Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis

Autores
Wu, Hongyan; Gao, Kunshan; Villafañe, Virginia Estela; Watanabe, Teruo; Helbling, Eduardo Walter
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which had been grown under sunlight for decades) while they were cultured with three solar radiation treatments: PAB (photosynthetically active radiation [PAR] plus UVR; 280 to 700 nm), PA (PAR plus UV-A; 320 to 700 nm), and P (PAR only; 400 to 700 nm). Solar UVR broke the spiral filaments of A. platensis exposed to full solar radiation in short-term low-cell-density cultures. This breakage was observed after 2 h for the indoor strain but after 4 to 6 h for the outdoor strain. Filament breakage also occurred in the cultures exposed to PAR alone; however, the extent of breakage was less than that observed for filaments exposed to full solar radiation. The spiral filaments broke and compressed when high-cell-density cultures were exposed to full solar radiation during long-term experiments. When UV-B was screened off, the filaments initially broke, but they elongated and became loosely arranged later (i.e., there were fewer spirals per unit of filament length). When UVR was filtered out, the spiral structure hardly broke or became looser. Photosynthetic O2 evolution in the presence of UVR was significantly suppressed in the indoor strain compared to the outdoor strain. UVRinduced inhibition increased with exposure time, and it was significantly lower in the outdoor strain. The concentration of UV-absorbing compounds was low in both strains, and there was no significant change in the amount regardless of the radiation treatment, suggesting that these compounds were not effectively used as protection against solar UVR. Self-shading, on the other hand, produced by compression of the spirals over adaptive time scales, seems to play an important role in protecting this species against deleterious UVR. Our findings suggest that the increase in UV-B irradiance due to ozone depletion not only might affect photosynthesis but also might alter the morphological development of filamentous cyanobacteria during acclimation or over adaptive time scales.
Fil: Wu, Hongyan. Shantou University; China
Fil: Gao, Kunshan. Chinese Academy of Sciences; República de China. Shantou University; China
Fil: Villafañe, Virginia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Fil: Watanabe, Teruo. Hainan DIC Microalgae Co.; China
Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Materia
Solar UV Radiation
Morphology
Photosynthesis
Cyanobacterium Arthrospira platensis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105259

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spelling Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensisWu, HongyanGao, KunshanVillafañe, Virginia EstelaWatanabe, TeruoHelbling, Eduardo WalterSolar UV RadiationMorphologyPhotosynthesisCyanobacterium Arthrospira platensishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which had been grown under sunlight for decades) while they were cultured with three solar radiation treatments: PAB (photosynthetically active radiation [PAR] plus UVR; 280 to 700 nm), PA (PAR plus UV-A; 320 to 700 nm), and P (PAR only; 400 to 700 nm). Solar UVR broke the spiral filaments of A. platensis exposed to full solar radiation in short-term low-cell-density cultures. This breakage was observed after 2 h for the indoor strain but after 4 to 6 h for the outdoor strain. Filament breakage also occurred in the cultures exposed to PAR alone; however, the extent of breakage was less than that observed for filaments exposed to full solar radiation. The spiral filaments broke and compressed when high-cell-density cultures were exposed to full solar radiation during long-term experiments. When UV-B was screened off, the filaments initially broke, but they elongated and became loosely arranged later (i.e., there were fewer spirals per unit of filament length). When UVR was filtered out, the spiral structure hardly broke or became looser. Photosynthetic O2 evolution in the presence of UVR was significantly suppressed in the indoor strain compared to the outdoor strain. UVRinduced inhibition increased with exposure time, and it was significantly lower in the outdoor strain. The concentration of UV-absorbing compounds was low in both strains, and there was no significant change in the amount regardless of the radiation treatment, suggesting that these compounds were not effectively used as protection against solar UVR. Self-shading, on the other hand, produced by compression of the spirals over adaptive time scales, seems to play an important role in protecting this species against deleterious UVR. Our findings suggest that the increase in UV-B irradiance due to ozone depletion not only might affect photosynthesis but also might alter the morphological development of filamentous cyanobacteria during acclimation or over adaptive time scales.Fil: Wu, Hongyan. Shantou University; ChinaFil: Gao, Kunshan. Chinese Academy of Sciences; República de China. Shantou University; ChinaFil: Villafañe, Virginia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; ArgentinaFil: Watanabe, Teruo. Hainan DIC Microalgae Co.; ChinaFil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; ArgentinaAmerican Society for Microbiology2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105259Wu, Hongyan; Gao, Kunshan; Villafañe, Virginia Estela; Watanabe, Teruo; Helbling, Eduardo Walter; Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis; American Society for Microbiology; Applied And Environmental Microbiology; 71; 9; 9-2005; 5004-50130099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.71.9.5004-5013.2005info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/71/9info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/71/9/5004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:33Zoai:ri.conicet.gov.ar:11336/105259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:34.279CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
title Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
spellingShingle Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
Wu, Hongyan
Solar UV Radiation
Morphology
Photosynthesis
Cyanobacterium Arthrospira platensis
title_short Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
