Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration

Autores
Taranto, Maria Pia; Perez Martinez, Gaspar; Font, Graciela Maria
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactic acid bacteria and other species dwelling in the gut must be tolerant to bile salts. This study sought to determine the effects of the bile salts taurodeoxycholate (TDCA) and deoxycholate (DCA) on Lactobacillus reuteri CRL 1098, a strain of likely probiotic value. When compared to other lactobacilli, L. reuteri showed the highest survival rate but remained sensitive to high (>3 mM) DCA concentrations. DCA produced complete permeabilization of cells, abolished glucose uptake and severely distorted the cell envelope, as shown by electron microscopy. Detailed analytical studies revealed a change in the phospholipid to glycolipid ratio, and also in lipid proportions. The C18:1 W9C form remarkably increased, possibly following a rapid adaptive response during DCA treatment. This study provides the first solid evidence of the mechanism of DCA toxicity in a lactic acid bacterium.
Fil: Taranto, Maria Pia. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Perez Martinez, Gaspar. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Materia
Cell Membrane
Deoxycholic Acid
Lactic Acid Bacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36579
Acceder
id CONICETDig_14f13d8ec780166f5638514252f4cb33
oai_identifier_str oai:ri.conicet.gov.ar:11336/36579
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administrationTaranto, Maria PiaPerez Martinez, GasparFont, Graciela MariaCell MembraneDeoxycholic AcidLactic Acid Bacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactic acid bacteria and other species dwelling in the gut must be tolerant to bile salts. This study sought to determine the effects of the bile salts taurodeoxycholate (TDCA) and deoxycholate (DCA) on Lactobacillus reuteri CRL 1098, a strain of likely probiotic value. When compared to other lactobacilli, L. reuteri showed the highest survival rate but remained sensitive to high (>3 mM) DCA concentrations. DCA produced complete permeabilization of cells, abolished glucose uptake and severely distorted the cell envelope, as shown by electron microscopy. Detailed analytical studies revealed a change in the phospholipid to glycolipid ratio, and also in lipid proportions. The C18:1 W9C form remarkably increased, possibly following a rapid adaptive response during DCA treatment. This study provides the first solid evidence of the mechanism of DCA toxicity in a lactic acid bacterium.Fil: Taranto, Maria Pia. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Perez Martinez, Gaspar. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaElsevier Science2006-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36579Taranto, Maria Pia; Perez Martinez, Gaspar; Font, Graciela Maria; Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration; Elsevier Science; Research In Microbiology; 157; 8; 10-2006; 720-7250923-2508CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2006.04.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0923250806001021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:47:52Zoai:ri.conicet.gov.ar:11336/36579instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:47:53.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
title Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
spellingShingle Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
Taranto, Maria Pia
Cell Membrane
Deoxycholic Acid
Lactic Acid Bacteria
title_short Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
title_full Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
title_fullStr Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
title_full_unstemmed Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
title_sort Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration
dc.creator.none.fl_str_mv Taranto, Maria Pia
Perez Martinez, Gaspar
Font, Graciela Maria
author Taranto, Maria Pia
author_facet Taranto, Maria Pia
Perez Martinez, Gaspar
Font, Graciela Maria
author_role author
author2 Perez Martinez, Gaspar
Font, Graciela Maria
author2_role author
author
dc.subject.none.fl_str_mv Cell Membrane
Deoxycholic Acid
Lactic Acid Bacteria
topic Cell Membrane
Deoxycholic Acid
Lactic Acid Bacteria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactic acid bacteria and other species dwelling in the gut must be tolerant to bile salts. This study sought to determine the effects of the bile salts taurodeoxycholate (TDCA) and deoxycholate (DCA) on Lactobacillus reuteri CRL 1098, a strain of likely probiotic value. When compared to other lactobacilli, L. reuteri showed the highest survival rate but remained sensitive to high (>3 mM) DCA concentrations. DCA produced complete permeabilization of cells, abolished glucose uptake and severely distorted the cell envelope, as shown by electron microscopy. Detailed analytical studies revealed a change in the phospholipid to glycolipid ratio, and also in lipid proportions. The C18:1 W9C form remarkably increased, possibly following a rapid adaptive response during DCA treatment. This study provides the first solid evidence of the mechanism of DCA toxicity in a lactic acid bacterium.
Fil: Taranto, Maria Pia. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Perez Martinez, Gaspar. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
description Lactic acid bacteria and other species dwelling in the gut must be tolerant to bile salts. This study sought to determine the effects of the bile salts taurodeoxycholate (TDCA) and deoxycholate (DCA) on Lactobacillus reuteri CRL 1098, a strain of likely probiotic value. When compared to other lactobacilli, L. reuteri showed the highest survival rate but remained sensitive to high (>3 mM) DCA concentrations. DCA produced complete permeabilization of cells, abolished glucose uptake and severely distorted the cell envelope, as shown by electron microscopy. Detailed analytical studies revealed a change in the phospholipid to glycolipid ratio, and also in lipid proportions. The C18:1 W9C form remarkably increased, possibly following a rapid adaptive response during DCA treatment. This study provides the first solid evidence of the mechanism of DCA toxicity in a lactic acid bacterium.
publishDate 2006
dc.date.none.fl_str_mv 2006-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36579
Taranto, Maria Pia; Perez Martinez, Gaspar; Font, Graciela Maria; Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration; Elsevier Science; Research In Microbiology; 157; 8; 10-2006; 720-725
0923-2508
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36579
identifier_str_mv Taranto, Maria Pia; Perez Martinez, Gaspar; Font, Graciela Maria; Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration; Elsevier Science; Research In Microbiology; 157; 8; 10-2006; 720-725
0923-2508
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2006.04.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0923250806001021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782184304672768
score 12.982451

Publicaciones similares