Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera

Autores
Giménez Martínez, Pablo Darian; Ramírez Ambrosi, Maria; Alonso Salces, Rosa Maria; Gallo, Blanca; Berrueta Luis, Angel; Maggi, Matías Daniel; Fuselli, Sandra Rosa
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apis mellifera populations are exposed to different biotic and abiotic stressors that affect bee survival. Paenibacillus larvae, the causative agent of American foulbrood, is one of the most important bacterial pathogens that affect bee health. In some countries, the use of antibiotics is the most common method for the prevention and treatment of P. larvae infected colonies, however this application can also increase the risk of occurrence of resistant strains. An ecological alternative is the use of vegetal extracts containing bioactive compounds, such as polyphenols. The aim of this study is to check the antimicrobial activity of phenolic extracts of apple pomace, from the cider industry, against P. larvae strains by the broth micro dilution method. In addition, the toxicity of the phenolic extracts on A. mellifera was verified using the complete exposure method. All extracts contain from 715.31 to 11348.16 μg gal/g DW, determined by Folin-Ciocalteu show antimicrobial activity against P. larvae genotype ERIC I, ranging from 23 μg/mL to 150 μg/mL. Toxicity assays of apple pomace extracts on adult bees exhibited a maximum mortality of 18% after 48h. This promising alternative will be used in the future to evaluate its toxicity at a field level.
Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Agencia Nacional de Promoción Científica y Tecnológica; Argentina
Fil: Ramírez Ambrosi, Maria. Universidad del País Vasco; España
Fil: Alonso Salces, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gallo, Blanca. Universidad del País Vasco; España
Fil: Berrueta Luis, Angel. Universidad del País Vasco; España
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
ANTIMICROBIAL ACTIVITY
APIS MELLIFERA
APPLE POMACE
PAENIBACILLUS LARVAE
PHENOLIC EXTRACT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142143
Acceder
id CONICETDig_19fff03989d90a4950363b2cc2adf72c
oai_identifier_str oai:ri.conicet.gov.ar:11336/142143
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis melliferaGiménez Martínez, Pablo DarianRamírez Ambrosi, MariaAlonso Salces, Rosa MariaGallo, BlancaBerrueta Luis, AngelMaggi, Matías DanielFuselli, Sandra RosaANTIMICROBIAL ACTIVITYAPIS MELLIFERAAPPLE POMACEPAENIBACILLUS LARVAEPHENOLIC EXTRACThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Apis mellifera populations are exposed to different biotic and abiotic stressors that affect bee survival. Paenibacillus larvae, the causative agent of American foulbrood, is one of the most important bacterial pathogens that affect bee health. In some countries, the use of antibiotics is the most common method for the prevention and treatment of P. larvae infected colonies, however this application can also increase the risk of occurrence of resistant strains. An ecological alternative is the use of vegetal extracts containing bioactive compounds, such as polyphenols. The aim of this study is to check the antimicrobial activity of phenolic extracts of apple pomace, from the cider industry, against P. larvae strains by the broth micro dilution method. In addition, the toxicity of the phenolic extracts on A. mellifera was verified using the complete exposure method. All extracts contain from 715.31 to 11348.16 μg gal/g DW, determined by Folin-Ciocalteu show antimicrobial activity against P. larvae genotype ERIC I, ranging from 23 μg/mL to 150 μg/mL. Toxicity assays of apple pomace extracts on adult bees exhibited a maximum mortality of 18% after 48h. This promising alternative will be used in the future to evaluate its toxicity at a field level.Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Agencia Nacional de Promoción Científica y Tecnológica; ArgentinaFil: Ramírez Ambrosi, Maria. Universidad del País Vasco; EspañaFil: Alonso Salces, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Gallo, Blanca. Universidad del País Vasco; EspañaFil: Berrueta Luis, Angel. Universidad del País Vasco; EspañaFil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaSciendo2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142143Giménez Martínez, Pablo Darian; Ramírez Ambrosi, Maria; Alonso Salces, Rosa Maria; Gallo, Blanca; Berrueta Luis, Angel; et al.; Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera; Sciendo; Journal of Apicultural Science; 64; 2; 8-2020; 199-2081643-4439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sciendo.com/article/10.2478/jas-2020-0016info:eu-repo/semantics/altIdentifier/doi/10.2478/JAS-2020-0016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:33Zoai:ri.conicet.gov.ar:11336/142143instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:33.613CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
title Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
spellingShingle Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
Giménez Martínez, Pablo Darian
ANTIMICROBIAL ACTIVITY
APIS MELLIFERA
APPLE POMACE
PAENIBACILLUS LARVAE
