Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results
- Autores
- Guevara, M. A.; Vaquero, A.; Fernandez Diez, Marina; Márquez Herrero, S.; García Menéndez, Sebastián Marcelo Manuel; Romanowic, E.; Barrutieta, I.; Bengoetxea Odriozola, H.; Ortuzar Markes, N.; Baiardi, G.; Lafuente Sanchez, J. V.; Gargiulo, Pascual Angel
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- There are different pharmacological treatments for depression, limited in their effectiveness and many have very long latency periods. Current research points to NMDA antagonists as possible therapeutic targets of this disorder. The objective of this work was to assess at a behavioral level the synergistic interactions between the antidepressant of the SSRIs group fluoxetine, and ketamine, a noncompetitive NMDA receptor antagonist and correlate the findings with structural changes in the basolateral nucleus of the tonsil with respect to positive calbindin Interneurons. Holtzman rats that were treated with fluoxetine were used. 10, 15 and 20 mg / kg and ketamine. 2.5, 5 and 10 mg / kg and subsequently were evaluated in the forced swim test each group consisted of n = 20. Fluoxetine in doses of 10 and 15 mg / kg and ketamine in doses of 2.5 and 5 mg / kg did not produce a significant decrease in immobility time, On the contrary, if significant decreases were observed with fluoxetine 20 mg / kg (p <0.01) and ketamine 10 mg / kg (p <0.05). Likewise, there was a significant decrease in immobility time when fluoxetine 10 mg / kg + ketamine 2.5 mg / kg (p <0.01) and fluoxetine 15 mg / kg + ketamine 5 mg / kg (p <0,0001). At 24 hours after the test, positive calbindin interneurons of the basolateral nucleus of the tonsil were analyzed by immunohistochemistry. Each group consisted of an n = 3. It was observed that the saline group presented significant differences with the group treated with 20 mg / kg of fluoxetine (p = 0.001), with the group treated with 5 mg / kg of fluoxetine (p = 0.0001) and with the group treated with 5 mg / kg of fluoxetine + 2.5 mg / kg of ketamine (p = 0.0001), the latter being the one with the least positive calbindin interneurons. Ketamine groups have not yet been analyzed. These results indicate that co-administration of fluoxetine and ketamine can induce a more potent antidepressant activity than when used alone. In addition, the decrease in the density of cositive calbindin interneurons in the basolateral nucleus of the amygdala could be directly related to the action of drugs on these neuronal populations, thereby increasing neuronal plasticity and being able to restore excitatory and inhibitory balance.
Fil: Guevara, M. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Vaquero, A.. Universidad del País Vasco; España
Fil: Fernandez Diez, Marina. Universidad del País Vasco; España
Fil: Márquez Herrero, S.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: García Menéndez, Sebastián Marcelo Manuel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romanowic, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Barrutieta, I.. Universidad del País Vasco; España
Fil: Bengoetxea Odriozola, H.. Universidad del País Vasco; España
Fil: Ortuzar Markes, N.. Universidad del País Vasco; España
Fil: Baiardi, G.. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Lafuente Sanchez, J. V.. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Gargiulo, Pascual Angel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo - Materia
-
KETAMINE
FLUOXETINE
JOINT
ADMINISTRATION
CALBINDINE
INTERNEURONS
BASOLATERAL
AMYGDALA
RAT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/140013
Ver los metadatos del registro completo
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Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary resultsGuevara, M. A.Vaquero, A.Fernandez Diez, MarinaMárquez Herrero, S.García Menéndez, Sebastián Marcelo ManuelRomanowic, E.Barrutieta, I.Bengoetxea Odriozola, H.Ortuzar Markes, N.Baiardi, G.Lafuente Sanchez, J. V.Gargiulo, Pascual AngelKETAMINEFLUOXETINEJOINTADMINISTRATIONCALBINDINEINTERNEURONSBASOLATERALAMYGDALARAThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3There are different pharmacological treatments for depression, limited in their effectiveness and many have very long latency periods. Current research points to NMDA antagonists as possible therapeutic targets of this disorder. The objective of this work was to assess at a behavioral level the synergistic interactions between the antidepressant of the SSRIs group fluoxetine, and ketamine, a noncompetitive NMDA receptor antagonist and correlate the findings with structural changes in the basolateral nucleus of the tonsil with respect to positive calbindin Interneurons. Holtzman rats that were treated with fluoxetine were used. 10, 15 and 20 mg / kg and ketamine. 