inhibitor of NE neuronal uptake desipramine. This peripheral effect could participate in the modulation of the male reproductive tract observed by these drugs when used in clinical trials.
Fil: Busch, Lucila. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Wald, Miriam Ruth. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
description The aim of this study was to evaluate whether the antidepressant drug fluoxetine could modify rat vas deferens response to norepinephrine (NE), and to compare its effect with that of desipramine and cocaine. Results showed that 10-5 M fluoxetine produced a super-sensibility of vas deferens to NE. This result was the same as those obtained for 10-6 M desipramine or cocaine. Since the effect was Na+- and Cl--dependent, an inhibitory mechanism of neuronal NE transport was suggested. Fluoxetine did not modify [3H]prazosin k(d) or B(max) in rat vas deferens, reinforcing the hypothesis of a pre-synaptic site of action. On the other hand fluoxetine inhibited NE maximal effect. This inhibitory effect could be related to an antagonism of calcium entry through the voltage-dependent calcium channel, since it was partially reverted by increasing calcium concentration and, besides, the drug was able to inhibit the calcium concentration-response curve also. Contractions induced by 5-hydroxytryptamine (5-HT) were not modified in the presence of fluoxetine. It is concluded that fluoxetine modulates rat vas deferens response to low NE concentrations in the same manner as the selective inhibitor of NE neuronal uptake desipramine. This peripheral effect could participate in the modulation of the male reproductive tract observed by these drugs when used in clinical trials.
publishDate 2000
dc.date.none.fl_str_mv 2000-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39146
Busch, Lucila; Wald, Miriam Ruth; Sterin, Leonor Josefina; Borda, Enri Santiago; Fluoxetine modulates norepinephrine contractile effect on rat vas deferens; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 41; 1; 1-2000; 39-45
1043-6618
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39146
identifier_str_mv Busch, Lucila; Wald, Miriam Ruth; Sterin, Leonor Josefina; Borda, Enri Santiago; Fluoxetine modulates norepinephrine contractile effect on rat vas deferens; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 41; 1; 1-2000; 39-45
1043-6618
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1006/phrs.1999.0559
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1043661899905595
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269915676934144
score 13.13397

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