Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets

Autores
Fariña, Ana Clara; Lavandera, Jimena Veronica; Vera Candioti, Luciana; Suppo, Candela Lujan; Bernal, Claudio Adrian
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: This study aims to investigate if a mixture of functional lipids (FL), containing conjugated linoleic acid (CLA), tocopherols (TP) and phytosterols (PS) prevents some lipid alterations induced by high-fat (HF) diets, without adverse effects. Methods: Male CF1 mice (n=6/group) were fed (4 weeks) with: control (C), HF or HF+FL diets. Results: FL prevented the overweight induced by the HF diet, and reduced the adipose tissues (AT) weight, associated with lower energy efficiency. After the intervention period, the serum triacylglycerol (TAG) levels, in both HF diets underwent a decrease associated with an enhanced LPL activity (mainly in muscle). The beneficial effect of the FL mixture on body weight gain and AT weight, might be attributed to the decreased lipogenesis, denoted by the lower mRNA levels of SREBP1-c and ACC in AT, as well as, by an exacerbated lipid catabolism, reflected by increased mRNA levels of PPARα, ATGL, HSL and UCP2 in adipose tissue. Liver TAG levels were reduced in the HF+FL group due to an elevated lipid oxidation associated with a higher CPT-1 activity and mRNA levels of PPARα and CPT-1a. Moreover, genes linked to fatty acid biosynthesis (SREBP1-c and ACC) showed decreased mRNA levels in both HF diets, this finding being more pronounced in the HF+FL group. Conclusion: The administration of a FL mixture (CLA+TP+PS) prevented some lipid alterations induced by a HF diet, avoiding frequent deleterious effects of CLA in mice through the modulation of gene expression related to the regulation of lipid metabolism.
Fil: Fariña, Ana Clara. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Lavandera, Jimena Veronica. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Vera Candioti, Luciana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Suppo, Candela Lujan. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina
Fil: Bernal, Claudio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina
Materia
CONJUGATED LINOLEIC ACID
TOCOPHEROLS
PHYTOSTEROLS
LIPID METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241633
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/241633
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat dietsFariña, Ana ClaraLavandera, Jimena VeronicaVera Candioti, LucianaSuppo, Candela LujanBernal, Claudio AdrianCONJUGATED LINOLEIC ACIDTOCOPHEROLSPHYTOSTEROLSLIPID METABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: This study aims to investigate if a mixture of functional lipids (FL), containing conjugated linoleic acid (CLA), tocopherols (TP) and phytosterols (PS) prevents some lipid alterations induced by high-fat (HF) diets, without adverse effects. Methods: Male CF1 mice (n=6/group) were fed (4 weeks) with: control (C), HF or HF+FL diets. Results: FL prevented the overweight induced by the HF diet, and reduced the adipose tissues (AT) weight, associated with lower energy efficiency. After the intervention period, the serum triacylglycerol (TAG) levels, in both HF diets underwent a decrease associated with an enhanced LPL activity (mainly in muscle). The beneficial effect of the FL mixture on body weight gain and AT weight, might be attributed to the decreased lipogenesis, denoted by the lower mRNA levels of SREBP1-c and ACC in AT, as well as, by an exacerbated lipid catabolism, reflected by increased mRNA levels of PPARα, ATGL, HSL and UCP2 in adipose tissue. Liver TAG levels were reduced in the HF+FL group due to an elevated lipid oxidation associated with a higher CPT-1 activity and mRNA levels of PPARα and CPT-1a. Moreover, genes linked to fatty acid biosynthesis (SREBP1-c and ACC) showed decreased mRNA levels in both HF diets, this finding being more pronounced in the HF+FL group. Conclusion: The administration of a FL mixture (CLA+TP+PS) prevented some lipid alterations induced by a HF diet, avoiding frequent deleterious effects of CLA in mice through the modulation of gene expression related to the regulation of lipid metabolism.Fil: Fariña, Ana Clara. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Lavandera, Jimena Veronica. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Vera Candioti, Luciana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Suppo, Candela Lujan. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; ArgentinaFil: Bernal, Claudio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; ArgentinaKarger2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241633Fariña, Ana Clara; Lavandera, Jimena Veronica; Vera Candioti, Luciana; Suppo, Candela Lujan; Bernal, Claudio Adrian; Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets; Karger; Lifestyle Genomics; 7-2024; 1-192504-31612504-3188CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://karger.com/doi/10.1159/000539077info:eu-repo/semantics/altIdentifier/doi/10.1159/000539077info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:04:01Zoai:ri.conicet.gov.ar:11336/241633instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:04:01.49CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
title Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
spellingShingle Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
Fariña, Ana Clara
CONJUGATED LINOLEIC ACID
TOCOPHEROLS
PHYTOSTEROLS
