Colchicine-induced apoptosis daily rhythms in male and female suckling mice
- Autores
- González, Norma Viviana; Barbeito, Claudio Gustavo
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition.
Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina - Materia
-
Apoptosis
Colchicine
Circadian rhythms
Intestinal crypts
Age
Gender - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/244692
Ver los metadatos del registro completo
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Colchicine-induced apoptosis daily rhythms in male and female suckling miceGonzález, Norma VivianaBarbeito, Claudio GustavoApoptosisColchicineCircadian rhythmsIntestinal cryptsAgeGenderhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition.Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaNova Science Publishers2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244692González, Norma Viviana; Barbeito, Claudio Gustavo; Colchicine-induced apoptosis daily rhythms in male and female suckling mice; Nova Science Publishers; Animal Biology Journal; 2; 1; 10-2011; 11-201949-498XCONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:29Zoai:ri.conicet.gov.ar:11336/244692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:29.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice |
title |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice |
spellingShingle |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice González, Norma Viviana Apoptosis Colchicine Circadian rhythms Intestinal crypts Age Gender |
title_short |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice |
title_full |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice |
title_fullStr |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice |
title_full_unstemmed |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice |
title_sort |
Colchicine-induced apoptosis daily rhythms in male and female suckling mice |
dc.creator.none.fl_str_mv |
González, Norma Viviana Barbeito, Claudio Gustavo |
author |
González, Norma Viviana |
author_facet |
González, Norma Viviana Barbeito, Claudio Gustavo |
author_role |
author |
author2 |
Barbeito, Claudio Gustavo |
author2_role |
author |
dc.subject.none.fl_str_mv |
Apoptosis Colchicine Circadian rhythms Intestinal crypts Age Gender |
topic |
Apoptosis Colchicine Circadian rhythms Intestinal crypts Age Gender |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition. Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina |
description |
Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/244692 González, Norma Viviana; Barbeito, Claudio Gustavo; Colchicine-induced apoptosis daily rhythms in male and female suckling mice; Nova Science Publishers; Animal Biology Journal; 2; 1; 10-2011; 11-20 1949-498X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/244692 |
identifier_str_mv |
González, Norma Viviana; Barbeito, Claudio Gustavo; Colchicine-induced apoptosis daily rhythms in male and female suckling mice; Nova Science Publishers; Animal Biology Journal; 2; 1; 10-2011; 11-20 1949-498X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Nova Science Publishers |
publisher.none.fl_str_mv |
Nova Science Publishers |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613974511845376 |
score |
13.070432 |