Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
- Autores
- Fraiman, D.; Pando, B.; Dargan, S.; Parker, I.; Dawson, S.P.
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
Fil:Dawson, S.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Biophys. J. 2006;90(11):3897-3907
- Materia
-
1,4,5 triphosphate
calcium
phosphate
unclassified drug
animal cell
article
controlled study
dynamics
endoplasmic reticulum
fluorescence
Kolmogorov Smirnov test
muscle cell
nonhuman
oocyte
Xenopus laevis
Neptunia - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00063495_v90_n11_p3897_Fraiman
Ver los metadatos del registro completo
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Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff intervalFraiman, D.Pando, B.Dargan, S.Parker, I.Dawson, S.P.1,4,5 triphosphatecalciumphosphateunclassified druganimal cellarticlecontrolled studydynamicsendoplasmic reticulumfluorescenceKolmogorov Smirnov testmuscle cellnonhumanoocyteXenopus laevisNeptuniaPuffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.Fil:Dawson, S.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00063495_v90_n11_p3897_FraimanBiophys. J. 2006;90(11):3897-3907reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:54Zpaperaa:paper_00063495_v90_n11_p3897_FraimanInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:56.035Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval |
title |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval |
spellingShingle |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval Fraiman, D. 1,4,5 triphosphate calcium phosphate unclassified drug animal cell article controlled study dynamics endoplasmic reticulum fluorescence Kolmogorov Smirnov test muscle cell nonhuman oocyte Xenopus laevis Neptunia |
title_short |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval |
title_full |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval |
title_fullStr |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval |
title_full_unstemmed |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval |
title_sort |
Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval |
dc.creator.none.fl_str_mv |
Fraiman, D. Pando, B. Dargan, S. Parker, I. Dawson, S.P. |
author |
Fraiman, D. |
author_facet |
Fraiman, D. Pando, B. Dargan, S. Parker, I. Dawson, S.P. |
author_role |
author |
author2 |
Pando, B. Dargan, S. Parker, I. Dawson, S.P. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
1,4,5 triphosphate calcium phosphate unclassified drug animal cell article controlled study dynamics endoplasmic reticulum fluorescence Kolmogorov Smirnov test muscle cell nonhuman oocyte Xenopus laevis Neptunia |
topic |
1,4,5 triphosphate calcium phosphate unclassified drug animal cell article controlled study dynamics endoplasmic reticulum fluorescence Kolmogorov Smirnov test muscle cell nonhuman oocyte Xenopus laevis Neptunia |
dc.description.none.fl_txt_mv |
Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society. Fil:Dawson, S.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00063495_v90_n11_p3897_Fraiman |
url |
http://hdl.handle.net/20.500.12110/paper_00063495_v90_n11_p3897_Fraiman |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Biophys. J. 2006;90(11):3897-3907 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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13.070432 |