Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification

Autores
Carabajal, Maira Lia; Levin, Laura Noemi; Alberto, Edgardo Omar; Lechner, Bernardo Ernesto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Co-cultivation of mushroom species produce changes on fungal enzyme production and fructification that were not in-depth studied hitherto. This work was conducted with the aim of studying how the interactions between Pleurotus ostreatus and Pleurotus citrinopileatus in a dual culture affect the lignocellulolytic enzymes production, influence yield values and modify the morphological properties of basidiomata obtained. With this purpose monocultures of both species in a straw-based substrate were investigated, as well as dual cultures with 3 different spatial patterns (one fungus in the upper portion of the bag and the second in the bottom, the opposite spatial pattern, and a mix of spawn from both, scattered in each bag). Co-cultivation did not increase basidiomata production, moreover, it inhibited carpophore development of P. ostreatus in the mix, and decreased basidiomata stem?s length significantly. Biological efficiencies achieved in monocultures of P. ostreatus and P. citrinopileatus were 95.3% and 67.6% respectively. Different spatial patterns affected not only enzyme production but also mycelial yield. Cellulolytic and xylanolytic activities detected increased significantly when P. ostreatus was inoculated in the upper portion of the bag and P. citrinopileatus in the bottom part, but co-cultivation did not stimulate ligninolytic enzyme production. The highest laccase (8.2 U/g) and Mn-peroxidase (7.5 U/g) activities were detected in monocultures of P. ostreatus, in coincidence with maximum lignin degradation (28%).
Fil: Carabajal, Maira Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
CO-CULTIVATION
SPECIES INTERACTION
LIGNOCELLULOLYTIC ENZYMES
MUSHROOM YIELD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268648
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268648
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructificationCarabajal, Maira LiaLevin, Laura NoemiAlberto, Edgardo OmarLechner, Bernardo ErnestoCO-CULTIVATIONSPECIES INTERACTIONLIGNOCELLULOLYTIC ENZYMESMUSHROOM YIELDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Co-cultivation of mushroom species produce changes on fungal enzyme production and fructification that were not in-depth studied hitherto. This work was conducted with the aim of studying how the interactions between Pleurotus ostreatus and Pleurotus citrinopileatus in a dual culture affect the lignocellulolytic enzymes production, influence yield values and modify the morphological properties of basidiomata obtained. With this purpose monocultures of both species in a straw-based substrate were investigated, as well as dual cultures with 3 different spatial patterns (one fungus in the upper portion of the bag and the second in the bottom, the opposite spatial pattern, and a mix of spawn from both, scattered in each bag). Co-cultivation did not increase basidiomata production, moreover, it inhibited carpophore development of P. ostreatus in the mix, and decreased basidiomata stem?s length significantly. Biological efficiencies achieved in monocultures of P. ostreatus and P. citrinopileatus were 95.3% and 67.6% respectively. Different spatial patterns affected not only enzyme production but also mycelial yield. Cellulolytic and xylanolytic activities detected increased significantly when P. ostreatus was inoculated in the upper portion of the bag and P. citrinopileatus in the bottom part, but co-cultivation did not stimulate ligninolytic enzyme production. The highest laccase (8.2 U/g) and Mn-peroxidase (7.5 U/g) activities were detected in monocultures of P. ostreatus, in coincidence with maximum lignin degradation (28%).Fil: Carabajal, Maira Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaElsevier2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268648Carabajal, Maira Lia; Levin, Laura Noemi; Alberto, Edgardo Omar; Lechner, Bernardo Ernesto; Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification; Elsevier; International Biodeterioration and Biodegradation; 66; 1; 1-2012; 71-760964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830511002290info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2011.11.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:00Zoai:ri.conicet.gov.ar:11336/268648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:01.188CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
title Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
spellingShingle Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
Carabajal, Maira Lia
CO-CULTIVATION
SPECIES INTERACTION
LIGNOCELLULOLYTIC ENZYMES
MUSHROOM YIELD
title_short Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
