Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval

Autores
Fraiman, D.; Pando, B.; Dargan, S.; Parker, I.; Dawson, S.P.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
Fil:Dawson, S.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Biophys. J. 2006;90(11):3897-3907
Materia
1,4,5 triphosphate
calcium
phosphate
unclassified drug
animal cell
article
controlled study
dynamics
endoplasmic reticulum
fluorescence
Kolmogorov Smirnov test
muscle cell
nonhuman
oocyte
Xenopus laevis
Neptunia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00063495_v90_n11_p3897_Fraiman

id BDUBAFCEN_152630ae90594bba06c29dc96399c474
oai_identifier_str paperaa:paper_00063495_v90_n11_p3897_Fraiman
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff intervalFraiman, D.Pando, B.Dargan, S.Parker, I.Dawson, S.P.1,4,5 triphosphatecalciumphosphateunclassified druganimal cellarticlecontrolled studydynamicsendoplasmic reticulumfluorescenceKolmogorov Smirnov testmuscle cellnonhumanoocyteXenopus laevisNeptuniaPuffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.Fil:Dawson, S.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00063495_v90_n11_p3897_FraimanBiophys. J. 2006;90(11):3897-3907reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:54Zpaperaa:paper_00063495_v90_n11_p3897_FraimanInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:56.035Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
spellingShingle Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
Fraiman, D.
1,4,5 triphosphate
calcium
phosphate
unclassified drug
animal cell
article
controlled study
dynamics
endoplasmic reticulum
fluorescence
Kolmogorov Smirnov test
muscle cell
nonhuman
oocyte
Xenopus laevis
Neptunia
title_short Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_full Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_fullStr Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_full_unstemmed Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
title_sort Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
dc.creator.none.fl_str_mv Fraiman, D.
Pando, B.
Dargan, S.
Parker, I.
Dawson, S.P.
author Fraiman, D.
author_facet Fraiman, D.
Pando, B.
Dargan, S.
Parker, I.
Dawson, S.P.
author_role author
author2 Pando, B.
Dargan, S.
Parker, I.
Dawson, S.P.
author2_role author
author
author
author
dc.subject.none.fl_str_mv 1,4,5 triphosphate
calcium
phosphate
unclassified drug
animal cell
article
controlled study
dynamics
endoplasmic reticulum
fluorescence
Kolmogorov Smirnov test
muscle cell
nonhuman
oocyte
Xenopus laevis
Neptunia
topic 1,4,5 triphosphate
calcium
phosphate
unclassified drug
animal cell
article
controlled study
dynamics
endoplasmic reticulum
fluorescence
Kolmogorov Smirnov test
muscle cell
nonhuman
oocyte
Xenopus laevis
Neptunia
dc.description.none.fl_txt_mv Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
Fil:Dawson, S.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00063495_v90_n11_p3897_Fraiman
url http://hdl.handle.net/20.500.12110/paper_00063495_v90_n11_p3897_Fraiman
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biophys. J. 2006;90(11):3897-3907
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1844618735036399616
score 13.070432