Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition

Autores
da Silva Sabo, Sabrina; Mendes, Maria Anita; da Silva Araújo, Elias; Bicudo de Almeida Muradian, Ligia; Makiyama, Edson Naoto; Leblanc, Jean Guy Joseph; Borelli, Primavera; Fock, Ricardo Ambrósio; Knöbl, Terezinha; Pinheiro de Souza Oliveira, Ricardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The demand for animal protein for human consumption has been risen exponentially. Modern animal production practices are associated with the regular use of antibiotics, potentially increasing the emerging multi-resistant bacteria, which may have a negative impact on public health. In poultry production, substances capable of maximizing the animals’ performance and displaying an antimicrobial activity against pathogens are very well desirable features. Probiotic can be an efcient solution for such a task. In the present work, lactic acid bacteria (LAB) were isolated from chicken cecum and screened for their antagonistic efect towards many pathogens. Their capacity of producing the B-complex vitamins folate and ribofavin were also evaluated. From 314 isolates, three (C43, C175 and C195) produced Bacteriocin-Like Inhibitory Substances (BLIS) against Staphylococcus aureus (inhibition zones of 18.9, 21.5, 19.5mm, respectively) and also inhibited the growth of Salmonella Heidelberg. The isolate C43 was identifed as Enterococcus faecium, while C173 and C195 were both identifed as Lactococcus lactis subsp. lactis. Moreover, the isolates L. lactis subsp. lactis strains C173 and C195 demonstrated high potential to be used as probiotic in poultry feed, in addition to their advantage of producing folate (58.0 and 595.5ng/ mL, respectively) and ribofavin (223.3 and 175.0ng/mL, respectively).
Fil: da Silva Sabo, Sabrina. Universidade de Sao Paulo; Brasil
Fil: Mendes, Maria Anita. Universidade de Sao Paulo; Brasil
Fil: da Silva Araújo, Elias. Universidade de Sao Paulo; Brasil
Fil: Bicudo de Almeida Muradian, Ligia. Universidade de Sao Paulo; Brasil
Fil: Makiyama, Edson Naoto. Universidade de Sao Paulo; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Borelli, Primavera. Universidade de Sao Paulo; Brasil
Fil: Fock, Ricardo Ambrósio. Universidade de Sao Paulo; Brasil
Fil: Knöbl, Terezinha. Universidade de Sao Paulo; Brasil
Fil: Pinheiro de Souza Oliveira, Ricardo. Universidade de Sao Paulo; Brasil
Materia
LACTIC ACID BACTERIA
PROBIOTICS
POULTRY
BACTERIOCINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112386
Acceder
id CONICETDig_13b9dddfbfb9cae0f76d397d81704212
oai_identifier_str oai:ri.conicet.gov.ar:11336/112386
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutritionda Silva Sabo, SabrinaMendes, Maria Anitada Silva Araújo, EliasBicudo de Almeida Muradian, LigiaMakiyama, Edson NaotoLeblanc, Jean Guy JosephBorelli, PrimaveraFock, Ricardo AmbrósioKnöbl, TerezinhaPinheiro de Souza Oliveira, RicardoLACTIC ACID BACTERIAPROBIOTICSPOULTRYBACTERIOCINShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The demand for animal protein for human consumption has been risen exponentially. Modern animal production practices are associated with the regular use of antibiotics, potentially increasing the emerging multi-resistant bacteria, which may have a negative impact on public health. In poultry production, substances capable of maximizing the animals’ performance and displaying an antimicrobial activity against pathogens are very well desirable features. Probiotic can be an efcient solution for such a task. In the present work, lactic acid bacteria (LAB) were isolated from chicken cecum and screened for their antagonistic efect towards many pathogens. Their capacity of producing the B-complex vitamins folate and ribofavin were also evaluated. From 314 isolates, three (C43, C175 and C195) produced Bacteriocin-Like Inhibitory Substances (BLIS) against Staphylococcus aureus (inhibition zones of 18.9, 21.5, 19.5mm, respectively) and also inhibited the growth of Salmonella Heidelberg. The isolate C43 was identifed as Enterococcus faecium, while C173 and C195 were both identifed as Lactococcus lactis subsp. lactis. Moreover, the isolates L. lactis subsp. lactis strains C173 and C195 demonstrated high potential to be used as probiotic in poultry feed, in addition to their advantage of producing folate (58.0 and 595.5ng/ mL, respectively) and ribofavin (223.3 and 175.0ng/mL, respectively).Fil: da Silva Sabo, Sabrina. Universidade de Sao Paulo; BrasilFil: Mendes, Maria Anita. Universidade de Sao Paulo; BrasilFil: da Silva Araújo, Elias. Universidade de Sao Paulo; BrasilFil: Bicudo de Almeida Muradian, Ligia. Universidade de Sao Paulo; BrasilFil: Makiyama, Edson Naoto. Universidade de Sao Paulo; BrasilFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Borelli, Primavera. Universidade de Sao Paulo; BrasilFil: Fock, Ricardo Ambrósio. Universidade de Sao Paulo; BrasilFil: Knöbl, Terezinha. Universidade de Sao Paulo; BrasilFil: Pinheiro de Souza Oliveira, Ricardo. Universidade de Sao Paulo; BrasilNature Publishing Group2020-04-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112386da Silva Sabo, Sabrina; Mendes, Maria Anita; da Silva Araújo, Elias; Bicudo de Almeida Muradian, Ligia; Makiyama, Edson Naoto; et al.; Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition; Nature Publishing Group; Scientific Reports; 10; 1; 29-4-2020; 1-14;7235-72352045-23222045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-64038-9info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-64038-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:42Zoai:ri.conicet.gov.ar:11336/112386instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:42.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
title Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
spellingShingle Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