2.5, 5 and 10 mg / kg and subsequently were evaluated in the forced swim test each group consisted of n = 20. Fluoxetine in doses of 10 and 15 mg / kg and ketamine in doses of 2.5 and 5 mg / kg did not produce a significant decrease in immobility time, On the contrary, if significant decreases were observed with fluoxetine 20 mg / kg (p <0.01) and ketamine 10 mg / kg (p <0.05). Likewise, there was a significant decrease in immobility time when fluoxetine 10 mg / kg + ketamine 2.5 mg / kg (p <0.01) and fluoxetine 15 mg / kg + ketamine 5 mg / kg (p <0,0001). At 24 hours after the test, positive calbindin interneurons of the basolateral nucleus of the tonsil were analyzed by immunohistochemistry. Each group consisted of an n = 3. It was observed that the saline group presented significant differences with the group treated with 20 mg / kg of fluoxetine (p = 0.001), with the group treated with 5 mg / kg of fluoxetine (p = 0.0001) and with the group treated with 5 mg / kg of fluoxetine + 2.5 mg / kg of ketamine (p = 0.0001), the latter being the one with the least positive calbindin interneurons. Ketamine groups have not yet been analyzed. These results indicate that co-administration of fluoxetine and ketamine can induce a more potent antidepressant activity than when used alone. In addition, the decrease in the density of cositive calbindin interneurons in the basolateral nucleus of the amygdala could be directly related to the action of drugs on these neuronal populations, thereby increasing neuronal plasticity and being able to restore excitatory and inhibitory balance.Fil: Guevara, M. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Vaquero, A.. Universidad del País Vasco; EspañaFil: Fernandez Diez, Marina. Universidad del País Vasco; EspañaFil: Márquez Herrero, S.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: García Menéndez, Sebastián Marcelo Manuel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romanowic, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Barrutieta, I.. Universidad del País Vasco; EspañaFil: Bengoetxea Odriozola, H.. Universidad del País Vasco; EspañaFil: Ortuzar Markes, N.. Universidad del País Vasco; EspañaFil: Baiardi, G.. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Lafuente Sanchez, J. V.. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Gargiulo, Pascual Angel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140013Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 45-45CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:36Zoai:ri.conicet.gov.ar:11336/140013instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:37.144CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results |
| title |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results |
| spellingShingle |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results Guevara, M. A. KETAMINE FLUOXETINE JOINT ADMINISTRATION CALBINDINE INTERNEURONS BASOLATERAL AMYGDALA RAT |
| title_short |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results |
| title_full |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results |
| title_fullStr |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results |
| title_full_unstemmed |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results |
| title_sort |
Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results |
| dc.creator.none.fl_str_mv |
Guevara, M. A. Vaquero, A. Fernandez Diez, Marina Márquez Herrero, S. García Menéndez, Sebastián Marcelo Manuel Romanowic, E. Barrutieta, I. Bengoetxea Odriozola, H. Ortuzar Markes, N. Baiardi, G. Lafuente Sanchez, J. V. Gargiulo, Pascual Angel |
| author |
Guevara, M. A. |
| author_facet |
Guevara, M. A. Vaquero, A. Fernandez Diez, Marina Márquez Herrero, S. García Menéndez, Sebastián Marcelo Manuel Romanowic, E. Barrutieta, I. Bengoetxea Odriozola, H. Ortuzar Markes, N. Baiardi, G. Lafuente Sanchez, J. V. Gargiulo, Pascual Angel |
| author_role |
author |
| author2 |
Vaquero, A. Fernandez Diez, Marina Márquez Herrero, S. García Menéndez, Sebastián Marcelo Manuel Romanowic, E. Barrutieta, I. Bengoetxea Odriozola, H. Ortuzar Markes, N. Baiardi, G. Lafuente Sanchez, J. V. Gargiulo, Pascual Angel |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
KETAMINE FLUOXETINE JOINT ADMINISTRATION CALBINDINE INTERNEURONS BASOLATERAL AMYGDALA RAT |
| topic |
KETAMINE FLUOXETINE JOINT ADMINISTRATION CALBINDINE INTERNEURONS BASOLATERAL AMYGDALA RAT |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
There are different pharmacological treatments for depression, limited in their effectiveness and many have very long latency periods. Current research points to NMDA antagonists as possible therapeutic targets of this disorder. The objective of this work was to assess at a behavioral level the synergistic interactions between the antidepressant of the SSRIs group fluoxetine, and ketamine, a noncompetitive NMDA receptor antagonist and correlate the findings with structural changes in the basolateral nucleus of the tonsil with respect to positive calbindin Interneurons. Holtzman rats that were treated with fluoxetine were used. 10, 15 and 20 mg / kg and ketamine. 