Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol

Autores
Regiart, Daniel Matias Gaston; Seia, Marco Abel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe an electrochemical immunosensor for the determination of the growth promoter α-zearalanol in bovine serum. The sensing scheme is based on a nanocomposite consisting of gold nanoparticles electrodeposited on multi-walled carbon nanotubes that were modified with poly(vinylpyridine) through in-situ polymerization. The electrodeposition of the gold nanoparticles enlarges the surface available for immobilization of antibodies against α-zearalanol. The nanocomposite film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. The calibration plot has a linear response in the concentrations range from 0.05 to 50 ng mL−1, and the detection limit is 16 pg mL−1. The time required for analysis is 12 min only which compares quite favorably with the time (90 min) required by the conventional ELISA. The method exhibits good selectivity, stability and reproducibility for detecting α-zearalanol in the livestock production.
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Seia, Marco Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Materia
Electrochemical
Immunosensor
Nanocomposite
Α-Zearalanol
Bovine Serum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5786
Acceder
id CONICETDig_18c52bc871a96e5680386189044b19bc
oai_identifier_str oai:ri.conicet.gov.ar:11336/5786
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanolRegiart, Daniel Matias GastonSeia, Marco AbelMessina, Germán AlejandroBertolino, Franco AdriánRaba, JulioElectrochemicalImmunosensorNanocompositeΑ-ZearalanolBovine Serumhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We describe an electrochemical immunosensor for the determination of the growth promoter α-zearalanol in bovine serum. The sensing scheme is based on a nanocomposite consisting of gold nanoparticles electrodeposited on multi-walled carbon nanotubes that were modified with poly(vinylpyridine) through in-situ polymerization. The electrodeposition of the gold nanoparticles enlarges the surface available for immobilization of antibodies against α-zearalanol. The nanocomposite film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. The calibration plot has a linear response in the concentrations range from 0.05 to 50 ng mL−1, and the detection limit is 16 pg mL−1. The time required for analysis is 12 min only which compares quite favorably with the time (90 min) required by the conventional ELISA. The method exhibits good selectivity, stability and reproducibility for detecting α-zearalanol in the livestock production.Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Seia, Marco Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaSpringer Wien2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5786Regiart, Daniel Matias Gaston; Seia, Marco Abel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol; Springer Wien; Mikrochimica Acta; 182; 3-4; 9-2014; 531-5380026-3672enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00604-014-1355-xinfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1007/s00604-014-1355-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:26Zoai:ri.conicet.gov.ar:11336/5786instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:26.699CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
title Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
spellingShingle Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
Regiart, Daniel Matias Gaston
Electrochemical
Immunosensor
Nanocomposite
Α-Zearalanol
Bovine Serum
title_short Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
title_full Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
title_fullStr Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
title_full_unstemmed Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
title_sort Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol
dc.creator.none.fl_str_mv Regiart, Daniel Matias Gaston
Seia, Marco Abel
Messina, Germán Alejandro
Bertolino, Franco Adrián
Raba, Julio
author Regiart, Daniel Matias Gaston
author_facet Regiart, Daniel Matias Gaston
Seia, Marco Abel
Messina, Germán Alejandro
Bertolino, Franco Adrián
Raba, Julio
author_role author
author2 Seia, Marco Abel
Messina, Germán Alejandro
Bertolino, Franco Adrián
Raba, Julio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Electrochemical
Immunosensor
Nanocomposite
Α-Zearalanol
Bovine Serum
topic Electrochemical
Immunosensor
Nanocomposite
Α-Zearalanol
Bovine Serum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We describe an electrochemical immunosensor for the determination of the growth promoter α-zearalanol in bovine serum. The sensing scheme is based on a nanocomposite consisting of gold nanoparticles electrodeposited on multi-walled carbon nanotubes that were modified with poly(vinylpyridine) through in-situ polymerization. The electrodeposition of the gold nanoparticles enlarges the surface available for immobilization of antibodies against α-zearalanol. The nanocomposite film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. The calibration plot has a linear response in the concentrations range from 0.05 to 50 ng mL−1, and the detection limit is 16 pg mL−1. The time required for analysis is 12 min only which compares quite favorably with the time (90 min) required by the conventional ELISA. The method exhibits good selectivity, stability and reproducibility for detecting α-zearalanol in the livestock production.
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Seia, Marco Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
description We describe an electrochemical immunosensor for the determination of the growth promoter α-zearalanol in bovine serum. The sensing scheme is based on a nanocomposite consisting of gold nanoparticles electrodeposited on multi-walled carbon nanotubes that were modified with poly(vinylpyridine) through in-situ polymerization. The electrodeposition of the gold nanoparticles enlarges the surface available for immobilization of antibodies against α-zearalanol. The nanocomposite film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. The calibration plot has a linear response in the concentrations range from 0.05 to 50 ng mL−1, and the detection limit is 16 pg mL−1. The time required for analysis is 12 min only which compares quite favorably with the time (90 min) required by the conventional ELISA. The method exhibits good selectivity, stability and reproducibility for detecting α-zearalanol in the livestock production.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5786
Regiart, Daniel Matias Gaston; Seia, Marco Abel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol; Springer Wien; Mikrochimica Acta; 182; 3-4; 9-2014; 531-538
0026-3672
url http://hdl.handle.net/11336/5786
identifier_str_mv Regiart, Daniel Matias Gaston; Seia, Marco Abel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol; Springer Wien; Mikrochimica Acta; 182; 3-4; 9-2014; 531-538
0026-3672
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00604-014-1355-x
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00604-014-1355-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Wien
publisher.none.fl_str_mv Springer Wien
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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