da Silva Sabo, Sabrina
LACTIC ACID BACTERIA
PROBIOTICS
POULTRY
BACTERIOCINS
title_short Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
title_full Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
title_fullStr Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
title_full_unstemmed Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
title_sort Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition
dc.creator.none.fl_str_mv da Silva Sabo, Sabrina
Mendes, Maria Anita
da Silva Araújo, Elias
Bicudo de Almeida Muradian, Ligia
Makiyama, Edson Naoto
Leblanc, Jean Guy Joseph
Borelli, Primavera
Fock, Ricardo Ambrósio
Knöbl, Terezinha
Pinheiro de Souza Oliveira, Ricardo
author da Silva Sabo, Sabrina
author_facet da Silva Sabo, Sabrina
Mendes, Maria Anita
da Silva Araújo, Elias
Bicudo de Almeida Muradian, Ligia
Makiyama, Edson Naoto
Leblanc, Jean Guy Joseph
Borelli, Primavera
Fock, Ricardo Ambrósio
Knöbl, Terezinha
Pinheiro de Souza Oliveira, Ricardo
author_role author
author2 Mendes, Maria Anita
da Silva Araújo, Elias
Bicudo de Almeida Muradian, Ligia
Makiyama, Edson Naoto
Leblanc, Jean Guy Joseph
Borelli, Primavera
Fock, Ricardo Ambrósio
Knöbl, Terezinha
Pinheiro de Souza Oliveira, Ricardo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LACTIC ACID BACTERIA
PROBIOTICS
POULTRY
BACTERIOCINS
topic LACTIC ACID BACTERIA
PROBIOTICS
POULTRY
BACTERIOCINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The demand for animal protein for human consumption has been risen exponentially. Modern animal production practices are associated with the regular use of antibiotics, potentially increasing the emerging multi-resistant bacteria, which may have a negative impact on public health. In poultry production, substances capable of maximizing the animals’ performance and displaying an antimicrobial activity against pathogens are very well desirable features. Probiotic can be an efcient solution for such a task. In the present work, lactic acid bacteria (LAB) were isolated from chicken cecum and screened for their antagonistic efect towards many pathogens. Their capacity of producing the B-complex vitamins folate and ribofavin were also evaluated. From 314 isolates, three (C43, C175 and C195) produced Bacteriocin-Like Inhibitory Substances (BLIS) against Staphylococcus aureus (inhibition zones of 18.9, 21.5, 19.5mm, respectively) and also inhibited the growth of Salmonella Heidelberg. The isolate C43 was identifed as Enterococcus faecium, while C173 and C195 were both identifed as Lactococcus lactis subsp. lactis. Moreover, the isolates L. lactis subsp. lactis strains C173 and C195 demonstrated high potential to be used as probiotic in poultry feed, in addition to their advantage of producing folate (58.0 and 595.5ng/ mL, respectively) and ribofavin (223.3 and 175.0ng/mL, respectively).
Fil: da Silva Sabo, Sabrina. Universidade de Sao Paulo; Brasil
Fil: Mendes, Maria Anita. Universidade de Sao Paulo; Brasil
Fil: da Silva Araújo, Elias. Universidade de Sao Paulo; Brasil
Fil: Bicudo de Almeida Muradian, Ligia. Universidade de Sao Paulo; Brasil
Fil: Makiyama, Edson Naoto. Universidade de Sao Paulo; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Borelli, Primavera. Universidade de Sao Paulo; Brasil
Fil: Fock, Ricardo Ambrósio. Universidade de Sao Paulo; Brasil
Fil: Knöbl, Terezinha. Universidade de Sao Paulo; Brasil
Fil: Pinheiro de Souza Oliveira, Ricardo. Universidade de Sao Paulo; Brasil
description The demand for animal protein for human consumption has been risen exponentially. Modern animal production practices are associated with the regular use of antibiotics, potentially increasing the emerging multi-resistant bacteria, which may have a negative impact on public health. In poultry production, substances capable of maximizing the animals’ performance and displaying an antimicrobial activity against pathogens are very well desirable features. Probiotic can be an efcient solution for such a task. In the present work, lactic acid bacteria (LAB) were isolated from chicken cecum and screened for their antagonistic efect towards many pathogens. Their capacity of producing the B-complex vitamins folate and ribofavin were also evaluated. From 314 isolates, three (C43, C175 and C195) produced Bacteriocin-Like Inhibitory Substances (BLIS) against Staphylococcus aureus (inhibition zones of 18.9, 21.5, 19.5mm, respectively) and also inhibited the growth of Salmonella Heidelberg. The isolate C43 was identifed as Enterococcus faecium, while C173 and C195 were both identifed as Lactococcus lactis subsp. lactis. Moreover, the isolates L. lactis subsp. lactis strains C173 and C195 demonstrated high potential to be used as probiotic in poultry feed, in addition to their advantage of producing folate (58.0 and 595.5ng/ mL, respectively) and ribofavin (223.3 and 175.0ng/mL, respectively).
publishDate 2020
dc.date.none.fl_str_mv 2020-04-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112386
da Silva Sabo, Sabrina; Mendes, Maria Anita; da Silva Araújo, Elias; Bicudo de Almeida Muradian, Ligia; Makiyama, Edson Naoto; et al.; Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition; Nature Publishing Group; Scientific Reports; 10; 1; 29-4-2020; 1-14;7235-7235
2045-2322
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112386
identifier_str_mv da Silva Sabo, Sabrina; Mendes, Maria Anita; da Silva Araújo, Elias; Bicudo de Almeida Muradian, Ligia; Makiyama, Edson Naoto; et al.; Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition; Nature Publishing Group; Scientific Reports; 10; 1; 29-4-2020; 1-14;7235-7235
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-64038-9
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-64038-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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