2.5, 5 and 10 mg / kg and subsequently were evaluated in the forced swim test each group consisted of n = 20. Fluoxetine in doses of 10 and 15 mg / kg and ketamine in doses of 2.5 and 5 mg / kg did not produce a significant decrease in immobility time, On the contrary, if significant decreases were observed with fluoxetine 20 mg / kg (p <0.01) and ketamine 10 mg / kg (p <0.05). Likewise, there was a significant decrease in immobility time when fluoxetine 10 mg / kg + ketamine 2.5 mg / kg (p <0.01) and fluoxetine 15 mg / kg + ketamine 5 mg / kg (p <0,0001). At 24 hours after the test, positive calbindin interneurons of the basolateral nucleus of the tonsil were analyzed by immunohistochemistry. Each group consisted of an n = 3. It was observed that the saline group presented significant differences with the group treated with 20 mg / kg of fluoxetine (p = 0.001), with the group treated with 5 mg / kg of fluoxetine (p = 0.0001) and with the group treated with 5 mg / kg of fluoxetine + 2.5 mg / kg of ketamine (p = 0.0001), the latter being the one with the least positive calbindin interneurons. Ketamine groups have not yet been analyzed. These results indicate that co-administration of fluoxetine and ketamine can induce a more potent antidepressant activity than when used alone. In addition, the decrease in the density of cositive calbindin interneurons in the basolateral nucleus of the amygdala could be directly related to the action of drugs on these neuronal populations, thereby increasing neuronal plasticity and being able to restore excitatory and inhibitory balance. Fil: Guevara, M. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Vaquero, A.. Universidad del País Vasco; España Fil: Fernandez Diez, Marina. Universidad del País Vasco; España Fil: Márquez Herrero, S.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: García Menéndez, Sebastián Marcelo Manuel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romanowic, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Barrutieta, I.. Universidad del País Vasco; España Fil: Bengoetxea Odriozola, H.. Universidad del País Vasco; España Fil: Ortuzar Markes, N.. Universidad del País Vasco; España Fil: Baiardi, G.. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Lafuente Sanchez, J. V.. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Gargiulo, Pascual Angel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo San Luis Argentina Sociedad de Biología de Cuyo |
| description |
There are different pharmacological treatments for depression, limited in their effectiveness and many have very long latency periods. Current research points to NMDA antagonists as possible therapeutic targets of this disorder. The objective of this work was to assess at a behavioral level the synergistic interactions between the antidepressant of the SSRIs group fluoxetine, and ketamine, a noncompetitive NMDA receptor antagonist and correlate the findings with structural changes in the basolateral nucleus of the tonsil with respect to positive calbindin Interneurons. Holtzman rats that were treated with fluoxetine were used. 10, 15 and 20 mg / kg and ketamine. 2.5, 5 and 10 mg / kg and subsequently were evaluated in the forced swim test each group consisted of n = 20. Fluoxetine in doses of 10 and 15 mg / kg and ketamine in doses of 2.5 and 5 mg / kg did not produce a significant decrease in immobility time, On the contrary, if significant decreases were observed with fluoxetine 20 mg / kg (p <0.01) and ketamine 10 mg / kg (p <0.05). Likewise, there was a significant decrease in immobility time when fluoxetine 10 mg / kg + ketamine 2.5 mg / kg (p <0.01) and fluoxetine 15 mg / kg + ketamine 5 mg / kg (p <0,0001). At 24 hours after the test, positive calbindin interneurons of the basolateral nucleus of the tonsil were analyzed by immunohistochemistry. Each group consisted of an n = 3. It was observed that the saline group presented significant differences with the group treated with 20 mg / kg of fluoxetine (p = 0.001), with the group treated with 5 mg / kg of fluoxetine (p = 0.0001) and with the group treated with 5 mg / kg of fluoxetine + 2.5 mg / kg of ketamine (p = 0.0001), the latter being the one with the least positive calbindin interneurons. Ketamine groups have not yet been analyzed. These results indicate that co-administration of fluoxetine and ketamine can induce a more potent antidepressant activity than when used alone. In addition, the decrease in the density of cositive calbindin interneurons in the basolateral nucleus of the amygdala could be directly related to the action of drugs on these neuronal populations, thereby increasing neuronal plasticity and being able to restore excitatory and inhibitory balance. |
| publishDate |
2019 |
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2019 |
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http://hdl.handle.net/11336/140013 Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 45-45 CONICET Digital CONICET |
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http://hdl.handle.net/11336/140013 |
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Effect of the joint administration of ketamine and fluoxetine in positive calbindine interneurons of the basilateral nucleus of amígdala in ratus novergicus: preliminary results; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 45-45 CONICET Digital CONICET |
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eng |
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eng |
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Sociedad de Biología de Cuyo |
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Sociedad de Biología de Cuyo |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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