Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
- Autores
- Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples.
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Danert, Fátima Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina - Materia
-
Propolis
Flavonoid Phenolic Precipitation
Total Phenolic Compounds Content
Determination of Phenolic Non Flavonoides - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/6872
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Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extractsIsla, Maria InesSalas, Ana LiliaDanert, Fátima CarolinaZampini, Iris CatianaOrdóñez, Roxana MabelPropolisFlavonoid Phenolic PrecipitationTotal Phenolic Compounds ContentDetermination of Phenolic Non Flavonoideshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples.Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; ArgentinaFil: Danert, Fátima Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaTaylor & Francis2014-02-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6872Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel; Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts; Taylor & Francis; Pharmaceutical Biology; 52; 7; 7-2-2014; 835-8401388-0209enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.3109/13880209.2013.871638info:eu-repo/semantics/altIdentifier/pmid/24920228info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/13880209.2013.871638?journalCode=iphb20#.V6CoT2VXui4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:13Zoai:ri.conicet.gov.ar:11336/6872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:13.797CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts |
title |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts |
spellingShingle |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts Isla, Maria Ines Propolis Flavonoid Phenolic Precipitation Total Phenolic Compounds Content Determination of Phenolic Non Flavonoides |
title_short |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts |
title_full |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts |
title_fullStr |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts |
title_full_unstemmed |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts |
title_sort |
Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts |
dc.creator.none.fl_str_mv |
Isla, Maria Ines Salas, Ana Lilia Danert, Fátima Carolina Zampini, Iris Catiana Ordóñez, Roxana Mabel |
author |
Isla, Maria Ines |
author_facet |
Isla, Maria Ines Salas, Ana Lilia Danert, Fátima Carolina Zampini, Iris Catiana Ordóñez, Roxana Mabel |
author_role |
author |
author2 |
Salas, Ana Lilia Danert, Fátima Carolina Zampini, Iris Catiana Ordóñez, Roxana Mabel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Propolis Flavonoid Phenolic Precipitation Total Phenolic Compounds Content Determination of Phenolic Non Flavonoides |
topic |
Propolis Flavonoid Phenolic Precipitation Total Phenolic Compounds Content Determination of Phenolic Non Flavonoides |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples. Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina Fil: Danert, Fátima Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina |
description |
Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/6872 Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel; Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts; Taylor & Francis; Pharmaceutical Biology; 52; 7; 7-2-2014; 835-840 1388-0209 |
url |
http://hdl.handle.net/11336/6872 |
identifier_str_mv |
Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel; Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts; Taylor & Francis; Pharmaceutical Biology; 52; 7; 7-2-2014; 835-840 1388-0209 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ info:eu-repo/semantics/altIdentifier/doi/10.3109/13880209.2013.871638 info:eu-repo/semantics/altIdentifier/pmid/24920228 info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/13880209.2013.871638?journalCode=iphb20#.V6CoT2VXui4 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |