Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts

Autores
Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples.
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Danert, Fátima Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Materia
Propolis
Flavonoid Phenolic Precipitation
Total Phenolic Compounds Content
Determination of Phenolic Non Flavonoides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6872
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extractsIsla, Maria InesSalas, Ana LiliaDanert, Fátima CarolinaZampini, Iris CatianaOrdóñez, Roxana MabelPropolisFlavonoid Phenolic PrecipitationTotal Phenolic Compounds ContentDetermination of Phenolic Non Flavonoideshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples.Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; ArgentinaFil: Danert, Fátima Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaTaylor & Francis2014-02-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6872Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel; Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts; Taylor & Francis; Pharmaceutical Biology; 52; 7; 7-2-2014; 835-8401388-0209enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.3109/13880209.2013.871638info:eu-repo/semantics/altIdentifier/pmid/24920228info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/13880209.2013.871638?journalCode=iphb20#.V6CoT2VXui4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:37:30Zoai:ri.conicet.gov.ar:11336/6872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:37:30.543CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
title Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
spellingShingle Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
Isla, Maria Ines
Propolis
Flavonoid Phenolic Precipitation
Total Phenolic Compounds Content
Determination of Phenolic Non Flavonoides
title_short Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
title_full Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
title_fullStr Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
title_full_unstemmed Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
title_sort Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts
dc.creator.none.fl_str_mv Isla, Maria Ines
Salas, Ana Lilia
Danert, Fátima Carolina
Zampini, Iris Catiana
Ordóñez, Roxana Mabel
author Isla, Maria Ines
author_facet Isla, Maria Ines
Salas, Ana Lilia
Danert, Fátima Carolina
Zampini, Iris Catiana
Ordóñez, Roxana Mabel
author_role author
author2 Salas, Ana Lilia
Danert, Fátima Carolina
Zampini, Iris Catiana
Ordóñez, Roxana Mabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Propolis
Flavonoid Phenolic Precipitation
Total Phenolic Compounds Content
Determination of Phenolic Non Flavonoides
topic Propolis
Flavonoid Phenolic Precipitation
Total Phenolic Compounds Content
Determination of Phenolic Non Flavonoides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples.
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Danert, Fátima Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
description Context: Traditionally, the content of total phenolics (flavonoid phenolics (FP) and nonflavonoid phenolics (NFP)) and flavonoids (flavone/flavonol and flavonone/dihydroflavonol)in propolis has been determined by different methodologies. Until now, the percentage of total phenolic (TP) compounds that corresponds to FP and NFP, expressed in the same units by a spectrophotometric method, has not been determined. Objective: The current study proposes a quick and simple methodology that separates FP and NFP in propolis samples and determines TP, FP, and NFP by the same method. Materials and methods: Propolis samples from five Argentine provinces (Tucuma´n, Santiago del Estero, Salta, Misiones, and Jujuy) were used. Extraction of TP from the propolis samples was carried out by maceration with 80% ethanol and quantified by Folin?Ciocalteu reagent (FC-R). Then, FP was precipitated with formaldehyde in acid medium. After centrifugation, NFP were determined in the supernatant using FC-R. FP content was calculated as the difference between the content of TP and NFP. The method was also validated using commercial flavonoids and chalcones. Results: FP recovery in all experiments was between 85.95% and 98.29%. Propolis from Tucuman had significantly higher amounts of total phenols than propolis from other provinces. SE5 showed higher content of FP (81.52%) followed by SA1 (74.75%). The propolis from TUC4, SA4, SE3, and MI showed the lowest FP content and highest content of NFP. Conclusions: This method provides a simple, reliable, and specific spectrophotometric assay to estimate the content of NFP, FP, and TP in propolis samples.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6872
Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel; Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts; Taylor & Francis; Pharmaceutical Biology; 52; 7; 7-2-2014; 835-840
1388-0209
url http://hdl.handle.net/11336/6872
identifier_str_mv Isla, Maria Ines; Salas, Ana Lilia; Danert, Fátima Carolina; Zampini, Iris Catiana; Ordóñez, Roxana Mabel; Analytical methodology optimization to estimate the content of non-flavonoid phenolic compounds in Argentine propolis extracts; Taylor & Francis; Pharmaceutical Biology; 52; 7; 7-2-2014; 835-840
1388-0209
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.3109/13880209.2013.871638
info:eu-repo/semantics/altIdentifier/pmid/24920228
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/13880209.2013.871638?journalCode=iphb20#.V6CoT2VXui4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
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application/pdf
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dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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