title_full Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
title_fullStr Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
title_full_unstemmed Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
title_sort Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis
dc.creator.none.fl_str_mv Wu, Hongyan
Gao, Kunshan
Villafañe, Virginia Estela
Watanabe, Teruo
Helbling, Eduardo Walter
author Wu, Hongyan
author_facet Wu, Hongyan
Gao, Kunshan
Villafañe, Virginia Estela
Watanabe, Teruo
Helbling, Eduardo Walter
author_role author
author2 Gao, Kunshan
Villafañe, Virginia Estela
Watanabe, Teruo
Helbling, Eduardo Walter
author2_role author
author
author
author
dc.subject.none.fl_str_mv Solar UV Radiation
Morphology
Photosynthesis
Cyanobacterium Arthrospira platensis
topic Solar UV Radiation
Morphology
Photosynthesis
Cyanobacterium Arthrospira platensis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which had been grown under sunlight for decades) while they were cultured with three solar radiation treatments: PAB (photosynthetically active radiation [PAR] plus UVR; 280 to 700 nm), PA (PAR plus UV-A; 320 to 700 nm), and P (PAR only; 400 to 700 nm). Solar UVR broke the spiral filaments of A. platensis exposed to full solar radiation in short-term low-cell-density cultures. This breakage was observed after 2 h for the indoor strain but after 4 to 6 h for the outdoor strain. Filament breakage also occurred in the cultures exposed to PAR alone; however, the extent of breakage was less than that observed for filaments exposed to full solar radiation. The spiral filaments broke and compressed when high-cell-density cultures were exposed to full solar radiation during long-term experiments. When UV-B was screened off, the filaments initially broke, but they elongated and became loosely arranged later (i.e., there were fewer spirals per unit of filament length). When UVR was filtered out, the spiral structure hardly broke or became looser. Photosynthetic O2 evolution in the presence of UVR was significantly suppressed in the indoor strain compared to the outdoor strain. UVRinduced inhibition increased with exposure time, and it was significantly lower in the outdoor strain. The concentration of UV-absorbing compounds was low in both strains, and there was no significant change in the amount regardless of the radiation treatment, suggesting that these compounds were not effectively used as protection against solar UVR. Self-shading, on the other hand, produced by compression of the spirals over adaptive time scales, seems to play an important role in protecting this species against deleterious UVR. Our findings suggest that the increase in UV-B irradiance due to ozone depletion not only might affect photosynthesis but also might alter the morphological development of filamentous cyanobacteria during acclimation or over adaptive time scales.
Fil: Wu, Hongyan. Shantou University; China
Fil: Gao, Kunshan. Chinese Academy of Sciences; República de China. Shantou University; China
Fil: Villafañe, Virginia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Fil: Watanabe, Teruo. Hainan DIC Microalgae Co.; China
Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
description To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which had been grown under sunlight for decades) while they were cultured with three solar radiation treatments: PAB (photosynthetically active radiation [PAR] plus UVR; 280 to 700 nm), PA (PAR plus UV-A; 320 to 700 nm), and P (PAR only; 400 to 700 nm). Solar UVR broke the spiral filaments of A. platensis exposed to full solar radiation in short-term low-cell-density cultures. This breakage was observed after 2 h for the indoor strain but after 4 to 6 h for the outdoor strain. Filament breakage also occurred in the cultures exposed to PAR alone; however, the extent of breakage was less than that observed for filaments exposed to full solar radiation. The spiral filaments broke and compressed when high-cell-density cultures were exposed to full solar radiation during long-term experiments. When UV-B was screened off, the filaments initially broke, but they elongated and became loosely arranged later (i.e., there were fewer spirals per unit of filament length). When UVR was filtered out, the spiral structure hardly broke or became looser. Photosynthetic O2 evolution in the presence of UVR was significantly suppressed in the indoor strain compared to the outdoor strain. UVRinduced inhibition increased with exposure time, and it was significantly lower in the outdoor strain. The concentration of UV-absorbing compounds was low in both strains, and there was no significant change in the amount regardless of the radiation treatment, suggesting that these compounds were not effectively used as protection against solar UVR. Self-shading, on the other hand, produced by compression of the spirals over adaptive time scales, seems to play an important role in protecting this species against deleterious UVR. Our findings suggest that the increase in UV-B irradiance due to ozone depletion not only might affect photosynthesis but also might alter the morphological development of filamentous cyanobacteria during acclimation or over adaptive time scales.
publishDate 2005
dc.date.none.fl_str_mv 2005-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105259
Wu, Hongyan; Gao, Kunshan; Villafañe, Virginia Estela; Watanabe, Teruo; Helbling, Eduardo Walter; Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis; American Society for Microbiology; Applied And Environmental Microbiology; 71; 9; 9-2005; 5004-5013
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105259
identifier_str_mv Wu, Hongyan; Gao, Kunshan; Villafañe, Virginia Estela; Watanabe, Teruo; Helbling, Eduardo Walter; Effects of Solar UV Radiation on Morphology and Photosynthesis of Filamentous Cyanobacterium Arthrospira platensis; American Society for Microbiology; Applied And Environmental Microbiology; 71; 9; 9-2005; 5004-5013
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.71.9.5004-5013.2005
info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/71/9
info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/71/9/5004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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