PHENOLIC EXTRACT
title_short Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
title_full Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
title_fullStr Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
title_full_unstemmed Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
title_sort Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
dc.creator.none.fl_str_mv Giménez Martínez, Pablo Darian
Ramírez Ambrosi, Maria
Alonso Salces, Rosa Maria
Gallo, Blanca
Berrueta Luis, Angel
Maggi, Matías Daniel
Fuselli, Sandra Rosa
author Giménez Martínez, Pablo Darian
author_facet Giménez Martínez, Pablo Darian
Ramírez Ambrosi, Maria
Alonso Salces, Rosa Maria
Gallo, Blanca
Berrueta Luis, Angel
Maggi, Matías Daniel
Fuselli, Sandra Rosa
author_role author
author2 Ramírez Ambrosi, Maria
Alonso Salces, Rosa Maria
Gallo, Blanca
Berrueta Luis, Angel
Maggi, Matías Daniel
Fuselli, Sandra Rosa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL ACTIVITY
APIS MELLIFERA
APPLE POMACE
PAENIBACILLUS LARVAE
PHENOLIC EXTRACT
topic ANTIMICROBIAL ACTIVITY
APIS MELLIFERA
APPLE POMACE
PAENIBACILLUS LARVAE
PHENOLIC EXTRACT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Apis mellifera populations are exposed to different biotic and abiotic stressors that affect bee survival. Paenibacillus larvae, the causative agent of American foulbrood, is one of the most important bacterial pathogens that affect bee health. In some countries, the use of antibiotics is the most common method for the prevention and treatment of P. larvae infected colonies, however this application can also increase the risk of occurrence of resistant strains. An ecological alternative is the use of vegetal extracts containing bioactive compounds, such as polyphenols. The aim of this study is to check the antimicrobial activity of phenolic extracts of apple pomace, from the cider industry, against P. larvae strains by the broth micro dilution method. In addition, the toxicity of the phenolic extracts on A. mellifera was verified using the complete exposure method. All extracts contain from 715.31 to 11348.16 μg gal/g DW, determined by Folin-Ciocalteu show antimicrobial activity against P. larvae genotype ERIC I, ranging from 23 μg/mL to 150 μg/mL. Toxicity assays of apple pomace extracts on adult bees exhibited a maximum mortality of 18% after 48h. This promising alternative will be used in the future to evaluate its toxicity at a field level.
Fil: Giménez Martínez, Pablo Darian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Agencia Nacional de Promoción Científica y Tecnológica; Argentina
Fil: Ramírez Ambrosi, Maria. Universidad del País Vasco; España
Fil: Alonso Salces, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gallo, Blanca. Universidad del País Vasco; España
Fil: Berrueta Luis, Angel. Universidad del País Vasco; España
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description Apis mellifera populations are exposed to different biotic and abiotic stressors that affect bee survival. Paenibacillus larvae, the causative agent of American foulbrood, is one of the most important bacterial pathogens that affect bee health. In some countries, the use of antibiotics is the most common method for the prevention and treatment of P. larvae infected colonies, however this application can also increase the risk of occurrence of resistant strains. An ecological alternative is the use of vegetal extracts containing bioactive compounds, such as polyphenols. The aim of this study is to check the antimicrobial activity of phenolic extracts of apple pomace, from the cider industry, against P. larvae strains by the broth micro dilution method. In addition, the toxicity of the phenolic extracts on A. mellifera was verified using the complete exposure method. All extracts contain from 715.31 to 11348.16 μg gal/g DW, determined by Folin-Ciocalteu show antimicrobial activity against P. larvae genotype ERIC I, ranging from 23 μg/mL to 150 μg/mL. Toxicity assays of apple pomace extracts on adult bees exhibited a maximum mortality of 18% after 48h. This promising alternative will be used in the future to evaluate its toxicity at a field level.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142143
Giménez Martínez, Pablo Darian; Ramírez Ambrosi, Maria; Alonso Salces, Rosa Maria; Gallo, Blanca; Berrueta Luis, Angel; et al.; Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera; Sciendo; Journal of Apicultural Science; 64; 2; 8-2020; 199-208
1643-4439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142143
identifier_str_mv Giménez Martínez, Pablo Darian; Ramírez Ambrosi, Maria; Alonso Salces, Rosa Maria; Gallo, Blanca; Berrueta Luis, Angel; et al.; Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera; Sciendo; Journal of Apicultural Science; 64; 2; 8-2020; 199-208
1643-4439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sciendo.com/article/10.2478/jas-2020-0016
info:eu-repo/semantics/altIdentifier/doi/10.2478/JAS-2020-0016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sciendo
publisher.none.fl_str_mv Sciendo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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