LIPID METABOLISM
title_short Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
title_full Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
title_fullStr Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
title_full_unstemmed Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
title_sort Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets
dc.creator.none.fl_str_mv Fariña, Ana Clara
Lavandera, Jimena Veronica
Vera Candioti, Luciana
Suppo, Candela Lujan
Bernal, Claudio Adrian
author Fariña, Ana Clara
author_facet Fariña, Ana Clara
Lavandera, Jimena Veronica
Vera Candioti, Luciana
Suppo, Candela Lujan
Bernal, Claudio Adrian
author_role author
author2 Lavandera, Jimena Veronica
Vera Candioti, Luciana
Suppo, Candela Lujan
Bernal, Claudio Adrian
author2_role author
author
author
author
dc.subject.none.fl_str_mv CONJUGATED LINOLEIC ACID
TOCOPHEROLS
PHYTOSTEROLS
LIPID METABOLISM
topic CONJUGATED LINOLEIC ACID
TOCOPHEROLS
PHYTOSTEROLS
LIPID METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: This study aims to investigate if a mixture of functional lipids (FL), containing conjugated linoleic acid (CLA), tocopherols (TP) and phytosterols (PS) prevents some lipid alterations induced by high-fat (HF) diets, without adverse effects. Methods: Male CF1 mice (n=6/group) were fed (4 weeks) with: control (C), HF or HF+FL diets. Results: FL prevented the overweight induced by the HF diet, and reduced the adipose tissues (AT) weight, associated with lower energy efficiency. After the intervention period, the serum triacylglycerol (TAG) levels, in both HF diets underwent a decrease associated with an enhanced LPL activity (mainly in muscle). The beneficial effect of the FL mixture on body weight gain and AT weight, might be attributed to the decreased lipogenesis, denoted by the lower mRNA levels of SREBP1-c and ACC in AT, as well as, by an exacerbated lipid catabolism, reflected by increased mRNA levels of PPARα, ATGL, HSL and UCP2 in adipose tissue. Liver TAG levels were reduced in the HF+FL group due to an elevated lipid oxidation associated with a higher CPT-1 activity and mRNA levels of PPARα and CPT-1a. Moreover, genes linked to fatty acid biosynthesis (SREBP1-c and ACC) showed decreased mRNA levels in both HF diets, this finding being more pronounced in the HF+FL group. Conclusion: The administration of a FL mixture (CLA+TP+PS) prevented some lipid alterations induced by a HF diet, avoiding frequent deleterious effects of CLA in mice through the modulation of gene expression related to the regulation of lipid metabolism.
Fil: Fariña, Ana Clara. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Lavandera, Jimena Veronica. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Vera Candioti, Luciana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Suppo, Candela Lujan. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina
Fil: Bernal, Claudio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas; Argentina
description Introduction: This study aims to investigate if a mixture of functional lipids (FL), containing conjugated linoleic acid (CLA), tocopherols (TP) and phytosterols (PS) prevents some lipid alterations induced by high-fat (HF) diets, without adverse effects. Methods: Male CF1 mice (n=6/group) were fed (4 weeks) with: control (C), HF or HF+FL diets. Results: FL prevented the overweight induced by the HF diet, and reduced the adipose tissues (AT) weight, associated with lower energy efficiency. After the intervention period, the serum triacylglycerol (TAG) levels, in both HF diets underwent a decrease associated with an enhanced LPL activity (mainly in muscle). The beneficial effect of the FL mixture on body weight gain and AT weight, might be attributed to the decreased lipogenesis, denoted by the lower mRNA levels of SREBP1-c and ACC in AT, as well as, by an exacerbated lipid catabolism, reflected by increased mRNA levels of PPARα, ATGL, HSL and UCP2 in adipose tissue. Liver TAG levels were reduced in the HF+FL group due to an elevated lipid oxidation associated with a higher CPT-1 activity and mRNA levels of PPARα and CPT-1a. Moreover, genes linked to fatty acid biosynthesis (SREBP1-c and ACC) showed decreased mRNA levels in both HF diets, this finding being more pronounced in the HF+FL group. Conclusion: The administration of a FL mixture (CLA+TP+PS) prevented some lipid alterations induced by a HF diet, avoiding frequent deleterious effects of CLA in mice through the modulation of gene expression related to the regulation of lipid metabolism.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241633
Fariña, Ana Clara; Lavandera, Jimena Veronica; Vera Candioti, Luciana; Suppo, Candela Lujan; Bernal, Claudio Adrian; Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets; Karger; Lifestyle Genomics; 7-2024; 1-19
2504-3161
2504-3188
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241633
identifier_str_mv Fariña, Ana Clara; Lavandera, Jimena Veronica; Vera Candioti, Luciana; Suppo, Candela Lujan; Bernal, Claudio Adrian; Nutriomic effects of precision lipids on murine hepatic triacylglycerol alterations induced by high-fat diets; Karger; Lifestyle Genomics; 7-2024; 1-19
2504-3161
2504-3188
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://karger.com/doi/10.1159/000539077
info:eu-repo/semantics/altIdentifier/doi/10.1159/000539077
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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