Colchicine-induced apoptosis daily rhythms in male and female suckling mice

Autores
González, Norma Viviana; Barbeito, Claudio Gustavo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition.
Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Materia
Apoptosis
Colchicine
Circadian rhythms
Intestinal crypts
Age
Gender
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244692

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network_name_str CONICET Digital (CONICET)
spelling Colchicine-induced apoptosis daily rhythms in male and female suckling miceGonzález, Norma VivianaBarbeito, Claudio GustavoApoptosisColchicineCircadian rhythmsIntestinal cryptsAgeGenderhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition.Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaNova Science Publishers2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244692González, Norma Viviana; Barbeito, Claudio Gustavo; Colchicine-induced apoptosis daily rhythms in male and female suckling mice; Nova Science Publishers; Animal Biology Journal; 2; 1; 10-2011; 11-201949-498XCONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:29Zoai:ri.conicet.gov.ar:11336/244692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:29.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Colchicine-induced apoptosis daily rhythms in male and female suckling mice
title Colchicine-induced apoptosis daily rhythms in male and female suckling mice
spellingShingle Colchicine-induced apoptosis daily rhythms in male and female suckling mice
González, Norma Viviana
Apoptosis
Colchicine
Circadian rhythms
Intestinal crypts
Age
Gender
title_short Colchicine-induced apoptosis daily rhythms in male and female suckling mice
title_full Colchicine-induced apoptosis daily rhythms in male and female suckling mice
title_fullStr Colchicine-induced apoptosis daily rhythms in male and female suckling mice
title_full_unstemmed Colchicine-induced apoptosis daily rhythms in male and female suckling mice
title_sort Colchicine-induced apoptosis daily rhythms in male and female suckling mice
dc.creator.none.fl_str_mv González, Norma Viviana
Barbeito, Claudio Gustavo
author González, Norma Viviana
author_facet González, Norma Viviana
Barbeito, Claudio Gustavo
author_role author
author2 Barbeito, Claudio Gustavo
author2_role author
dc.subject.none.fl_str_mv Apoptosis
Colchicine
Circadian rhythms
Intestinal crypts
Age
Gender
topic Apoptosis
Colchicine
Circadian rhythms
Intestinal crypts
Age
Gender
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition.
Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
description Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244692
González, Norma Viviana; Barbeito, Claudio Gustavo; Colchicine-induced apoptosis daily rhythms in male and female suckling mice; Nova Science Publishers; Animal Biology Journal; 2; 1; 10-2011; 11-20
1949-498X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244692
identifier_str_mv González, Norma Viviana; Barbeito, Claudio Gustavo; Colchicine-induced apoptosis daily rhythms in male and female suckling mice; Nova Science Publishers; Animal Biology Journal; 2; 1; 10-2011; 11-20
1949-498X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nova Science Publishers
publisher.none.fl_str_mv Nova Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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