Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval

Autores
Fraiman Borrazás, Daniel Edmundo; Pandolfi, Julieta Belen; S Dargan; Parker, Peter J.; Ponce Dawson, Silvina Martha
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
Fil: Fraiman Borrazás, Daniel Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Pandolfi, Julieta Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: S Dargan. University of California at Irvine; Estados Unidos
Fil: Parker, Peter J.. University of California at Irvine; Estados Unidos
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
Calcium Dynamics
Puff Events
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71621
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff intervalFraiman Borrazás, Daniel EdmundoPandolfi, Julieta BelenS DarganParker, Peter J.Ponce Dawson, Silvina MarthaCalcium DynamicsPuff Eventshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.Fil: Fraiman Borrazás, Daniel Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Pandolfi, Julieta Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: S Dargan. University of California at Irvine; Estados UnidosFil: Parker, Peter J.. University of California at Irvine; Estados UnidosFil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaCell Press2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71621Fraiman Borrazás, Daniel Edmundo; Pandolfi, Julieta Belen; S Dargan; Parker, Peter J.; Ponce Dawson, Silvina Martha; Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval; Cell Press; Biophysical Journal; 90; 11; 12-2006; 3897-39070006-3495CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1529/biophysj.105.075911info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:09:40Zoai:ri.conicet.gov.ar:11336/71621instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:09:40.308CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
spellingShingle Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
Fraiman Borrazás, Daniel Edmundo
Calcium Dynamics
Puff Events
title_short Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_full Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_fullStr Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_full_unstemmed Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_sort Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
dc.creator.none.fl_str_mv Fraiman Borrazás, Daniel Edmundo
Pandolfi, Julieta Belen
S Dargan
Parker, Peter J.
Ponce Dawson, Silvina Martha
author Fraiman Borrazás, Daniel Edmundo
author_facet Fraiman Borrazás, Daniel Edmundo
Pandolfi, Julieta Belen
S Dargan
Parker, Peter J.
Ponce Dawson, Silvina Martha
author_role author
author2 Pandolfi, Julieta Belen
S Dargan
Parker, Peter J.
Ponce Dawson, Silvina Martha
author2_role author
author
author
author
dc.subject.none.fl_str_mv Calcium Dynamics
Puff Events
topic Calcium Dynamics
Puff Events
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
Fil: Fraiman Borrazás, Daniel Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Pandolfi, Julieta Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: S Dargan. University of California at Irvine; Estados Unidos
Fil: Parker, Peter J.. University of California at Irvine; Estados Unidos
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71621
Fraiman Borrazás, Daniel Edmundo; Pandolfi, Julieta Belen; S Dargan; Parker, Peter J.; Ponce Dawson, Silvina Martha; Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval; Cell Press; Biophysical Journal; 90; 11; 12-2006; 3897-3907
0006-3495
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71621
identifier_str_mv Fraiman Borrazás, Daniel Edmundo; Pandolfi, Julieta Belen; S Dargan; Parker, Peter J.; Ponce Dawson, Silvina Martha; Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval; Cell Press; Biophysical Journal; 90; 11; 12-2006; 3897-3907
0006-3495
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1529/biophysj.105.075911
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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