Buffer regulation of calcium puff sequences

Autores
Fraiman Borrazás, Daniel Edmundo; Ponce Dawson, Silvina Martha
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Puffs are localized Ca2 + signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are the result of the liberation of Ca2 + from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. The presence of buffers that trap Ca2 + provides a mechanism that enriches the spatio–temporal dynamics of cytosolic calcium. The expression of different types of buffers along the cell's life provides a tool with which Ca2 + signals and their responses can be modulated. In this paper we extend the stochastic model of a cluster of IP3R-Ca2 + channels introduced previously to elucidate the effect of buffers on sequences of puffs at the same release site. We obtain analytically the probability laws of the interpuff time and of the number of channels that participate of the puffs. Furthermore, we show that under typical experimental conditions the effect of buffers can be accounted for in terms of a simple inhibiting function. Hence, by exploring different inhibiting functions we are able to study the effect of a variety of buffers on the puff size and interpuff time distributions. We find the somewhat counter-intuitive result that the addition of a fast Ca2 + buffer can increase the average number of channels that participate of a puff.
Fil: Fraiman Borrazás, Daniel Edmundo. Universidad de San Andres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
Calcium Dynamics
Puff Sequences
Buffers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17852
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Buffer regulation of calcium puff sequencesFraiman Borrazás, Daniel EdmundoPonce Dawson, Silvina MarthaCalcium DynamicsPuff SequencesBuffershttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Puffs are localized Ca2 + signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are the result of the liberation of Ca2 + from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. The presence of buffers that trap Ca2 + provides a mechanism that enriches the spatio–temporal dynamics of cytosolic calcium. The expression of different types of buffers along the cell's life provides a tool with which Ca2 + signals and their responses can be modulated. In this paper we extend the stochastic model of a cluster of IP3R-Ca2 + channels introduced previously to elucidate the effect of buffers on sequences of puffs at the same release site. We obtain analytically the probability laws of the interpuff time and of the number of channels that participate of the puffs. Furthermore, we show that under typical experimental conditions the effect of buffers can be accounted for in terms of a simple inhibiting function. Hence, by exploring different inhibiting functions we are able to study the effect of a variety of buffers on the puff size and interpuff time distributions. We find the somewhat counter-intuitive result that the addition of a fast Ca2 + buffer can increase the average number of channels that participate of a puff.Fil: Fraiman Borrazás, Daniel Edmundo. Universidad de San Andres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaIop Publishing2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17852Fraiman Borrazás, Daniel Edmundo; Ponce Dawson, Silvina Martha; Buffer regulation of calcium puff sequences; Iop Publishing; Physical Biology; 11; 1; 1-2014; 1-11; 0160071478-3967enginfo:eu-repo/semantics/altIdentifier/doi/10.1088/1478-3975/11/1/016007info:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1478-3975/11/1/016007/metainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:22Zoai:ri.conicet.gov.ar:11336/17852instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:22.99CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Buffer regulation of calcium puff sequences
title Buffer regulation of calcium puff sequences
spellingShingle Buffer regulation of calcium puff sequences
Fraiman Borrazás, Daniel Edmundo
Calcium Dynamics
Puff Sequences
Buffers
title_short Buffer regulation of calcium puff sequences
title_full Buffer regulation of calcium puff sequences
title_fullStr Buffer regulation of calcium puff sequences
title_full_unstemmed Buffer regulation of calcium puff sequences
title_sort Buffer regulation of calcium puff sequences
dc.creator.none.fl_str_mv Fraiman Borrazás, Daniel Edmundo
Ponce Dawson, Silvina Martha
author Fraiman Borrazás, Daniel Edmundo
author_facet Fraiman Borrazás, Daniel Edmundo
Ponce Dawson, Silvina Martha
author_role author
author2 Ponce Dawson, Silvina Martha
author2_role author
dc.subject.none.fl_str_mv Calcium Dynamics
Puff Sequences
Buffers
topic Calcium Dynamics
Puff Sequences
Buffers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Puffs are localized Ca2 + signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are the result of the liberation of Ca2 + from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. The presence of buffers that trap Ca2 + provides a mechanism that enriches the spatio–temporal dynamics of cytosolic calcium. The expression of different types of buffers along the cell's life provides a tool with which Ca2 + signals and their responses can be modulated. In this paper we extend the stochastic model of a cluster of IP3R-Ca2 + channels introduced previously to elucidate the effect of buffers on sequences of puffs at the same release site. We obtain analytically the probability laws of the interpuff time and of the number of channels that participate of the puffs. Furthermore, we show that under typical experimental conditions the effect of buffers can be accounted for in terms of a simple inhibiting function. Hence, by exploring different inhibiting functions we are able to study the effect of a variety of buffers on the puff size and interpuff time distributions. We find the somewhat counter-intuitive result that the addition of a fast Ca2 + buffer can increase the average number of channels that participate of a puff.
Fil: Fraiman Borrazás, Daniel Edmundo. Universidad de San Andres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description Puffs are localized Ca2 + signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are the result of the liberation of Ca2 + from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. The presence of buffers that trap Ca2 + provides a mechanism that enriches the spatio–temporal dynamics of cytosolic calcium. The expression of different types of buffers along the cell's life provides a tool with which Ca2 + signals and their responses can be modulated. In this paper we extend the stochastic model of a cluster of IP3R-Ca2 + channels introduced previously to elucidate the effect of buffers on sequences of puffs at the same release site. We obtain analytically the probability laws of the interpuff time and of the number of channels that participate of the puffs. Furthermore, we show that under typical experimental conditions the effect of buffers can be accounted for in terms of a simple inhibiting function. Hence, by exploring different inhibiting functions we are able to study the effect of a variety of buffers on the puff size and interpuff time distributions. We find the somewhat counter-intuitive result that the addition of a fast Ca2 + buffer can increase the average number of channels that participate of a puff.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17852
Fraiman Borrazás, Daniel Edmundo; Ponce Dawson, Silvina Martha; Buffer regulation of calcium puff sequences; Iop Publishing; Physical Biology; 11; 1; 1-2014; 1-11; 016007
1478-3967
url http://hdl.handle.net/11336/17852
identifier_str_mv Fraiman Borrazás, Daniel Edmundo; Ponce Dawson, Silvina Martha; Buffer regulation of calcium puff sequences; Iop Publishing; Physical Biology; 11; 1; 1-2014; 1-11; 016007
1478-3967
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1088/1478-3975/11/1/016007
info:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1478-3975/11/1/016007/meta
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Iop Publishing
publisher.none.fl_str_mv Iop Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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