Single-chain antibody fragments: Purification methodologies

Autores
Pellegrini Malpiedi, Luciana; Diaz, César A.; Nerli, Bibiana Beatriz; Pessoa, Adalberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
At present, single-chain variable fragments (scFv) of antibodies are considered one of the most important tools in human therapies. Wide applications of antibodies are being exploited in different medical, pharmaceutical and research areas. These molecules maintain the same binding functionality that full length antibodies but possess several advantageous features as quickness to penetrate the tissues, easy manipulation, fast elimination of their immunocomplex and the possibility of being produced in simple expression systems like bacteria and yeast. The increasing demand in antibody based methodologies is driving advances in the production and purification of genetically engineered antibodies and antibody fragments. While advances in expression systems allow the production of high titers of antibodies, there exist some limits imposed by the downstream methodologies which are not efficient enough to ensure their industrialization. The main aim of this review is to highlight the principal characteristics of single-chain variable fragments of antibodies addressing advances and perspectives on scFv purification.
Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz, César A.. Universidade de Sao Paulo; Brasil
Fil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pessoa, Adalberto. Universidade de Sao Paulo; Brasil
Materia
Immunoglobulin
Single-Chain Variable Fragment
First Capture
Platform Methodology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21592
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Single-chain antibody fragments: Purification methodologiesPellegrini Malpiedi, LucianaDiaz, César A.Nerli, Bibiana BeatrizPessoa, AdalbertoImmunoglobulinSingle-Chain Variable FragmentFirst CapturePlatform Methodologyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1At present, single-chain variable fragments (scFv) of antibodies are considered one of the most important tools in human therapies. Wide applications of antibodies are being exploited in different medical, pharmaceutical and research areas. These molecules maintain the same binding functionality that full length antibodies but possess several advantageous features as quickness to penetrate the tissues, easy manipulation, fast elimination of their immunocomplex and the possibility of being produced in simple expression systems like bacteria and yeast. The increasing demand in antibody based methodologies is driving advances in the production and purification of genetically engineered antibodies and antibody fragments. While advances in expression systems allow the production of high titers of antibodies, there exist some limits imposed by the downstream methodologies which are not efficient enough to ensure their industrialization. The main aim of this review is to highlight the principal characteristics of single-chain variable fragments of antibodies addressing advances and perspectives on scFv purification.Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diaz, César A.. Universidade de Sao Paulo; BrasilFil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pessoa, Adalberto. Universidade de Sao Paulo; BrasilElsevier2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21592Pellegrini Malpiedi, Luciana; Diaz, César A.; Nerli, Bibiana Beatriz; Pessoa, Adalberto; Single-chain antibody fragments: Purification methodologies; Elsevier; Process Biochemistry; 48; 8; 6-2013; 1242-12511359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2013.06.008info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S135951131300278Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:32Zoai:ri.conicet.gov.ar:11336/21592instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:33.097CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Single-chain antibody fragments: Purification methodologies
title Single-chain antibody fragments: Purification methodologies
spellingShingle Single-chain antibody fragments: Purification methodologies
Pellegrini Malpiedi, Luciana
Immunoglobulin
Single-Chain Variable Fragment
First Capture
Platform Methodology
title_short Single-chain antibody fragments: Purification methodologies
title_full Single-chain antibody fragments: Purification methodologies
title_fullStr Single-chain antibody fragments: Purification methodologies
title_full_unstemmed Single-chain antibody fragments: Purification methodologies
title_sort Single-chain antibody fragments: Purification methodologies
dc.creator.none.fl_str_mv Pellegrini Malpiedi, Luciana
Diaz, César A.
Nerli, Bibiana Beatriz
Pessoa, Adalberto
author Pellegrini Malpiedi, Luciana
author_facet Pellegrini Malpiedi, Luciana
Diaz, César A.
Nerli, Bibiana Beatriz
Pessoa, Adalberto
author_role author
author2 Diaz, César A.
Nerli, Bibiana Beatriz
Pessoa, Adalberto
author2_role author
author
author
dc.subject.none.fl_str_mv Immunoglobulin
Single-Chain Variable Fragment
First Capture
Platform Methodology
topic Immunoglobulin
Single-Chain Variable Fragment
First Capture
Platform Methodology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv At present, single-chain variable fragments (scFv) of antibodies are considered one of the most important tools in human therapies. Wide applications of antibodies are being exploited in different medical, pharmaceutical and research areas. These molecules maintain the same binding functionality that full length antibodies but possess several advantageous features as quickness to penetrate the tissues, easy manipulation, fast elimination of their immunocomplex and the possibility of being produced in simple expression systems like bacteria and yeast. The increasing demand in antibody based methodologies is driving advances in the production and purification of genetically engineered antibodies and antibody fragments. While advances in expression systems allow the production of high titers of antibodies, there exist some limits imposed by the downstream methodologies which are not efficient enough to ensure their industrialization. The main aim of this review is to highlight the principal characteristics of single-chain variable fragments of antibodies addressing advances and perspectives on scFv purification.
Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz, César A.. Universidade de Sao Paulo; Brasil
Fil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pessoa, Adalberto. Universidade de Sao Paulo; Brasil
description At present, single-chain variable fragments (scFv) of antibodies are considered one of the most important tools in human therapies. Wide applications of antibodies are being exploited in different medical, pharmaceutical and research areas. These molecules maintain the same binding functionality that full length antibodies but possess several advantageous features as quickness to penetrate the tissues, easy manipulation, fast elimination of their immunocomplex and the possibility of being produced in simple expression systems like bacteria and yeast. The increasing demand in antibody based methodologies is driving advances in the production and purification of genetically engineered antibodies and antibody fragments. While advances in expression systems allow the production of high titers of antibodies, there exist some limits imposed by the downstream methodologies which are not efficient enough to ensure their industrialization. The main aim of this review is to highlight the principal characteristics of single-chain variable fragments of antibodies addressing advances and perspectives on scFv purification.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21592
Pellegrini Malpiedi, Luciana; Diaz, César A.; Nerli, Bibiana Beatriz; Pessoa, Adalberto; Single-chain antibody fragments: Purification methodologies; Elsevier; Process Biochemistry; 48; 8; 6-2013; 1242-1251
1359-5113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21592
identifier_str_mv Pellegrini Malpiedi, Luciana; Diaz, César A.; Nerli, Bibiana Beatriz; Pessoa, Adalberto; Single-chain antibody fragments: Purification methodologies; Elsevier; Process Biochemistry; 48; 8; 6-2013; 1242-1251
1359-5113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2013.06.008
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S135951131300278X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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