title_full Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
title_fullStr Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
title_full_unstemmed Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
title_sort Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification
dc.creator.none.fl_str_mv Carabajal, Maira Lia
Levin, Laura Noemi
Alberto, Edgardo Omar
Lechner, Bernardo Ernesto
author Carabajal, Maira Lia
author_facet Carabajal, Maira Lia
Levin, Laura Noemi
Alberto, Edgardo Omar
Lechner, Bernardo Ernesto
author_role author
author2 Levin, Laura Noemi
Alberto, Edgardo Omar
Lechner, Bernardo Ernesto
author2_role author
author
author
dc.subject.none.fl_str_mv CO-CULTIVATION
SPECIES INTERACTION
LIGNOCELLULOLYTIC ENZYMES
MUSHROOM YIELD
topic CO-CULTIVATION
SPECIES INTERACTION
LIGNOCELLULOLYTIC ENZYMES
MUSHROOM YIELD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Co-cultivation of mushroom species produce changes on fungal enzyme production and fructification that were not in-depth studied hitherto. This work was conducted with the aim of studying how the interactions between Pleurotus ostreatus and Pleurotus citrinopileatus in a dual culture affect the lignocellulolytic enzymes production, influence yield values and modify the morphological properties of basidiomata obtained. With this purpose monocultures of both species in a straw-based substrate were investigated, as well as dual cultures with 3 different spatial patterns (one fungus in the upper portion of the bag and the second in the bottom, the opposite spatial pattern, and a mix of spawn from both, scattered in each bag). Co-cultivation did not increase basidiomata production, moreover, it inhibited carpophore development of P. ostreatus in the mix, and decreased basidiomata stem?s length significantly. Biological efficiencies achieved in monocultures of P. ostreatus and P. citrinopileatus were 95.3% and 67.6% respectively. Different spatial patterns affected not only enzyme production but also mycelial yield. Cellulolytic and xylanolytic activities detected increased significantly when P. ostreatus was inoculated in the upper portion of the bag and P. citrinopileatus in the bottom part, but co-cultivation did not stimulate ligninolytic enzyme production. The highest laccase (8.2 U/g) and Mn-peroxidase (7.5 U/g) activities were detected in monocultures of P. ostreatus, in coincidence with maximum lignin degradation (28%).
Fil: Carabajal, Maira Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description Co-cultivation of mushroom species produce changes on fungal enzyme production and fructification that were not in-depth studied hitherto. This work was conducted with the aim of studying how the interactions between Pleurotus ostreatus and Pleurotus citrinopileatus in a dual culture affect the lignocellulolytic enzymes production, influence yield values and modify the morphological properties of basidiomata obtained. With this purpose monocultures of both species in a straw-based substrate were investigated, as well as dual cultures with 3 different spatial patterns (one fungus in the upper portion of the bag and the second in the bottom, the opposite spatial pattern, and a mix of spawn from both, scattered in each bag). Co-cultivation did not increase basidiomata production, moreover, it inhibited carpophore development of P. ostreatus in the mix, and decreased basidiomata stem?s length significantly. Biological efficiencies achieved in monocultures of P. ostreatus and P. citrinopileatus were 95.3% and 67.6% respectively. Different spatial patterns affected not only enzyme production but also mycelial yield. Cellulolytic and xylanolytic activities detected increased significantly when P. ostreatus was inoculated in the upper portion of the bag and P. citrinopileatus in the bottom part, but co-cultivation did not stimulate ligninolytic enzyme production. The highest laccase (8.2 U/g) and Mn-peroxidase (7.5 U/g) activities were detected in monocultures of P. ostreatus, in coincidence with maximum lignin degradation (28%).
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268648
Carabajal, Maira Lia; Levin, Laura Noemi; Alberto, Edgardo Omar; Lechner, Bernardo Ernesto; Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification; Elsevier; International Biodeterioration and Biodegradation; 66; 1; 1-2012; 71-76
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268648
identifier_str_mv Carabajal, Maira Lia; Levin, Laura Noemi; Alberto, Edgardo Omar; Lechner, Bernardo Ernesto; Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification; Elsevier; International Biodeterioration and Biodegradation; 66; 1; 1-2012; 71-76
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830511002290
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2011.11.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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